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Article

Desiccation Tolerance in Ramonda serbica Panc.: An Integrative Transcriptomic, Proteomic, Metabolite and Photosynthetic Study

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Institute of Molecular Genetics and Genetic Engineering, Laboratory for Plant Molecular Biology, University of Belgrade, Vojvode Stepe 444a, 11042 Belgrade, Serbia
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Department of Biomedical Sciences, University of Padova, Via Ugo Bassi 58/B, 35131 Padova, Italy
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Proteomics Center, University of Padova and Azienda Ospedaliera di Padova, Via G. Orus 2/B, 35129 Padova, Italy
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Biology Center of the Czech Academy of Sciences, Institute of Plant Molecular Biology, Department of Plant Biophysics and Biochemistry, Branišovska 31/1160, 370 05 Ceske Budejovice, Czech Republic
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Department of Agronomy, Food, Natural Resources, Animals and Environment, University of Padova, Viale dell’Università 16, 35020 Legnaro, Italy
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Institute for Multidisciplinary Research, Department of Life Science, University of Belgrade, Kneza Viseslava 1, 11000 Belgrade, Serbia
*
Authors to whom correspondence should be addressed.
Academic Editors: Jill M. Farrant and Mariam Awlia
Plants 2022, 11(9), 1199; https://doi.org/10.3390/plants11091199
Received: 25 March 2022 / Revised: 25 April 2022 / Accepted: 27 April 2022 / Published: 28 April 2022
(This article belongs to the Special Issue Desiccation/Salinity Tolerance and the Crosstalk Therein)
The resurrection plant Ramonda serbica Panc. survives long desiccation periods and fully recovers metabolic functions within one day upon watering. This study aimed to identify key candidates and pathways involved in desiccation tolerance in R. serbica. We combined differential transcriptomics and proteomics, phenolic and sugar analysis, FTIR analysis of the cell wall polymers, and detailed analysis of the photosynthetic electron transport (PET) chain. The proteomic analysis allowed the relative quantification of 1192 different protein groups, of which 408 were differentially abundant between hydrated (HL) and desiccated leaves (DL). Almost all differentially abundant proteins related to photosynthetic processes were less abundant, while chlorophyll fluorescence measurements implied shifting from linear PET to cyclic electron transport (CET). The levels of H2O2 scavenging enzymes, ascorbate-glutathione cycle components, catalases, peroxiredoxins, Fe-, and Mn superoxide dismutase (SOD) were reduced in DL. However, six germin-like proteins (GLPs), four Cu/ZnSOD isoforms, three polyphenol oxidases, and 22 late embryogenesis abundant proteins (LEAPs; mainly LEA4 and dehydrins), were desiccation-inducible. Desiccation provoked cell wall remodeling related to GLP-derived H2O2/HO activity and pectin demethylesterification. This comprehensive study contributes to understanding the role and regulation of the main metabolic pathways during desiccation aiming at crop drought tolerance improvement. View Full-Text
Keywords: cell wall remodeling; cyclic electron transport; drought; germin-like proteins; late embryogenesis abundant proteins; OJIP; pectin; polyphenol oxidase; resurrection plant; superoxide dismutase cell wall remodeling; cyclic electron transport; drought; germin-like proteins; late embryogenesis abundant proteins; OJIP; pectin; polyphenol oxidase; resurrection plant; superoxide dismutase
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MDPI and ACS Style

Vidović, M.; Battisti, I.; Pantelić, A.; Morina, F.; Arrigoni, G.; Masi, A.; Jovanović, S.V. Desiccation Tolerance in Ramonda serbica Panc.: An Integrative Transcriptomic, Proteomic, Metabolite and Photosynthetic Study. Plants 2022, 11, 1199. https://doi.org/10.3390/plants11091199

AMA Style

Vidović M, Battisti I, Pantelić A, Morina F, Arrigoni G, Masi A, Jovanović SV. Desiccation Tolerance in Ramonda serbica Panc.: An Integrative Transcriptomic, Proteomic, Metabolite and Photosynthetic Study. Plants. 2022; 11(9):1199. https://doi.org/10.3390/plants11091199

Chicago/Turabian Style

Vidović, Marija, Ilaria Battisti, Ana Pantelić, Filis Morina, Giorgio Arrigoni, Antonio Masi, and Sonja V. Jovanović. 2022. "Desiccation Tolerance in Ramonda serbica Panc.: An Integrative Transcriptomic, Proteomic, Metabolite and Photosynthetic Study" Plants 11, no. 9: 1199. https://doi.org/10.3390/plants11091199

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