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Volume 14
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10.3390/plants14233612
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This is an early access version, the complete PDF, HTML, and XML versions will be available soon.
Article

Transcription Factor GmMYB29 Activates GmPP2C-37like Expression to Mediate Soybean Defense Against Heterodera glycines Race 3

by
Shuo Qu
1,†,
Shihao Hu
1,†,
Gengchen Song
1,
Miaoli Zhang
1,
Yingpeng Han
1,
Weili Teng
1,
Yongguang Li
1,
Hui Wang
2,*,
Haiyan Li
1,* and
Xue Zhao
1,*
1
Key Laboratory of Soybean Biology in Chinese Ministry of Education (Key Laboratory of Soybean Biology and Breeding/Genetics of Chinese Agriculture Ministry), Northeast Agricultural University, Harbin 150030, China
2
National Center for Soybean Improvement, State Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, Nanjing Agricultural University, Nanjing 210095, China
*
Authors to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Plants 2025, 14(23), 3612; https://doi.org/10.3390/plants14233612
Submission received: 23 October 2025 / Revised: 8 November 2025 / Accepted: 25 November 2025 / Published: 26 November 2025
(This article belongs to the Special Issue Crop Germplasm Resources, Genomics, and Molecular Breeding)
Versions Notes

Abstract

Soybean cyst nematode (SCN, Heterodera glycines) is one of the major pathogens of soybean worldwide. We utilized the CHIP-Seq (chromatin immunoprecipitation sequencing) and RNA-Seq (RNA sequencing) data from the transgenic GmMYB29 strain (Glycine Max roots). We then performed enrichment analysis using KEGG and GO to identify potential candidate genes within the promoter-binding region. A targeted regulatory relationship between the GmMYB29 and GmPP2C-37like genes was further identified using the dual-luciferase Assay (Luciferase, LUC) and yeast one-hybrid Assay (Y1H). Hairy roots with target gene overexpression and gene-edited hairy roots were generated, and their resistance to soybean cyst nematode (SCN) was evaluated. Meanwhile, the presence of reciprocal genes with GmPP2C-37like was determined by the yeast two-hybrid library screening method. The targeting relationship between GmMYB29 and GmPP2C-37like genes was further validated through the Y1H assay and LUC assay. Based on phenotypic assessments of SCN, transgenic soybean roots overexpressing GmPP2C-37like exhibited significantly enhanced resistance to SCN 3 compared to wild-type. Further analysis revealed that GmPP2C-37like collaborates with other regulatory factors to modulate soybean resistance against SCN. Yeast two-hybrid library (Y2H) screening identified 18 interacting proteins. These findings not only illuminate the functional role of GmPP2C-37like but also provide a foundation for dissecting its molecular network. Moreover, the results offer promising candidate genes for enhancing SCN resistance and optimizing soybean resilience through targeted genetic strategies.
Keywords: GmMYB29; GmPP2C-37like; gene regulation; SCN resistance; soybean cyst nematode GmMYB29; GmPP2C-37like; gene regulation; SCN resistance; soybean cyst nematode

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MDPI and ACS Style

Qu, S.; Hu, S.; Song, G.; Zhang, M.; Han, Y.; Teng, W.; Li, Y.; Wang, H.; Li, H.; Zhao, X. Transcription Factor GmMYB29 Activates GmPP2C-37like Expression to Mediate Soybean Defense Against Heterodera glycines Race 3. Plants 2025, 14, 3612. https://doi.org/10.3390/plants14233612

AMA Style

Qu S, Hu S, Song G, Zhang M, Han Y, Teng W, Li Y, Wang H, Li H, Zhao X. Transcription Factor GmMYB29 Activates GmPP2C-37like Expression to Mediate Soybean Defense Against Heterodera glycines Race 3. Plants. 2025; 14(23):3612. https://doi.org/10.3390/plants14233612

Chicago/Turabian Style

Qu, Shuo, Shihao Hu, Gengchen Song, Miaoli Zhang, Yingpeng Han, Weili Teng, Yongguang Li, Hui Wang, Haiyan Li, and Xue Zhao. 2025. "Transcription Factor GmMYB29 Activates GmPP2C-37like Expression to Mediate Soybean Defense Against Heterodera glycines Race 3" Plants 14, no. 23: 3612. https://doi.org/10.3390/plants14233612

APA Style

Qu, S., Hu, S., Song, G., Zhang, M., Han, Y., Teng, W., Li, Y., Wang, H., Li, H., & Zhao, X. (2025). Transcription Factor GmMYB29 Activates GmPP2C-37like Expression to Mediate Soybean Defense Against Heterodera glycines Race 3. Plants, 14(23), 3612. https://doi.org/10.3390/plants14233612

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