Metagenomic Study of Fungal Microbial Communities in Two PDO Somontano Vineyards (Huesca, Spain): Effects of Age, Plant Genotype, and Initial Phytosanitary Status on the Priming and Selection of their Associated Microorganisms
Abstract
:1. Introduction
2. Results
2.1. Isolation of Endophytic Fungi from Grapevine Plants
2.2. NGS Analyses of Fungal Communities
2.2.1. Alpha-Diversity of Fungal Microbiome
2.2.2. Comparison between Vineyards: Beta-Diversity
2.3. Correlation between NGS and Culture-Dependent Methods
3. Discussion
3.1. Grapevine Inner Wood Microbiome
3.2. Comparison of Microbial Communities: Beta-Diversity
3.3. Comparative Microbial Diversity According to Methodology: NGS vs. Culture-Dependent Techniques
4. Materials and Methods
4.1. Grapevine Plots
4.2. Wood Samples
4.3. Isolation of Grapevine Endophytic Fungi
4.4. DNA Extraction and Sequencing
4.5. Bioinformatics Procedure
4.6. Statistical Data Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Taxonomy | Frequency | Fungal Guild |
---|---|---|
Cladosporium allicinum | 4601.6 | Pathotroph |
Epicoccum nigrum (V) | 5101 | Pathotroph–Saprotroph–Symbiotroph |
Stemphyliuym majusculum | 148.1 | Pathotroph–Saprotroph |
Cystofilobasidium macerans (V) | 138 | Saprotroph |
Seimatosporium vitis (V) | 1414.3 | Pathotroph |
Phaeomoniella chlamydospora (V) | 12,741.1 | Pathotroph |
Filobasidium stepposum (V) | 1028.8 | Saprotroph |
Knufia perforans | 308.9 | Pathotroph–Saprotroph |
Aureobasidium pullulans (V) | 683 | Pathotroph–Saprotroph–Symbiotroph |
Filobasidium magnum (V) | 69.2 | Saprotroph |
Rhinocladiella sp. (V) | 175 | Pathotroph |
Cladosporium grevilleae (V) | 358.1 | Saprotroph |
Cyphellophora sp. | 174.9 | Pathotroph–Saprotroph |
Cladosporium cladosporioides (V) | 8257.4 | Saprotroph |
Angustimassarina acerina (V) | 954.7 | Saprotroph |
Myrmecridium banksiae | 65.3 | Saprotroph |
Vishniacozyma victoriae (V) | 122.4 | Saprotroph |
Aspergillus undulatus | 643.2 | Pathotroph–Saprotroph |
Populocrescentia forlicesenensis | 1198.2 | Pathotroph–Saprotroph |
Ord. Malasseziales | 23.2 | Pathotroph–Saprotroph |
Acericola italica | 410.6 | Pathotroph–Saprotroph |
Sporobolomyces roseus | 128.8 | Pathotroph–Saprotroph |
Neoscytalidium sp. (V) | 43.8 | Pathotroph–Saprotroph |
Alternaria infectoria | 359.2 | Pathotroph–Saprotroph–Symbiotroph |
Neosetophoma lunariae (V) | 289.2 | Pathotroph |
Vishniacozyma carnescens (V) | 751.5 | Saprotroph |
Cladosporium exasperatum | 1392.3 | Saprotroph |
Alternaria alternata (V) | 2608.3 | Pathotroph–Saprotroph–Symbiotroph |
Cyphellophora oxyspora | 14.4 | Pathotroph–Saprotroph |
Knufia mediterranea | 221.7 | Pathotroph–Saprotroph |
Plot Name | “Clau” | “Almendros” |
---|---|---|
Var./Rootstock | “Cabernet Sauvignon” clone 170/SO4 | “Sauvignon Blanc”/376 and R140 |
Year Established | 2000 | 2015 |
Management/ Plantation frame | Cover crop, trellis formation system, and double cordon/3 × 1 m | Cover crop, trellis formation system, and double cordon/3 × 1.2 m |
Soil Type | Loam texture; calcisol (accumulation of calcium carbonate at a certain depth, basic pH) | Loam texture; calcisol |
Height (m.a.s.l) | 375 | 401 |
Temperature, Rainfall, and Climate | 14.23 °C, 486 mm, and continental Mediterranean climate | 13.00 °C, 486 mm, and continental Mediterranean climate |
Average Yield (last 3 years) | 6000 kg/ha | 9000 kg/ha |
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Langa-Lomba, N.; Grimplet, J.; Sánchez-Hernández, E.; Martín-Ramos, P.; Casanova-Gascón, J.; Julián-Lagunas, C.; González-García, V. Metagenomic Study of Fungal Microbial Communities in Two PDO Somontano Vineyards (Huesca, Spain): Effects of Age, Plant Genotype, and Initial Phytosanitary Status on the Priming and Selection of their Associated Microorganisms. Plants 2023, 12, 2251. https://doi.org/10.3390/plants12122251
Langa-Lomba N, Grimplet J, Sánchez-Hernández E, Martín-Ramos P, Casanova-Gascón J, Julián-Lagunas C, González-García V. Metagenomic Study of Fungal Microbial Communities in Two PDO Somontano Vineyards (Huesca, Spain): Effects of Age, Plant Genotype, and Initial Phytosanitary Status on the Priming and Selection of their Associated Microorganisms. Plants. 2023; 12(12):2251. https://doi.org/10.3390/plants12122251
Chicago/Turabian StyleLanga-Lomba, Natalia, Jerome Grimplet, Eva Sánchez-Hernández, Pablo Martín-Ramos, José Casanova-Gascón, Carmen Julián-Lagunas, and Vicente González-García. 2023. "Metagenomic Study of Fungal Microbial Communities in Two PDO Somontano Vineyards (Huesca, Spain): Effects of Age, Plant Genotype, and Initial Phytosanitary Status on the Priming and Selection of their Associated Microorganisms" Plants 12, no. 12: 2251. https://doi.org/10.3390/plants12122251