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Article

Low Concentration of Anti-Auxin and Anti-Fungal Agent Accelerates the PLB Regeneration of Dendrobium okinawense under Green LED

1
The United Graduate School of Agricultural Sciences, Ehime University, Matsuyama 790-8556, Japan
2
Faculty of Agriculture, Kochi University, Kochi 783-8502, Japan
3
Graduate School of Agricultural Science, Kobe University, Kobe 657-8501, Japan
*
Author to whom correspondence should be addressed.
Academic Editor: Luigi Milella
Plants 2022, 11(8), 1082; https://doi.org/10.3390/plants11081082
Received: 2 March 2022 / Revised: 8 April 2022 / Accepted: 13 April 2022 / Published: 15 April 2022
(This article belongs to the Special Issue New Insight into Research in In Vitro Plants Propagation)
Dendrobium okinawense is an endangered epiphytic orchid, and there has been no scientific report so far on its propagation. Protocorm is a mass of cells, and protocorm-like bodies (PLBs) are lookalike protocorms produced by vegetative explants in vitro. Regeneration of PLBs is a widely used technique for orchid micropropagation. We used different light-emitting diodes (LEDs) for the PLB regeneration of D. okinawense. The number of PLBs and fresh weight were increased by 81.1% and 80.8%, respectively, under green LED over the white fluorescent (WF) light. We added different concentrations of PCIB (p-Chlorophenoxyisobutyric acid, an anti-auxin) and HMI (3-Hydroxy-5-methyl isoxazole, an anti-fungal agent) in culture media. The number of PLBs was increased in media having 0.01 mg/L of PCIB (35.9%) compared to control (no PCIB), whereas 19.3% increased in media having 0.01 mL/L of HMI compared to control (no HMI). Green LED in combination with 0.01 mg/L of PCIB significantly increased the number of PLBs (69.0%) compared to the WF–without PCIB combination. In LEDs-PCIB and LED-HMI combinations, HMI did not show better PLBs regeneration compared with PCIB. The results suggested that a combination of low concentrations of PCIB and green LED have the potential to accelerate PLB regeneration of D. okinawense. View Full-Text
Keywords: orchid; protocorm-like bodies; p-Chlorophenoxyisobutyric acid; 3-Hydroxy-5-methyl isoxazole; light-emitting diodes orchid; protocorm-like bodies; p-Chlorophenoxyisobutyric acid; 3-Hydroxy-5-methyl isoxazole; light-emitting diodes
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MDPI and ACS Style

Mehbub, H.; Shimasaki, K.; Mehraj, H. Low Concentration of Anti-Auxin and Anti-Fungal Agent Accelerates the PLB Regeneration of Dendrobium okinawense under Green LED. Plants 2022, 11, 1082. https://doi.org/10.3390/plants11081082

AMA Style

Mehbub H, Shimasaki K, Mehraj H. Low Concentration of Anti-Auxin and Anti-Fungal Agent Accelerates the PLB Regeneration of Dendrobium okinawense under Green LED. Plants. 2022; 11(8):1082. https://doi.org/10.3390/plants11081082

Chicago/Turabian Style

Mehbub, Hasan, Kazuhiko Shimasaki, and Hasan Mehraj. 2022. "Low Concentration of Anti-Auxin and Anti-Fungal Agent Accelerates the PLB Regeneration of Dendrobium okinawense under Green LED" Plants 11, no. 8: 1082. https://doi.org/10.3390/plants11081082

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