Development and Characterization of 20 Genomic SSR Markers for Ornamental Cultivars of Weigela
Abstract
:1. Introduction
2. Results and Discussion
2.1. gSSR Marker Development
2.2. gSSR Characteristics
2.3. Applications with Weigela Cultivars
3. Materials and Methods
3.1. Plant Material and DNA Extraction
3.2. DNA Sequencing and Primer Design
3.3. Primer Screening and Optimization
3.4. gSSR Data Analysis
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Locus | GenBank Accession | Primer Sequences | Repeat Motif | Allele Size Range (bp) | Number of Alleles | Missing Data (%) |
---|---|---|---|---|---|---|
Wei002 | OM158066 | F: ACATCATCATCACTTGGGTGG | [GAAA]6 | 135–150 | 5 | 17.65 |
R: ACCAGCACTTTCAATCTTCC | ||||||
Wei003 | OM158067 | F: ACATTCCAAGGCCACAAATGC | [TC]9 | 148–181 | 8 | 0 |
R: GTGGTCCTTGAATGTGTTTCATACG | ||||||
Wei004 | OM158068 | F: TGCACCTCAAATGAGACACC | [CT]8 | 150–202 | 6 | 0 |
R: AGGAGGTGGAGGAGACAAGG | ||||||
Wei005 | OM158069 | F: TCGCTGATCGTTTGGAGTCC | [TCT]11 | 159–192 | 5 | 0 |
R: AGCAAAGTAACCCTAGACAGG | ||||||
Wei006 | OM158070 | F: TCCTTCAATGGAGAGAGCCC | [ATAG]8 | 156–175 | 4 | 0 |
R: CAGAACTTGAATTCATGTTGTTGCC | ||||||
Wei008 | OM158071 | F: GCGTGACAAATTGCTTACTTGG | [TATC]8 | 174–201 | 6 | 11.76 |
R: ACATGGTTCAACAGTCTCCC | ||||||
Wei011 | OM158072 | F: TTTCTCAGCAACCAAACCGC | [ACAT]6 | 237–249 | 4 | 0 |
R: AAAGCACAAGCACAGAAGGG | ||||||
Wei013 | OM158073 | F: CCTCAAGAAGAAAGCGCTGC | [GAA]10 | 280–297 | 5 | 0 |
R: ACCAGAGAAATGTGTAACGC | ||||||
Wei015 | OM158074 | F: GAGTGCCAATAGCCAAACCC | [AT]9 | 292–325 | 6 | 0 |
R: TCGAAAGGTGGACCAACTGG | ||||||
Wei017 | OM158075 | F: TCTTCTCATTTGGTGTAGCG | [AAT]11 | 201–217 | 4 | 5.88 |
R: CGCCACCAATTTGGGTAACG | ||||||
Wei026 | OM158076 | F: GTGATTGAGTTTGAGCCGCC | [TA]13 | 304–329 | 6 | 0 |
R: CATGCACCACACCTTCATGC | ||||||
Wei027 | OM158077 | F: GTAAACCAATCAGGCGCACC | [CAT]7 | 340–362 | 7 | 0 |
R: GCACAAGCAAAGAAGCCGG | ||||||
Wei028 | OM158078 | F: GAACCACAACTCAAGCTCCG | [TATG]7 | 308–340 | 6 | 0 |
R: TCCGACGATTATGCTCACCG | ||||||
Wei029 | OM158079 | F: CAATACGAGAAGTGACGCTACC | [TC]10 | 348–361 | 6 | 0 |
R: CCCTTGCATTAAGAGGGTGC | ||||||
Wei034 | OM158080 | F: TCATGCAGTCTAAGCCCACC | [CATA]6 | 383–432 | 6 | 0 |
R: GGTTACCGCGTGAAGTATGC | ||||||
Wei035 | OM158081 | F: TCCACTTCAACCTGAGCTGC | [AG]8 | 388–404 | 7 | 23.53 |
R: TTAGTGACCACATCGTGACG | ||||||
Wei039 | OM158082 | F: TTTCTGCCTAACCAAATCAGCC | [AG]9 | 149–197 | 7 | 8.82 |
R: CTCACACGTACCACTCTAGCC | ||||||
Wei040 | OM158083 | F: TTCAGTTGAAAGACCAACCG | [GA]8 | 171–221 | 5 | 0 |
R: CACATTGAGAGAGCAATAATTTCCC | ||||||
Wei043 | OM158084 | F: AAGAGTTCCGTCCATGCTGG | [AG]9 | 266–286 | 6 | 0 |
R: GCGAACAAGCTCAATTCCGG | ||||||
Wei044 | OM158085 | F: TCTAAAGCTCCATGGTCGGC | [AC]10 | 299–301 | 2 | 29.41 |
R: AGTGCCAATAGCCAATACCC |
Group a | Taxa | Nursery b | US Patent Number/Publication | Breeding Information c | Private Alleles (n) |
