Bacterial-Assisted Extraction of Bioactive Compounds from Cauliflower
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Department of Biology and Biotechnology L. Spallanzani, University of Pavia, 27100 Pavia, Italy
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Department of Earth and Environmental Sciences, University of Pavia, 27100 Pavia, Italy
*
Author to whom correspondence should be addressed.
Academic Editors: Kwang-Hyun Baek and Petko Denev
Plants 2022, 11(6), 816; https://doi.org/10.3390/plants11060816
Received: 11 February 2022
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Revised: 11 March 2022
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Accepted: 17 March 2022
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Published: 18 March 2022
(This article belongs to the Special Issue Sustainable Recovery of Bio-Active Compounds from Fruit and Vegetable)
The market for nutraceutical molecules is growing at an impressive pace in all Western countries. A convenient source of bioactive compounds is found in vegetable waste products, and their re-use for the recovery of healthy biomolecules would increase the sustainability of the food production system. However, safe, cheap, and sustainable technologies should be applied for the recovery of these beneficial molecules, avoiding the use of toxic organic solvents or expensive equipment. The soil bacterium Bacillus subtilis is naturally endowed with several enzymes targeting complex vegetable polymers. In this work, a raw bacterial culture supernatant was used to assist in the extraction of bioactives using isothermal pressurization cycles. Besides a wild-type Bacillus subtilis strain, a new strain showing increased secretion of cellulases and xylanases, pivotal enzymes for the digestion of the plant cell wall, was also used. Results indicate that the recovery of compounds correlates with the amount of cellulolytic enzymes applied, demonstrating that the pretreatment with non-purified culture broth effectively promotes the release of bioactives from the vegetable matrix. Therefore, this approach is a valid and sustainable procedure for the recovery of bioactive compounds from food waste.
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Keywords:
Bacillus subtilis; cellulases; xylanases; bioactives; extraction; nutraceuticals; phytochemicals; sustainability; vegetable by-products
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
MDPI and ACS Style
Doria, E.; Buonocore, D.; Marra, A.; Bontà, V.; Gazzola, A.; Dossena, M.; Verri, M.; Calvio, C. Bacterial-Assisted Extraction of Bioactive Compounds from Cauliflower. Plants 2022, 11, 816. https://doi.org/10.3390/plants11060816
AMA Style
Doria E, Buonocore D, Marra A, Bontà V, Gazzola A, Dossena M, Verri M, Calvio C. Bacterial-Assisted Extraction of Bioactive Compounds from Cauliflower. Plants. 2022; 11(6):816. https://doi.org/10.3390/plants11060816
Chicago/Turabian StyleDoria, Enrico, Daniela Buonocore, Antonio Marra, Valeria Bontà, Andrea Gazzola, Maurizia Dossena, Manuela Verri, and Cinzia Calvio. 2022. "Bacterial-Assisted Extraction of Bioactive Compounds from Cauliflower" Plants 11, no. 6: 816. https://doi.org/10.3390/plants11060816
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