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Article

A Temporary Immersion System Improves Regeneration of In Vitro Irradiated Recalcitrant Indica Rice (Oryza sativa L.) Embryogenic Calli

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Doctorado en Ciencias Naturales para el Desarrollo (DOCINADE), Instituto Tecnológico de Costa Rica, Universidad Nacional, Universidad Estatal a Distancia, Cartago P.O. Box 159-7050, Costa Rica
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Biotechnology Research Center, Biology School, Costa Rica Institute of Technology, Cartago P.O. Box 159-7050, Costa Rica
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Plant Biotechnology Laboratory, School of Biology, University of Costa Rica, San José P.O. Box 2060, Costa Rica
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Programa de Posgrado en Ciencias Agrícolas y Recursos Naturales (PPCARN), School of Agronomy, University of Costa Rica, San José P.O. Box 2060, Costa Rica
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Vitroflora Labs S.A., Alajuela 20701, Costa Rica
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Programa de Posgrado en Biología (PPB), School of Biology, University of Costa Rica, San José P.O. Box 2060, Costa Rica
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Gamma Irradiation Laboratory, School of Physics, Costa Rica Institute of Technology, Cartago P.O. Box 159-7050, Costa Rica
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Author to whom correspondence should be addressed.
Academic Editor: Iyyakkannu Sivanesan
Plants 2022, 11(3), 375; https://doi.org/10.3390/plants11030375
Received: 23 December 2021 / Revised: 25 January 2022 / Accepted: 26 January 2022 / Published: 29 January 2022
(This article belongs to the Special Issue Plant Tissue Culture II)
The development of gamma ray-mutated rice lines is a solution for introducing genetic variability in indica rice varieties already being used by farmers. In vitro gamma ray (60Co) mutagenesis reduces chimeras and allows for a faster selection of desirable traits but requires the optimization of the laboratory procedure. The objectives of the present work were sequencing of matK and rbcL, the in vitro establishment of recalcitrant rice embryogenic calli, the determination of their sensitivity to gamma radiation, and optimization of the generation procedure. All sequenced genes matched perfectly with previously reported matK and rbcL O. sativa genes. Embryogenic calli induction improved using MS medium containing 2 mg L−1 2,4-D, and regeneration was achieved with MS medium with 3 mg L−1 BA and 0.5 mg L−1 NAA. The optimized radiation condition was 60 Gy, (LD20 = 64 Gy) with 83% regeneration. An immersion system (RITA®, Saint-Mathieu-de-Tréviers, France) of either 60 or 120 s every 8 h allowed systematic and homogeneous total regeneration of the recalcitrant line. Other well-known recalcitrant cultivars, CR1821 and CR1113, also had improved regeneration in the immersion system. To our knowledge, this is the first study reporting the use of an immersion system to allow for the regeneration of gamma-ray mutants from recalcitrant indica rice materials. View Full-Text
Keywords: somatic embryogenesis; Cobalt-60; radiation-induced mutagenesis; temporary immersion systems (TIS) somatic embryogenesis; Cobalt-60; radiation-induced mutagenesis; temporary immersion systems (TIS)
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MDPI and ACS Style

Hernández-Soto, A.; Pérez, J.; Fait-Zúñiga, R.; Rojas-Vásquez, R.; Gatica-Arias, A.; Vargas-Segura, W.; Abdelnour-Esquivel, A. A Temporary Immersion System Improves Regeneration of In Vitro Irradiated Recalcitrant Indica Rice (Oryza sativa L.) Embryogenic Calli. Plants 2022, 11, 375. https://doi.org/10.3390/plants11030375

AMA Style

Hernández-Soto A, Pérez J, Fait-Zúñiga R, Rojas-Vásquez R, Gatica-Arias A, Vargas-Segura W, Abdelnour-Esquivel A. A Temporary Immersion System Improves Regeneration of In Vitro Irradiated Recalcitrant Indica Rice (Oryza sativa L.) Embryogenic Calli. Plants. 2022; 11(3):375. https://doi.org/10.3390/plants11030375

Chicago/Turabian Style

Hernández-Soto, Alejandro, Jason Pérez, Rebeca Fait-Zúñiga, Randall Rojas-Vásquez, Andrés Gatica-Arias, Walter Vargas-Segura, and Ana Abdelnour-Esquivel. 2022. "A Temporary Immersion System Improves Regeneration of In Vitro Irradiated Recalcitrant Indica Rice (Oryza sativa L.) Embryogenic Calli" Plants 11, no. 3: 375. https://doi.org/10.3390/plants11030375

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