Characterization of the Isocitrate Dehydrogenase Gene Family and Their Response to Drought Stress in Maize
Abstract
:1. Introduction
2. Results
2.1. Identification of ZmIDH in Maize
2.2. Gene and Protein Structure Analysis of ZmIDHs
2.3. Colinearity and Duplication of ZmIDH Family Genes
2.4. Analysis of Promoter cis-Elements and the Protein Network of ZmIDH Genes
2.5. Expression Patterns of ZmIDHs
2.6. Candidate Gene Association Analysis of ZmIDHs Gene to Seed Suirvival Rate under Drought
3. Discussion
4. Conclusions
5. Materials and Methods
5.1. Identification of IDH Genes
5.2. Characterization of Gene Structure, Protein Tertiary Structure, and Motif Patterns
5.3. Chromosomal Distribution and Gene Duplication of ZmIDH Family Genes
5.4. Prediction and Correlation Analysis of IDH-Interacting Proteins
5.5. Expression Analysis of ZmIDH Genes
5.6. Candidate Gene Association Analysis and Haplotype Analysis
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Gene Name | Gene ID | Position | Protein Length (aa) | pI | MW | GRAVY | Subcellular Location |
---|---|---|---|---|---|---|---|
ZmIDH1.1 | Zm00001d028735 | Chr1:44718501–44719536 | 273 | 6.72 | 29,983.39 | −0.158 | Mitochondrion |
ZmIDH1.2 | Zm00001d040438 | Chr3:43449762–43455057 | 365 | 6.52 | 39,724.58 | −0.091 | Mitochondrion |
ZmIDH1.3 | Zm00001d050965 | Chr4:134060297–134069849 | 211 | 9.21 | 22,949.69 | −0.068 | Mitochondrion |
ZmIDH1.4 | Zm00001d017091 | Chr5:185084105–185089320 | 375 | 8.91 | 40,361.7 | −0.049 | Mitochondrion |
ZmIDH1.5 | Zm00001d008244 | Chr8:2452376–2459944 | 365 | 6.33 | 39,907.84 | −0.109 | Mitochondrion |
ZmIDH1.6 | Zm00001d025690 | Chr10:126319920–126323617 | 377 | 6.72 | 40,565.61 | −0.046 | Mitochondrion |
ZmIDH2.1 | Zm00001d003083 | Chr2:31519654–31526365 | 503 | 8.24 | 56,084.57 | −0.179 | Cytoplasmic |
ZmIDH2.2 | Zm00001d044021 | Chr3:216967820–216972096 | 412 | 6.57 | 46,097.73 | −0.231 | Cytoplasmic |
ZmIDH2.3 | Zm00001d039079 | Chr6:169685197–169688921 | 415 | 6.24 | 46,223.72 | −0.238 | Cytoplasmic |
ZmIDH2.4 | Zm00001d011487 | Chr8:152072966–152077705 | 412 | 6.11 | 46,196.77 | −0.245 | Cytoplasmic |
ZmIDH2.5 | Zm00001d046262 | Chr9:77157097–77159734 | 298 | 8.19 | 33,719.93 | −0.116 | Cytoplasmic; Chloroplast |
Gene ID | Gene Name | Polymorphic Number | MLM (p ≤ 0.05) | MLM (p ≤ 0.01) | MLM (p ≤ 0.001) | Max R2 (%) |
---|---|---|---|---|---|---|
Zm00001d028735 | ZmIDH1.1 | 14 | 3 | 1 | 0 | 1.956 |
Zm00001d040438 | ZmIDH1.2 | 30 | 1 | 0 | 0 | 1.611 |
Zm00001d050965 | ZmIDH1.3 | 23 | 0 | 0 | 0 | 1.029 |
Zm00001d017091 | ZmIDH1.4 | 32 | 4 | 4 | 2 | 6.706 |
Zm00001d008244 | ZmIDH1.5 | 27 | 4 | 1 | 0 | 2.094 |
Zm00001d025690 | ZmIDH1.6 | 55 | 3 | 1 | 0 | 2.329 |
Zm00001d003083 | ZmIDH2.1 | 20 | 0 | 0 | 0 | 1.024 |
Zm00001d044021 | ZmIDH2.2 | 42 | 1 | 1 | 0 | 2.372 |
Zm00001d039079 | ZmIDH2.3 | 66 | 10 | 1 | 0 | 2.032 |
Zm00001d011487 | ZmIDH2.4 | 28 | 1 | 0 | 0 | 1.833 |
Zm00001d046262 | ZmIDH2.5 | 52 | 1 | 0 | 0 | 1.213 |
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Wei, N.; Zhang, Z.; Yang, H.; Hu, D.; Wu, Y.; Xue, J.; Guo, D.; Xu, S. Characterization of the Isocitrate Dehydrogenase Gene Family and Their Response to Drought Stress in Maize. Plants 2023, 12, 3466. https://doi.org/10.3390/plants12193466
Wei N, Zhang Z, Yang H, Hu D, Wu Y, Xue J, Guo D, Xu S. Characterization of the Isocitrate Dehydrogenase Gene Family and Their Response to Drought Stress in Maize. Plants. 2023; 12(19):3466. https://doi.org/10.3390/plants12193466
Chicago/Turabian StyleWei, Ningning, Ziran Zhang, Haoxiang Yang, Die Hu, Ying Wu, Jiquan Xue, Dongwei Guo, and Shutu Xu. 2023. "Characterization of the Isocitrate Dehydrogenase Gene Family and Their Response to Drought Stress in Maize" Plants 12, no. 19: 3466. https://doi.org/10.3390/plants12193466