Identification of Hypoglycemic Glycolipids from Ipomoea murucoides by Affinity-Directed Fractionation, In Vitro, In Silico and Dynamic Light Scattering Analysis
Abstract
:1. Introduction
2. Results
2.1. LRESI-MS Study of the Methanolic Extract from the Flowers of Cazahuate
2.2. Affinity-Directed Fractionation with α-Glucosidases and the Methanolic Extract from Flowers of Cazahuate
2.3. Molecular Docking
2.4. Affinity-Directed Fractionation with Glucose-6-Phosphatase (G6Pase) and the Methanolic Extract from Cazahuate Flowers
2.5. In Vitro Hepatic Glucose-6-Phosphatase (G6Pase) Activity
2.6. Dynamic Light Scattering (DLS) with Yeast α-D-Glucosidase and the SEC Protocol
3. Discussion
3.1. Affinity-Directed Fractionation with α-Glucosidases and the Methanolic Extract from Flowers of Cazahuate
3.2. Molecular Docking
3.3. Affinity-Directed Fractionation with Glucose-6-Phosphatase (G6Pase) and the Methanolic Extract from Cazahuate Flowers
3.4. In Vitro Hepatic Glucose-6-Phosphatase (G6Pase) Activity
3.5. Dynamic Light Scattering (DLS) with Yeast α-D-Glucosidase and the SEC Protocol
4. Materials and Methods
4.1. General Experimental Procedure
4.2. Plant Material and Extracts
4.3. Affinity-Directed Fractionation
4.4. Glucose-6-Phosphatase Assay
4.5. Molecular Docking
4.6. Dynamic Light Scattering
4.7. Chromogenic Inhibition Assay of α-D-Glucosidase (EC 3.2.1.20) from S. cerevisiae
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Compound | Formula /MW | MAL12 | MGAM | ||
---|---|---|---|---|---|
Theoretical Ki | Hydrogen Bond | Theoretical Ki | Hydrogen Bond | ||
Murucoidin XIV | C57H100O20/1104 | 2.86 mM | ASN241 HIS279 GLN322 | 12.1 mM | GLY1588 |
Pescaprein V | C57H100O20/1104 | 1.23 mM | HIS279 | 346 mM | GLN1254 |
Pescaprein VI | C57H100O20/1104 | 304 mM | HIS279 | 118 mM | GLN1254 TRP1369 GLN1372 ARG1377 |
Murucoidin III | C56H96O24/1152 | 2.4 mM | HIS279 | 677 mM | ARG1377 THR1586 |
Stoloniferin I | C56H96O24/1152 | 244 mM | HIS279 | 4.28 mM | SER1366 ASP1370 GLN1372 ARG1377 |
Acarbose | C25H43NO18/645 | 51.4 μM | HIS279 GLN322 GLU304 ARG312 | 35.7 μM | TYR1251 GLN1372 ARG1377 GLN1561 GLY1588 |
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Rosas-Ramírez, D.; Arreguín-Espinosa, R.; Escandón-Rivera, S.; Andrade-Cetto, A.; Mata-Torres, G.; Pérez-Solís, R. Identification of Hypoglycemic Glycolipids from Ipomoea murucoides by Affinity-Directed Fractionation, In Vitro, In Silico and Dynamic Light Scattering Analysis. Plants 2024, 13, 644. https://doi.org/10.3390/plants13050644
Rosas-Ramírez D, Arreguín-Espinosa R, Escandón-Rivera S, Andrade-Cetto A, Mata-Torres G, Pérez-Solís R. Identification of Hypoglycemic Glycolipids from Ipomoea murucoides by Affinity-Directed Fractionation, In Vitro, In Silico and Dynamic Light Scattering Analysis. Plants. 2024; 13(5):644. https://doi.org/10.3390/plants13050644
Chicago/Turabian StyleRosas-Ramírez, Daniel, Roberto Arreguín-Espinosa, Sonia Escandón-Rivera, Adolfo Andrade-Cetto, Gerardo Mata-Torres, and Ricardo Pérez-Solís. 2024. "Identification of Hypoglycemic Glycolipids from Ipomoea murucoides by Affinity-Directed Fractionation, In Vitro, In Silico and Dynamic Light Scattering Analysis" Plants 13, no. 5: 644. https://doi.org/10.3390/plants13050644