Involvement of NO in V-ATPase Regulation in Cucumber Roots under Control and Cadmium Stress Conditions
Abstract
:1. Introduction
2. Results
3. Discussion
4. Materials and Methods
4.1. Plant Growing Conditions
4.2. Determination of NO, H2S and H2O2 Content
4.3. Determination of Phytohormone Content
4.4. Determination of Protein Level
4.5. Isolation of Tonoplast Fractions and Determination of V-ATPase Activities
4.6. Determination of Nitrate Reductase Activity
4.7. Total RNA Extraction, cDNA Synthesis and Gene Expression Analysis
4.8. Immunoblotting and Coomassie Staining
4.9. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Treatment | BeA Concentration (ng g−1 FW) | SA Concentration (ng g−1 FW) |
---|---|---|
Control (hν) | 67.1 ± 2.6 | 179.4 ± 6.2 |
SNP (hν) | 59.0 ± 3.4 | 190.7 ± 10.8 |
Cd | 74.0 ± 0.8 ^ | 252.9 ± 23.1 ^ |
Cd+L-NAME | 83.8 ± 4.1 * | 203.3 ± 3.3 |
Cd+WO42− | 71.6 ± 4.1 | 185.5 ± 5.9 * |
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Zboińska, M.; Janeczko, A.; Kabała, K. Involvement of NO in V-ATPase Regulation in Cucumber Roots under Control and Cadmium Stress Conditions. Plants 2023, 12, 2884. https://doi.org/10.3390/plants12152884
Zboińska M, Janeczko A, Kabała K. Involvement of NO in V-ATPase Regulation in Cucumber Roots under Control and Cadmium Stress Conditions. Plants. 2023; 12(15):2884. https://doi.org/10.3390/plants12152884
Chicago/Turabian StyleZboińska, Magdalena, Anna Janeczko, and Katarzyna Kabała. 2023. "Involvement of NO in V-ATPase Regulation in Cucumber Roots under Control and Cadmium Stress Conditions" Plants 12, no. 15: 2884. https://doi.org/10.3390/plants12152884