Steroidal Saponins with Plant Growth Stimulation Effects; Yucca schidigera as a Commercial Source
Abstract
:1. Introduction
2. Results
2.1. Stimulation Activity of Steroidal Saponins
2.2. Bio-Guided Fractionation of Yucca Schidigera
2.3. Characterization of YS-But-BBA and YS-But-BCA Fractions
2.4. Active Saponins in Yucca Schidigera Extract
3. Discussion
3.1. Stimulation Activity of Steroidal Saponins
3.2. Yucca schidigera as a Commercial Source of Growth Stimulating Saponins
4. Materials and Methods
4.1. General Experimental Procedures
4.2. Plant Material
4.3. Extraction and Isolation
4.4. UPLC-QToF/MSE Analysis
4.5. Etiolated Wheat Coleoptile Bioassay
4.6. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Saponin-Enriched Fraction | Compounds | Retention Time (min.) | [M–H]– | Fragmentation (m/z) | Molecular Formula [M–H]– | mDa |
---|---|---|---|---|---|---|
YS-But-BCA | 8 | 2.78 | 915.4567 | 753.4037, 591.3527 | C45H71O19 | −0.6 |
9 | 2.72 | 915.4586 | 753.4008, 591.3475 | C45H71O19 | −2.3 | |
10 | 2.30 | 913.4427 | 751.3886, 589.3322 | C45H69O19 | −0.6 | |
YS-But-BBA | 11 | 3.16 | 885.4481 | 753.4009, 591.3557 | C44H69O18 | −0.8 |
12 | 3.11 | 885.4537 | 723.3862, 591.3455 | C44H69O18 | −0.3 | |
13 | 2.64 | 883.4299 | 751.3875, 589.3236 | C44H67O18 | −2.8 |
1 H NMR Signal | HMBC Correlations | Methyl Assig. | Flowchart Information | ||
---|---|---|---|---|---|
4.77 and 4.80 (D14) | SP C25 DB | ||||
1.35 d | 42.9 | 54.4 (D6) | 109.5 (D5) | C-21 | SP C12 CO |
1.31 d | 42.6 | 54.4 (D6) | 109.5 (D5) | C-21 | SP C12 CO |
1.04 d (D2) | 26.2 (D1) | 27.6 | 65.2 (D1) | C-27 | SP C25 S |
0.67 d (D2) | 29.1 (D1) | 30.6 | 66.9 (D1) | C-27 | SP C25 R |
1.06 s (S2) | 54.2 | 55.8 | 213.1 (S1) | C-18 | SP C12 CO |
0.93 s | 30.4 (S10) | 36.0 | 41.9 (S9) | C-19 | H-5β |
Sugar Chain | Compounds | Retention Time (min) | [M + HCOO]– | Relative Abundance 1 |
---|---|---|---|---|
S1 | 8/9 | 2.76 | 961.4646/961.4651 | 8.5 |
10 | 2.30 | 959.4451 | 3.0 | |
S2 | 11/12 | 3.11 | 931.4529/931.4554 | 23.4 |
13 | 2.61 | 929.4365 | 8.9 |
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Durán, A.G.; Calle, J.M.; Butrón, D.; Pérez, A.J.; Macías, F.A.; Simonet, A.M. Steroidal Saponins with Plant Growth Stimulation Effects; Yucca schidigera as a Commercial Source. Plants 2022, 11, 3378. https://doi.org/10.3390/plants11233378
Durán AG, Calle JM, Butrón D, Pérez AJ, Macías FA, Simonet AM. Steroidal Saponins with Plant Growth Stimulation Effects; Yucca schidigera as a Commercial Source. Plants. 2022; 11(23):3378. https://doi.org/10.3390/plants11233378
Chicago/Turabian StyleDurán, Alexandra G., Juan M. Calle, Davinia Butrón, Andy J. Pérez, Francisco A. Macías, and Ana M. Simonet. 2022. "Steroidal Saponins with Plant Growth Stimulation Effects; Yucca schidigera as a Commercial Source" Plants 11, no. 23: 3378. https://doi.org/10.3390/plants11233378