A Cyclic Nucleotide-Gated Channel, HvCNGC2-3, Is Activated by the Co-Presence of Na+ and K+ and Permeable to Na+ and K+ Non-Selectively

Mori, Izumi C.; Nobukiyo, Yuichi; Nakahara, Yoshiki; Shibasaka, Mineo; Furuichi, Takuya; Katsuhara, Maki

doi:10.3390/plants7030061

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A Cyclic Nucleotide-Gated Channel, HvCNGC2-3, Is Activated by the Co-Presence of Na⁺ and K⁺ and Permeable to Na⁺ and K⁺ Non-Selectively

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Institute of Plant Science and Resources, Okayama University, 2-20-1 Chuo, Kurashiki 710-0046, Japan

Department of Human Life Science, Nagoya University of Economics, 61-1 Uchikubo, Inuyama 484-8504, Japan

Author to whom correspondence should be addressed.

Plants 2018, 7(3), 61; https://doi.org/10.3390/plants7030061

Received: 11 June 2018 / Revised: 9 July 2018 / Accepted: 24 July 2018 / Published: 26 July 2018

(This article belongs to the Special Issue Salt and Water Stress Tolerance in Plants)

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Abstract

Cyclic nucleotide-gated channels (CNGCs) have been postulated to contribute significantly in plant development and stress resistance. However, their electrophysiological properties remain poorly understood. Here, we characterized barley CNGC2-3 (HvCNGC2-3) by the two-electrode voltage-clamp technique in the Xenopus laevis oocyte heterologous expression system. Current was not observed in X. laevis oocytes injected with HvCNGC2-3 complementary RNA (cRNA) in a bathing solution containing either Na⁺ or K⁺ solely, even in the presence of 8-bromoadenosine 3′,5′-cyclic monophosphate (8Br-cAMP) or 8-bromoguanosine 3′,5′-cyclic monophosphate (8Br-cGMP). A weakly voltage-dependent slow hyperpolarization-activated ion current was observed in the co-presence of Na⁺ and K⁺ in the bathing solution and in the presence of 10 µM 8Br-cAMP, but not 8Br-cGMP. Permeability ratios of HvCNGC2-3 to K⁺, Na⁺ and Cl⁻ were determined as 1:0.63:0.03 according to reversal-potential analyses. Amino-acid replacement of the unique ion-selective motif of HvCNGC2-3, AQGL, with the canonical motif, GQGL, resulted in the abolition of the current. This study reports a unique two-ion-dependent activation characteristic of the barley CNGC, HvCNGC2-3. View Full-Text

Keywords: cyclic nucleotide-gated channel; barley; sodium; potassium; roots

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MDPI and ACS Style

Mori, I.C.; Nobukiyo, Y.; Nakahara, Y.; Shibasaka, M.; Furuichi, T.; Katsuhara, M. A Cyclic Nucleotide-Gated Channel, HvCNGC2-3, Is Activated by the Co-Presence of Na⁺ and K⁺ and Permeable to Na⁺ and K⁺ Non-Selectively. Plants 2018, 7, 61. https://doi.org/10.3390/plants7030061

AMA Style

Mori IC, Nobukiyo Y, Nakahara Y, Shibasaka M, Furuichi T, Katsuhara M. A Cyclic Nucleotide-Gated Channel, HvCNGC2-3, Is Activated by the Co-Presence of Na⁺ and K⁺ and Permeable to Na⁺ and K⁺ Non-Selectively. Plants. 2018; 7(3):61. https://doi.org/10.3390/plants7030061

Chicago/Turabian Style

Mori, Izumi C., Yuichi Nobukiyo, Yoshiki Nakahara, Mineo Shibasaka, Takuya Furuichi, and Maki Katsuhara. 2018. "A Cyclic Nucleotide-Gated Channel, HvCNGC2-3, Is Activated by the Co-Presence of Na⁺ and K⁺ and Permeable to Na⁺ and K⁺ Non-Selectively" Plants 7, no. 3: 61. https://doi.org/10.3390/plants7030061

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A Cyclic Nucleotide-Gated Channel, HvCNGC2-3, Is Activated by the Co-Presence of Na⁺ and K⁺ and Permeable to Na⁺ and K⁺ Non-Selectively

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