Production of Nematicidal Pinosylvin Stilbenes in Cell Suspension Cultures of Pinus koraiensis by Fungal Elicitation
Abstract
:1. Introduction
2. Results
2.1. Content of Pinosylvin Stilbenes (DPME and PME) in Stem Bark in P. koraiensis
2.2. Induction and Proliferation of P. koraiensis Calli
2.3. Cell Suspension Culture
2.4. Enhanced Production of DPME and PME in Cell Suspension by Fungal Elicitor Treatment
2.5. DPME and PME Accumulation by Prolonged Culture of Calli
2.6. qPCR Analysis of PsSTS and PsPMT Genes
2.7. Nematicidal Activity of Crude Extracts from P. koraiensis Calli
3. Discussion
4. Materials and Methods
4.1. Callus Induction from Mature Zygotic Embryos of P. koraiensis
4.2. Callus Proliferation
4.3. Cell Suspension Culture
4.4. Preparation of Fungal Elicitors
4.5. Fungal Elicitor Treatment during Cell Suspension Culture of P. koraiensis
4.6. qRT-PCR Analysis in Fungal Elicitor-Treated Calli
4.7. GC–MS Analysis
4.8. Nematicidal Activity of Callus Extracts
4.9. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Kim, Y.R.; Han, J.Y.; Choi, Y.E. Production of Nematicidal Pinosylvin Stilbenes in Cell Suspension Cultures of Pinus koraiensis by Fungal Elicitation. Plants 2022, 11, 2933. https://doi.org/10.3390/plants11212933
Kim YR, Han JY, Choi YE. Production of Nematicidal Pinosylvin Stilbenes in Cell Suspension Cultures of Pinus koraiensis by Fungal Elicitation. Plants. 2022; 11(21):2933. https://doi.org/10.3390/plants11212933
Chicago/Turabian StyleKim, Yi Rae, Jung Yeon Han, and Yong Eui Choi. 2022. "Production of Nematicidal Pinosylvin Stilbenes in Cell Suspension Cultures of Pinus koraiensis by Fungal Elicitation" Plants 11, no. 21: 2933. https://doi.org/10.3390/plants11212933