---|---|---|---|---|---|
1 | W. ‘ZR1’ Electric Love® | Pope’s Creekside Nursery | USPP30065 | W. ‘Tango’ × Open pollinated | 0 |
1 | W. ‘Minuet’ | Nature Hills | Svejda, 1982 d | W. ‘Purpurea’ × W. ‘Dropmore Pink’ | 2 |
1 | W. ‘Spring 2’ Stunner™ | Wayside Gardens | USPP30185 | Open Pollinated W. ‘Tango’ | 0 |
1 | W. ‘Tango’ | Pixie Gardens | Svejda, 1988 e | W. ‘Minuet’ × W. ‘Nana variegata’ | 1 |
2 | W. ‘Dark Horse’ | Nature Hills | USPP14381 | W. ‘Victoria’ g × W. ‘Foliis Purpureis’ | 1 |
2 | W. ‘TMWG16-04’ Towers of Flowers® Cherry | Wayside Gardens | USPP31915 | Open pollination W. florida ‘WG13003’ × W. florida ‘Alexandra’? h | 1 |
2 | W. ‘Velda’ Tuxedo™ | Wayside Gardens | USPP26842 | W. ‘Milk and Honey’ × W. florida ‘Alexandra’ | 3 |
3 | W. ‘Slingco 1’ Crimson Kisses® | Arbor Foundation | USPP23654 | W. ‘Evita’ × W. ‘Red Prince’ | 1 |
3 | W. ‘Slingco 2’ Date Night™ Maroon Swoon® | Pope’s Creekside Nursery | USPP26841 | Open pollinated W. ‘Red Prince’ | 2 |
3 | W. ‘Red Prince’ | Nature Hills | Weigle, 1991 f | W. ‘Vanicek’ × W. ISU41 | 3 |
N/A | W. ‘Verweig 3’ Minor Black | Wayside Gardens | N/A | unknown | 0 |
N/A | W. ‘Pink Poppet’ | Nature Hills | US 20030033647 P1 | W. florida ‘Venusta’ × W. hybrida ‘Eva Rathke’ | 6 |
N/A | W. ‘Kolmagira’ Rainbow Sensation™ | Nature Hills | USPP20384 | Cross-pollination of unnamed seedling | 2 |
N/A | W. ‘Bokrashine’ Shining Sensation™ | Nature Hills | N/A | unknown | 2 |
N/A | W. ‘Bokrasopin’ Sonic Bloom® Pink | Proven Winners | USPP24572 | Open Pollinated W. hybrida 93118 | 1 |
N/A | W. ‘Bokraspiwi’ Spilled Wine® | Pope’s Creekside Nursery | USPP23781 | Open Pollinated W. hybrida 93115 | 2 |
N/A | W. ‘Suzanne’ | Nature Hills | N/A | unknown | 5 |
N/A | W. ‘TMWG16-02’ Towers of Flowers® Apple Blossom | Wayside Gardens | USPP32237 | Open Pollinated W. florida ‘WG13001’ | 4 |
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Hamm, T.P.; Boggess, S.L.; Kandel, J.S.; Staton, M.E.; Huff, M.L.; Hadziabdic, D.; Shoemaker, D.; Adamczyk Jr., J.J.; Nowicki, M.; Trigiano, R.N. Development and Characterization of 20 Genomic SSR Markers for Ornamental Cultivars of Weigela. Plants 2022, 11, 1444. https://doi.org/10.3390/plants11111444
Hamm TP, Boggess SL, Kandel JS, Staton ME, Huff ML, Hadziabdic D, Shoemaker D, Adamczyk Jr. JJ, Nowicki M, Trigiano RN. Development and Characterization of 20 Genomic SSR Markers for Ornamental Cultivars of Weigela. Plants. 2022; 11(11):1444. https://doi.org/10.3390/plants11111444
Chicago/Turabian StyleHamm, Trinity P., Sarah L. Boggess, Jinita Sthapit Kandel, Margaret E. Staton, Matthew L. Huff, Denita Hadziabdic, DeWayne Shoemaker, John J. Adamczyk Jr., Marcin Nowicki, and Robert N. Trigiano. 2022. "Development and Characterization of 20 Genomic SSR Markers for Ornamental Cultivars of Weigela" Plants 11, no. 11: 1444. https://doi.org/10.3390/plants11111444