NADP-Dependent Malic Enzyme Genes in Sweet Pepper Fruits: Involvement in Ripening and Modulation by Nitric Oxide (NO)
Abstract
:1. Introduction
2. Results
2.1. Identification of the NADP-ME Genes in Pepper (Capsicum annuum L.): Sequence and Cis-Regulatory Elements
2.2. NADP-ME Proteins from Pepper: Sequence, Phylogenetic Analysis and Modeling
2.3. Fruit CaNADP-ME Genes: Expression during Ripening and Exogenous NO Treatment
2.4. Identification of the CaNADP-ME Isozymes in Pepper Fruits
3. Discussion
3.1. The Pepper Genome Contains Five CaNADP-ME Genes but Only Four Genes (CaNADP-ME2 to ME5) Are Expressed in Fruits
3.2. During Fruit Ripening, the Expression of CaNADP-ME2 and ME4 Is Downregulated, Whereas That of CaNADP-ME3 Is Upregulated, and Exogenous NO Gas Exerts a Negative Effect on CaNADP-ME3 and ME4
4. Materials and Methods
4.1. Identification of the NADP-ME Genes in Pepper (Capsicum annuum L.), Chromosomal Location and Synteny Analysis
4.2. Phylogenetic and Conserved Motif Analyses of NADP-ME Protein Sequences
4.3. Introns/Exons and Cis-Regulatory Elements Analysis of the CaNADP-ME Genes
4.4. Plant Material and Exogenous Nitric Oxide (NO) Gas Treatment
4.5. Library Preparation and RNA Sequencing
4.6. Fruit Extracts, Protein Assay, Protein Enrichment Using Ammonium–Sulfate [(NH4)2SO4] Fractionation and In-Gel Isozyme Profile of NADP-ME Activity
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Gene Name | Gene ID | Chr. | Genomic Location | Protein ID | Length (aa) | Da | Theoretical pI | Subcellular Localization |
---|---|---|---|---|---|---|---|---|
CaNADP-ME1 | 107861795 | 3 | 267357814–267362949 | XP_047266087.1 | 643 | 70,840 | 5.94 | Plastid |
CaNADP-ME2 | 107843507 | 5 | 158139423–158145651 | XP_016543302.1 | 578 | 64,245 | 5.80 | Cytosol |
CaNADP-ME3 | 107847755 | 8 | 26364857–26372640 | XP_016547705.1 | 618 | 68,468 | 6.57 | Plastid |
CaNADP-ME4 | 107842298 | 9 | 199489875–199498766 | XP_016541547.1 | 590 | 65,360 | 6.13 | Plastid |
CaNADP-ME5 | 107850438 | 12 | 97276100–97283614 | XP_016550447.1 | 644 | 70,709 | 6.28 | Plastid |
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Taboada, J.; González-Gordo, S.; Muñoz-Vargas, M.A.; Palma, J.M.; Corpas, F.J. NADP-Dependent Malic Enzyme Genes in Sweet Pepper Fruits: Involvement in Ripening and Modulation by Nitric Oxide (NO). Plants 2023, 12, 2353. https://doi.org/10.3390/plants12122353
Taboada J, González-Gordo S, Muñoz-Vargas MA, Palma JM, Corpas FJ. NADP-Dependent Malic Enzyme Genes in Sweet Pepper Fruits: Involvement in Ripening and Modulation by Nitric Oxide (NO). Plants. 2023; 12(12):2353. https://doi.org/10.3390/plants12122353
Chicago/Turabian StyleTaboada, Jorge, Salvador González-Gordo, María A. Muñoz-Vargas, José M. Palma, and Francisco J. Corpas. 2023. "NADP-Dependent Malic Enzyme Genes in Sweet Pepper Fruits: Involvement in Ripening and Modulation by Nitric Oxide (NO)" Plants 12, no. 12: 2353. https://doi.org/10.3390/plants12122353