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Article

Genome Mining and Gene Expression Reveal Maytansine Biosynthetic Genes from Endophytic Communities Living inside Gymnosporia heterophylla (Eckl. and Zeyh.) Loes. and the Relationship with the Plant Biosynthetic Gene, Friedelin Synthase

1
Technical Biochemistry, Department of Biochemical and Chemical Engineering, TU Dortmund University, 44227 Dortmund, Germany
2
Department of Chemistry and Chemical Biology, Institute of Environmental Research (INFU), TU Dortmund University, 44227 Dortmund, Germany
*
Author to whom correspondence should be addressed.
Academic Editors: Konstantin V. Kiselev, William Underwood and Olga A. Aleynova
Plants 2022, 11(3), 321; https://doi.org/10.3390/plants11030321
Received: 24 November 2021 / Revised: 9 January 2022 / Accepted: 22 January 2022 / Published: 25 January 2022
Even though maytansine was first discovered from Celastraceae plants, it was later proven to be an endophytic bacterial metabolite. However, a pure bacterial culture cannot synthesize maytansine. Therefore, an exclusive interaction between plant and endophytes is required for maytansine production. Unfortunately, our understanding of plant–endophyte interaction is minimal, and critical questions remain. For example: how do endophytes synthesize maytansine inside their plant host, and what is the impact of maytansine production in plant secondary metabolites? Our study aimed to address these questions. We selected Gymnosporia heterophylla as our model and used amino-hydroxybenzoic acid (AHBA) synthase and halogenase genes as biomarkers, as these two genes respond to biosynthesize maytansine. As a result, we found a consortium of seven endophytes involved in maytansine production in G. heterophylla, based on genome mining and gene expression experiments. Subsequently, we evaluated the friedelin synthase (FRS) gene’s expression level in response to biosynthesized 20-hydroxymaytenin in the plant. We found that the FRS expression level was elevated and linked with the expression of the maytansine biosynthetic genes. Thus, we achieved our goals and provided new evidence on endophyte–endophyte and plant–endophyte interactions, focusing on maytansine production and its impact on plant metabolite biosynthesis in G. heterophylla. View Full-Text
Keywords: AHBA synthase gene; halogenase gene; FRS gene; maytansine-producible endophytes; endophyte–endophyte interaction; plant–endophyte interaction AHBA synthase gene; halogenase gene; FRS gene; maytansine-producible endophytes; endophyte–endophyte interaction; plant–endophyte interaction
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MDPI and ACS Style

Pitakbut, T.; Spiteller, M.; Kayser, O. Genome Mining and Gene Expression Reveal Maytansine Biosynthetic Genes from Endophytic Communities Living inside Gymnosporia heterophylla (Eckl. and Zeyh.) Loes. and the Relationship with the Plant Biosynthetic Gene, Friedelin Synthase. Plants 2022, 11, 321. https://doi.org/10.3390/plants11030321

AMA Style

Pitakbut T, Spiteller M, Kayser O. Genome Mining and Gene Expression Reveal Maytansine Biosynthetic Genes from Endophytic Communities Living inside Gymnosporia heterophylla (Eckl. and Zeyh.) Loes. and the Relationship with the Plant Biosynthetic Gene, Friedelin Synthase. Plants. 2022; 11(3):321. https://doi.org/10.3390/plants11030321

Chicago/Turabian Style

Pitakbut, Thanet, Michael Spiteller, and Oliver Kayser. 2022. "Genome Mining and Gene Expression Reveal Maytansine Biosynthetic Genes from Endophytic Communities Living inside Gymnosporia heterophylla (Eckl. and Zeyh.) Loes. and the Relationship with the Plant Biosynthetic Gene, Friedelin Synthase" Plants 11, no. 3: 321. https://doi.org/10.3390/plants11030321

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