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Volume 12
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10.3390/plants12162953
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Article

Plant Growth Regulator- and Elicitor-Mediated Enhancement of Biomass and Andrographolide Production of Shoot Tip-Culture-Derived Plantlets of Andrographis paniculata (Burm.f.) Wall. (Hempedu Bumi)

by
Aicah Patuhai
1,
Puteri Edaroyati Megat Wahab
1,
Martini Mohammad Yusoff
1,
Yaser Hassan Dewir
2,
Ali Alsughayyir
3 and
Mansor Hakiman
1,4,*
1
Department of Crop Science, Faculty of Agriculture, Universiti Putra Malaysia (UPM), Serdang 43400, Selangor, Malaysia
2
Plant Production Department, College of Food and Agriculture Sciences, King Saud University, Riyadh 11451, Saudi Arabia
3
Department of Plant and Soil Sciences, Mississippi State University, 75 B.S. Hood Rd, Starkville, MS 39762, USA
4
Laboratory of Sustainable Resources Management, Institute of Tropical Forestry and Forest Products, Universiti Putra Malaysia (UPM), Serdang 43400, Selangor, Malaysia
*
Author to whom correspondence should be addressed.
Plants 2023, 12(16), 2953; https://doi.org/10.3390/plants12162953
Submission received: 19 June 2023 / Revised: 8 July 2023 / Accepted: 18 July 2023 / Published: 15 August 2023
(This article belongs to the Special Issue Production of Plant Secondary Metabolites Using Plant Tissue Culture and Bioreactor Culture Techniques)
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Abstract

Andrographis paniculata (Burm.f.) Wall. (Acanthaceae) is revered for its medicinal properties. In vitro culture of medicinal plants has assisted in improving both the quantity and quality of their yield. The current study investigated the effects of different surface sterilization treatments, plant growth regulators (PGRs), and elicitors on culture establishment and axillary shoot multiplication of A. paniculata. Subsequently, the production of andrographolide in the in vitro plantlets was evaluated using high-performance liquid chromatography (HPLC) analysis. The shoot-tip explant was successfully sterilized using 60% commercial bleach for 5 min of immersion with a 90% survival rate and 96.67% aseptic culture. The optimal PGR for shoot growth was 6-benzylaminopurine (BAP) at 17.76 µM, supplemented into Murashige and Skoog (MS) media, producing 23.57 ± 0.48 leaves, 7.33 ± 0.10 shoots, and a 3.06 ± 0.02 cm length of shoots. Subsequently, MS medium supplemented with 5 mg/L chitosan produced 26.07 ± 0.14 leaves, 8.33 ± 0.07 shoots, and a 3.63 ± 0.02 cm length of shoots. The highest andrographolide content was obtained using the plantlets harvested from 5 mg/L chitosan with 2463.03 ± 0.398 µg/mL compared to the control (without elicitation) with 256.73 ± 0.341 µg/mL (859.39% increase). The results imply that the protocol for the shoot-tip culture of A. paniculata was developed, and that elicitation enhanced the herbage yield and the production of andrographolide.
Keywords: chitosan; contamination; elicitation; plant growth regulators; salicylic acid; surface sterilization chitosan; contamination; elicitation; plant growth regulators; salicylic acid; surface sterilization

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MDPI and ACS Style

Patuhai, A.; Wahab, P.E.M.; Yusoff, M.M.; Dewir, Y.H.; Alsughayyir, A.; Hakiman, M. Plant Growth Regulator- and Elicitor-Mediated Enhancement of Biomass and Andrographolide Production of Shoot Tip-Culture-Derived Plantlets of Andrographis paniculata (Burm.f.) Wall. (Hempedu Bumi). Plants 2023, 12, 2953. https://doi.org/10.3390/plants12162953

AMA Style

Patuhai A, Wahab PEM, Yusoff MM, Dewir YH, Alsughayyir A, Hakiman M. Plant Growth Regulator- and Elicitor-Mediated Enhancement of Biomass and Andrographolide Production of Shoot Tip-Culture-Derived Plantlets of Andrographis paniculata (Burm.f.) Wall. (Hempedu Bumi). Plants. 2023; 12(16):2953. https://doi.org/10.3390/plants12162953

Chicago/Turabian Style

Patuhai, Aicah, Puteri Edaroyati Megat Wahab, Martini Mohammad Yusoff, Yaser Hassan Dewir, Ali Alsughayyir, and Mansor Hakiman. 2023. "Plant Growth Regulator- and Elicitor-Mediated Enhancement of Biomass and Andrographolide Production of Shoot Tip-Culture-Derived Plantlets of Andrographis paniculata (Burm.f.) Wall. (Hempedu Bumi)" Plants 12, no. 16: 2953. https://doi.org/10.3390/plants12162953

APA Style

Patuhai, A., Wahab, P. E. M., Yusoff, M. M., Dewir, Y. H., Alsughayyir, A., & Hakiman, M. (2023). Plant Growth Regulator- and Elicitor-Mediated Enhancement of Biomass and Andrographolide Production of Shoot Tip-Culture-Derived Plantlets of Andrographis paniculata (Burm.f.) Wall. (Hempedu Bumi). Plants, 12(16), 2953. https://doi.org/10.3390/plants12162953

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