Breeding and Molecular Characterization of Insect-Resistant Transgenic Cotton

Zhang, Xiaochun; Yang, Jiangtao; Chen, Yuxiao; Wang, Mengyu; Zhang, Xuanming; Shen, Mingni; Zhang, Shuo; Wang, Zhixing; Wang, Xujing

doi:10.3390/plants15101551

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Open AccessArticle

Breeding and Molecular Characterization of Insect-Resistant Transgenic Cotton

by

Xiaochun Zhang

,

Jiangtao Yang

,

Yuxiao Chen

,

Mengyu Wang

,

Xuanming Zhang

,

Mingni Shen

,

Shuo Zhang

,

Zhixing Wang

^* and

Xujing Wang

^*

Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China

^*

Authors to whom correspondence should be addressed.

Plants 2026, 15(10), 1551; https://doi.org/10.3390/plants15101551

Submission received: 12 April 2026 / Revised: 4 May 2026 / Accepted: 15 May 2026 / Published: 19 May 2026

(This article belongs to the Section Plant Molecular Biology)

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Abstract

Cotton is one of the world’s important cash crops and occupies a significant position in agricultural production and the national economy. However, insect pests severely affect the growth, yield and quality of cotton. To ensure high and stable cotton yields, the cultivation of insect-resistant transgenic cotton via transgenic technology can not only effectively reduce the impact of chemical pesticides on crops but also exert excellent control effects against pests such as cotton bollworms. In this study, the plant expression vector pC2300-VEC harboring the target genes epsps, cry1Ac and vip3A was introduced into the genome of the recipient cotton cultivar CCRI 24 via Agrobacterium-mediated transformation. The obtained transgenic cotton plants were subjected to the identification of target genes and target traits, and the insect-resistant transgenic cotton line BrsC35 was ultimately obtained. PacBio sequencing combined with conventional molecular characterization methods was used to analyze its insertion site, copy number and other characteristics, providing a new germplasm for insect-resistant transgenic cotton.

Keywords: transgenic cotton; insect-resistant; molecular characteristics

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MDPI and ACS Style

Zhang, X.; Yang, J.; Chen, Y.; Wang, M.; Zhang, X.; Shen, M.; Zhang, S.; Wang, Z.; Wang, X. Breeding and Molecular Characterization of Insect-Resistant Transgenic Cotton. Plants 2026, 15, 1551. https://doi.org/10.3390/plants15101551

AMA Style

Zhang X, Yang J, Chen Y, Wang M, Zhang X, Shen M, Zhang S, Wang Z, Wang X. Breeding and Molecular Characterization of Insect-Resistant Transgenic Cotton. Plants. 2026; 15(10):1551. https://doi.org/10.3390/plants15101551

Chicago/Turabian Style

Zhang, Xiaochun, Jiangtao Yang, Yuxiao Chen, Mengyu Wang, Xuanming Zhang, Mingni Shen, Shuo Zhang, Zhixing Wang, and Xujing Wang. 2026. "Breeding and Molecular Characterization of Insect-Resistant Transgenic Cotton" Plants 15, no. 10: 1551. https://doi.org/10.3390/plants15101551

APA Style

Zhang, X., Yang, J., Chen, Y., Wang, M., Zhang, X., Shen, M., Zhang, S., Wang, Z., & Wang, X. (2026). Breeding and Molecular Characterization of Insect-Resistant Transgenic Cotton. Plants, 15(10), 1551. https://doi.org/10.3390/plants15101551

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Breeding and Molecular Characterization of Insect-Resistant Transgenic Cotton

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