Identification of the Abundant Hydroxyproline-Rich Glycoproteins in the Root Walls of Wild-Type Arabidopsis, an ext3 Mutant Line, and Its Phenotypic Revertant
1
Department of Chemistry and Biochemistry, Ohio University, Athens, OH 45701, USA
2
Department of Biochemistry and Molecular Biology, University of Massachusetts, Amherst, MA 01003, USA
3
Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, OK 74078, USA
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Academic Editor: Philip J. Harris
Plants 2015, 4(1), 85-111; https://doi.org/10.3390/plants4010085
Received: 9 November 2014 / Accepted: 15 January 2015 / Published: 21 January 2015
(This article belongs to the Special Issue Plant Cell Walls: Chemical and Metabolic Analysis)
Abstract
Extensins are members of the cell wall hydroxyproline-rich glycoprotein (HRGP) superfamily that form covalently cross-linked networks in primary cell walls. A knockout mutation in EXT3 (AT1G21310), the gene coding EXTENSIN 3 (EXT3) in Arabidopsis Landsberg erecta resulted in a lethal phenotype, although about 20% of the knockout plants have an apparently normal phenotype (ANP). In this study the root cell wall HRGP components of wild-type, ANP and the ext3 mutant seedlings were characterized by peptide fractionation of trypsin digested anhydrous hydrogen fluoride deglycosylated wall residues and by sequencing using LC-MS/MS. Several HRGPs, including EXT3, were identified in the wild-type root walls but not in walls of the ANP and lethal mutant. Indeed the ANP walls and walls of mutants displaying the lethal phenotype possessed HRGPs, but the profiles suggest that changes in the amount and perhaps type may account for the corresponding phenotypes. View Full-TextKeywords:
Arabidopsis root; cell wall; hydroxyproline-rich glycoprotein; Extensin 3; LRX; PRP4; proteomics
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MDPI and ACS Style
Chen, Y.; Ye, D.; Held, M.A.; Cannon, M.C.; Ray, T.; Saha, P.; Frye, A.N.; Mort, A.J.; Kieliszewski, M.J. Identification of the Abundant Hydroxyproline-Rich Glycoproteins in the Root Walls of Wild-Type Arabidopsis, an ext3 Mutant Line, and Its Phenotypic Revertant. Plants 2015, 4, 85-111.