Next Article in Journal
Assessment of the Fertilization Capacity of the Aquaculture Sediment for Wheat Grass as Sustainable Alternative Use
Next Article in Special Issue
A Viral Suppressor of RNA Silencing May Be Targeting a Plant Defence Pathway Involving Fibrillarin
Previous Article in Journal
Ethnobotanical Survey of Local Flora Used for Medicinal Purposes among Indigenous People in Five Areas in Lagos State, Nigeria
Previous Article in Special Issue
Argonaute 2 Controls Antiviral Activity against Sweet Potato Mild Mottle Virus in Nicotiana benthamiana
 
 
Article

Transcriptomic Reprogramming, Alternative Splicing and RNA Methylation in Potato (Solanum tuberosum L.) Plants in Response to Potato Virus Y Infection

1
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, 117997 Moscow, Russia
2
Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 119991 Moscow, Russia
3
The James Hutton Institute, Invergowrie, Dundee DD2 5DA, UK
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editors: Fabrizio Cillo and Livia Stavolone
Plants 2022, 11(5), 635; https://doi.org/10.3390/plants11050635
Received: 27 December 2021 / Revised: 9 February 2022 / Accepted: 22 February 2022 / Published: 25 February 2022
(This article belongs to the Special Issue Plant Defense Responses against Viruses)
Plant-virus interactions are greatly influenced by environmental factors such as temperatures. In virus-infected plants, enhanced temperature is frequently associated with more severe symptoms and higher virus content. However, the mechanisms involved in controlling the temperature regulation of plant-virus interactions are poorly characterised. To elucidate these further, we analysed the responses of potato plants cv Chicago to infection by potato virus Y (PVY) at normal (22 °C) and elevated temperature (28 °C), the latter of which is known to significantly increase plant susceptibility to PVY. Using RNAseq analysis, we showed that single and combined PVY and heat-stress treatments caused dramatic changes in gene expression, affecting the transcription of both protein-coding and non-coding RNAs. Among the newly identified genes responsive to PVY infection, we found genes encoding enzymes involved in the catalysis of polyamine formation and poly ADP-ribosylation. We also identified a range of novel non-coding RNAs which were differentially produced in response to single or combined PVY and heat stress, that consisted of antisense RNAs and RNAs with miRNA binding sites. Finally, to gain more insights into the potential role of alternative splicing and epitranscriptomic RNA methylation during combined stress conditions, direct RNA nanopore sequencing was performed. Our findings offer insights for future studies of functional links between virus infections and transcriptome reprogramming, RNA methylation and alternative splicing. View Full-Text
Keywords: potato virus Y; heat stress; complex stress; direct RNA-seq; lncRNAs; PARylation potato virus Y; heat stress; complex stress; direct RNA-seq; lncRNAs; PARylation
Show Figures

Figure 1

MDPI and ACS Style

Glushkevich, A.; Spechenkova, N.; Fesenko, I.; Knyazev, A.; Samarskaya, V.; Kalinina, N.O.; Taliansky, M.; Love, A.J. Transcriptomic Reprogramming, Alternative Splicing and RNA Methylation in Potato (Solanum tuberosum L.) Plants in Response to Potato Virus Y Infection. Plants 2022, 11, 635. https://doi.org/10.3390/plants11050635

AMA Style

Glushkevich A, Spechenkova N, Fesenko I, Knyazev A, Samarskaya V, Kalinina NO, Taliansky M, Love AJ. Transcriptomic Reprogramming, Alternative Splicing and RNA Methylation in Potato (Solanum tuberosum L.) Plants in Response to Potato Virus Y Infection. Plants. 2022; 11(5):635. https://doi.org/10.3390/plants11050635

Chicago/Turabian Style

Glushkevich, Anna, Nadezhda Spechenkova, Igor Fesenko, Andrey Knyazev, Viktoriya Samarskaya, Natalia O. Kalinina, Michael Taliansky, and Andrew J. Love. 2022. "Transcriptomic Reprogramming, Alternative Splicing and RNA Methylation in Potato (Solanum tuberosum L.) Plants in Response to Potato Virus Y Infection" Plants 11, no. 5: 635. https://doi.org/10.3390/plants11050635

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop