First Report of Hemp Fusarium Wilt Caused by Fusarium oxysporum in Croatia

Duvnjak, Tomislav; Vrandecic, Karolina; Sudaric, Aleksandra; Cosic, Jasenka; Siber, Tamara; Matosa Kocar, Maja

doi:10.3390/plants12183305

Open AccessArticle

First Report of Hemp Fusarium Wilt Caused by Fusarium oxysporum in Croatia

by

Tomislav Duvnjak

^1,*

,

Karolina Vrandecic

²

,

Aleksandra Sudaric

^1,3,

Jasenka Cosic

²,

Tamara Siber

²

and

Maja Matosa Kocar

¹

Department of Industrial Plants Breeding and Genetics, Agricultural Institute Osijek, 31000 Osijek, Croatia

²

Department of Phytomedicine, Faculty of Agrobiotechnical Sciences Osijek, Josip Juraj Strossmayer University of Osijek, 31000 Osijek, Croatia

³

Center of Excellence for Biodiversity and Molecular Plant Breeding, Faculty of Agriculture, University of Zagreb, Svetosimunska Cesta 25, 10000 Zagreb, Croatia

^*

Author to whom correspondence should be addressed.

Plants 2023, 12(18), 3305; https://doi.org/10.3390/plants12183305

Submission received: 3 August 2023 / Revised: 15 September 2023 / Accepted: 17 September 2023 / Published: 18 September 2023

(This article belongs to the Special Issue Breeding of Crop Disease-Resistant Cultivars)

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Abstract

Wilted hemp (Cannabis sativa L.) plants were observed in August 2019 in commercial fields around Osijek, Croatia. Plants and roots with disease symptoms were collected. The single-spored isolates produced septate cottony white to light pink aerial mycelium and purple undersurface on potato dextrose agar (PDA). Smooth and hyaline hyphae were branched and septate. Macroconidia were fusiform to sickle-shaped with foot-shaped basal cells, elongated apical cells and three to five septa. Sequencing of the internal transcribed spacer and the partial elongation factor 1-α gene identified the species as Fusarium oxysporum. Artificial infection fulfills Koch’s postulates, producing plants which show stunted growth and wilt symptoms similar to those observed in the commercial fields. Control seedlings remained symptomless and healthy. To the best of our knowledge, this is the first report of hemp Fusarium wilt causing F. oxysporum in Croatia. Considering that F. oxysporum has been reported in main field crops in Croatia, the presence of this pathogen could cause economically significant hemp production decreases, especially in humid and cold springs and susceptible varieties.

Keywords: hemp; Fusarium oxysporum; wilt; Croatia

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MDPI and ACS Style

Duvnjak, T.; Vrandecic, K.; Sudaric, A.; Cosic, J.; Siber, T.; Matosa Kocar, M. First Report of Hemp Fusarium Wilt Caused by Fusarium oxysporum in Croatia. Plants 2023, 12, 3305. https://doi.org/10.3390/plants12183305

AMA Style

Duvnjak T, Vrandecic K, Sudaric A, Cosic J, Siber T, Matosa Kocar M. First Report of Hemp Fusarium Wilt Caused by Fusarium oxysporum in Croatia. Plants. 2023; 12(18):3305. https://doi.org/10.3390/plants12183305

Chicago/Turabian Style

Duvnjak, Tomislav, Karolina Vrandecic, Aleksandra Sudaric, Jasenka Cosic, Tamara Siber, and Maja Matosa Kocar. 2023. "First Report of Hemp Fusarium Wilt Caused by Fusarium oxysporum in Croatia" Plants 12, no. 18: 3305. https://doi.org/10.3390/plants12183305

APA Style

Duvnjak, T., Vrandecic, K., Sudaric, A., Cosic, J., Siber, T., & Matosa Kocar, M. (2023). First Report of Hemp Fusarium Wilt Caused by Fusarium oxysporum in Croatia. Plants, 12(18), 3305. https://doi.org/10.3390/plants12183305

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

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First Report of Hemp Fusarium Wilt Caused by Fusarium oxysporum in Croatia

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