Antioxidant and Cytotoxic Activities of Kudzu Roots and Soy Molasses against Pediatric Tumors and Phytochemical Analysis of Isoflavones Using HPLC-DAD-ESI-HRMS

Aboushanab, Saied A.; Shevyrin, Vadim A.; Slesarev, Grigory P.; Melekhin, Vsevolod V.; Shcheglova, Anna V.; Makeev, Oleg G.; Kovaleva, Elena G.; Kim, Ki Hyun

doi:10.3390/plants11060741

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Antioxidant and Cytotoxic Activities of Kudzu Roots and Soy Molasses against Pediatric Tumors and Phytochemical Analysis of Isoflavones Using HPLC-DAD-ESI-HRMS

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Institute of Chemical Engineering, Ural Federal University Named after the First President of Russia B. N. Yeltsin, Mira 19, 620002 Yekaterinburg, Russia

Innovative Center of Chemical and Pharmaceutical Technologies, Laboratory of Organic Synthesis, Ural Federal University Named after the First President of Russia B. N. Yeltsin, Mira 19, 620002 Yekaterinburg, Russia

Department of Biology, Ural State Medical University, Repina 3, 620014 Yekaterinburg, Russia

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Department of Gene and Cell Therapy, Institute for Medical Cell Technologies, Karla Marksa 22a, 620026 Yekaterinburg, Russia

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School of Pharmacy, Sungkyunkwan University, Suwon 16419, Korea

Authors to whom correspondence should be addressed.

Academic Editor: Juei-Tang Cheng

Plants 2022, 11(6), 741; https://doi.org/10.3390/plants11060741

Received: 18 February 2022 / Revised: 7 March 2022 / Accepted: 7 March 2022 / Published: 10 March 2022

(This article belongs to the Topic Advances in Natural Products from Plants and Associated Microbes)

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Abstract

Pediatric solid tumors (PSTs) are life-threatening and can lead to high morbidity and mortality rates in children. Developing novel remedies to treat these tumors, such as glioblastoma multiforme and sarcomas, such as osteosarcoma, and rhabdomyosarcoma, is challenging, despite immense attempts with chemotherapeutic or radiotherapeutic interventions. Soy (Glycine max) and kudzu roots (KR) (Pueraria spp.) are well-known phytoestrogenic botanical sources that contain high amounts of naturally occurring isoflavones. In the present study, we investigated the antioxidant and cytotoxic effects of the extracts of KR and soy molasses (SM) against PSTs. The green extraction of isoflavones from KR and SM was performed using natural deep eutectic solvents. The extracts were subsequently analyzed by high-performance liquid chromatography (HPLC)-diode array detector (DAD) coupled with high-resolution (HR) mass spectrometry (MS), which identified 10 isoflavones in KR extracts and 3 isoflavones in the SM extracts. Antioxidant and cytotoxic activities of KR and SM extracts were assessed against glioblastoma multiforme (A-172), osteosarcoma (HOS), and rhabdomyosarcoma (Rd) cancer cell lines. The KR and SM extracts showed satisfactory cytotoxic effects (IC₅₀) against the cancer cell lines tested, particularly against Rd cancer cell lines, in a dose-dependent manner. Antioxidant activity was found to be significantly (p ≤ 0.05) higher in KR than in SM, which was consistent with the results of the cytotoxic activity observed with KR and SM extracts against glioblastoma and osteosarcoma cells. The total flavonoid content and antioxidant activities of the extracts were remarkably attributed to the isoflavone content in the KR and SM extracts. This study provides experimental evidence that HPLC-ESI-HRMS is a suitable analytical approach to identify isoflavones that exhibit potent antioxidant and anticancer potential against tumor cells, and that KR and SM, containing many isoflavones, can be a potential alternative for health care in the food and pharmaceutical industries. View Full-Text

Keywords: isoflavones; kudzu roots; soy molasses; HPLC-ESI-HRMS; cytotoxicity; glioblastoma; osteosarcoma; rhabdomyosarcoma

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Aboushanab, S.A.; Shevyrin, V.A.; Slesarev, G.P.; Melekhin, V.V.; Shcheglova, A.V.; Makeev, O.G.; Kovaleva, E.G.; Kim, K.H. Antioxidant and Cytotoxic Activities of Kudzu Roots and Soy Molasses against Pediatric Tumors and Phytochemical Analysis of Isoflavones Using HPLC-DAD-ESI-HRMS. Plants 2022, 11, 741. https://doi.org/10.3390/plants11060741

AMA Style

Aboushanab SA, Shevyrin VA, Slesarev GP, Melekhin VV, Shcheglova AV, Makeev OG, Kovaleva EG, Kim KH. Antioxidant and Cytotoxic Activities of Kudzu Roots and Soy Molasses against Pediatric Tumors and Phytochemical Analysis of Isoflavones Using HPLC-DAD-ESI-HRMS. Plants. 2022; 11(6):741. https://doi.org/10.3390/plants11060741

Chicago/Turabian Style

Aboushanab, Saied A., Vadim A. Shevyrin, Grigory P. Slesarev, Vsevolod V. Melekhin, Anna V. Shcheglova, Oleg G. Makeev, Elena G. Kovaleva, and Ki H. Kim. 2022. "Antioxidant and Cytotoxic Activities of Kudzu Roots and Soy Molasses against Pediatric Tumors and Phytochemical Analysis of Isoflavones Using HPLC-DAD-ESI-HRMS" Plants 11, no. 6: 741. https://doi.org/10.3390/plants11060741

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Antioxidant and Cytotoxic Activities of Kudzu Roots and Soy Molasses against Pediatric Tumors and Phytochemical Analysis of Isoflavones Using HPLC-DAD-ESI-HRMS

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