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Plants 2018, 7(4), 93; https://doi.org/10.3390/plants7040093

Revised and Improved Procedure for Immunolocalization of Male Meiotic Chromosomal Proteins and Spindle in Plants without the Use of Enzymes

1
Department of Radiation Oncology, James Cancer Hospital and Comprehensive Cancer Center, The Ohio State University Wexner School of Medicine, Columbus, OH 43210, USA
2
State Key Laboratory of Crop Stress Biology for Arid areas, College of Horticulture, Northwest A&F University, Yangling 712100, China
3
Hughes Laboratories, Department of Chemistry and Biochemistry, Miami University, Oxford, OH 45056, USA
*
Author to whom correspondence should be addressed.
Received: 1 October 2018 / Revised: 23 October 2018 / Accepted: 24 October 2018 / Published: 29 October 2018
(This article belongs to the Special Issue Plant Reproduction)
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Abstract

Immunolocalization studies to visualize the distribution of proteins on meiotic chromosomes have become an integral part of studies on meiosis in the model organism Arabidopsis thaliana. These techniques have been used to visualize a wide range of meiotic proteins involved in different aspects of meiosis, including sister chromatid cohesion, recombination, synapsis, and chromosome segregation. However, the analysis of meiotic spindle structure by immunofluorescence is of outstanding importance in plant reproductive biology and is very challenging. In the following report, we describe the complete and easy protocol for the localization of proteins to the male meiotic spindle and male meiotic chromosomes. The protocol is fast, improved, and robust without the use of any harsh enzymes. View Full-Text
Keywords: Arabidopsis; spindle; synapsis; cohesin; meiosis; immunofluorescence Arabidopsis; spindle; synapsis; cohesin; meiosis; immunofluorescence
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De, K.; Yuan, L.; Makaroff, C.A. Revised and Improved Procedure for Immunolocalization of Male Meiotic Chromosomal Proteins and Spindle in Plants without the Use of Enzymes. Plants 2018, 7, 93.

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