Effects of Grazing and Leaf Spot Disease on the Structure and Diversity of Phyllosphere Microbiome Communities in Leymus chinensis

Qian, Yani; Jin, Yuanyuan; Han, Xinyao; Malik, Kamran; Li, Chunjie; Yu, Binhua

doi:10.3390/plants13152128

Open AccessArticle

Effects of Grazing and Leaf Spot Disease on the Structure and Diversity of Phyllosphere Microbiome Communities in Leymus chinensis

by

Yani Qian

¹,

Yuanyuan Jin

^2,3,4,5,6,

Xinyao Han

¹,

Kamran Malik

^2,3,4,5,6

,

Chunjie Li

^{1,2,3,4,5,6,*}

and

Binhua Yu

^2,3,4,5,6,*

¹

Grassland Research Center of National Forestry and Grassland Administration, Chinese Academy of Forestry, Beijing 100091, China

²

State Key Laboratory of Herbage Improvement and Grassland Agro-Ecosystems, Lanzhou University, Lanzhou 730020, China

³

College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 730020, China

⁴

Engineering Research Center of Grassland Industry, Ministry of Education, Lanzhou University, Lanzhou 730020, China

⁵

Key Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture and Rural Affairs, Lanzhou University, Lanzhou 730020, China

⁶

Gansu Tech Innovation Center of Western China Grassland Industry, Lanzhou University, Lanzhou 730020, China

^*

Authors to whom correspondence should be addressed.

Plants 2024, 13(15), 2128; https://doi.org/10.3390/plants13152128

Submission received: 5 June 2024 / Revised: 25 July 2024 / Accepted: 29 July 2024 / Published: 1 August 2024

(This article belongs to the Special Issue Genetic and Biological Diversity of Plants)

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Abstract

Leymus chinensis is a high-quality forage with wide distribution. Disease is an important factor affecting the yield and quality of L. chinensis. To investigate the effect of grazing on the phyllosphere microbiome community and leaf spot disease in L. chinensis, high-throughput sequencing technology was used to study the differences in the composition and structure of the phyllosphere fungal and bacterial communities of healthy and diseased leaves under different grazing intensities. The results showed that grazing significantly reduced leaf spot disease incidence and severity. There were significant differences in the phyllosphere microbiome composition between healthy and diseased leaves, and interestingly, diseased leaves showed more complex microbial activity. Grazing altered the relative abundance of micro-organisms and affected microbial dispersal and colonization either directly through behavior or indirectly by altering plant community structure. In this study, we found that the phyllosphere microbiome responded strongly to pathogen infection, and that plants recruited beneficial microbes to protect themselves after disease development. Grazing could regulate microbial community composition and structure, either directly or indirectly, and plays a crucial role in maintaining the health of L. chinensis.

Keywords: cattle grazing; plant community; fungi disease; phyllosphere microbiome; interaction

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MDPI and ACS Style

Qian, Y.; Jin, Y.; Han, X.; Malik, K.; Li, C.; Yu, B. Effects of Grazing and Leaf Spot Disease on the Structure and Diversity of Phyllosphere Microbiome Communities in Leymus chinensis. Plants 2024, 13, 2128. https://doi.org/10.3390/plants13152128

AMA Style

Qian Y, Jin Y, Han X, Malik K, Li C, Yu B. Effects of Grazing and Leaf Spot Disease on the Structure and Diversity of Phyllosphere Microbiome Communities in Leymus chinensis. Plants. 2024; 13(15):2128. https://doi.org/10.3390/plants13152128

Chicago/Turabian Style

Qian, Yani, Yuanyuan Jin, Xinyao Han, Kamran Malik, Chunjie Li, and Binhua Yu. 2024. "Effects of Grazing and Leaf Spot Disease on the Structure and Diversity of Phyllosphere Microbiome Communities in Leymus chinensis" Plants 13, no. 15: 2128. https://doi.org/10.3390/plants13152128

APA Style

Qian, Y., Jin, Y., Han, X., Malik, K., Li, C., & Yu, B. (2024). Effects of Grazing and Leaf Spot Disease on the Structure and Diversity of Phyllosphere Microbiome Communities in Leymus chinensis. Plants, 13(15), 2128. https://doi.org/10.3390/plants13152128

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

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Effects of Grazing and Leaf Spot Disease on the Structure and Diversity of Phyllosphere Microbiome Communities in Leymus chinensis

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