Characterization and QTL Mapping of a Major Field Resistance Locus for Bacterial Blight in Rice
Abstract
:1. Introduction
2. Results
2.1. Evaluation of BB Resistance Using the RIL Population
2.2. Development of the Mapping Population and R-Gene Inheritance
2.3. Analysis of QTLs for BB Resistance
2.4. Analysis of the Genetic Segregation Ratio of the Resistance Gene
2.5. Candidate Genes and Sequencing Analysis
3. Discussion
4. Materials and Methods
4.1. Plant Material and Field Design
4.2. Bacterial Strains and Plant Inoculations
4.3. DNA Extraction and PCR Analysis
4.4. Genotyping and Linkage Mapping
4.5. Analysis of Putative Candidate Genes
4.6. Haplotype Sequence Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Cultivar | K1 | K2 | K3 | K3a |
---|---|---|---|---|
Sindongjin | R | R | R | S |
JJ623 | R | R | R | MR |
HR27195 | R | R | R | R |
Cross | Number of Resistance | Number of Susceptible | Total Number | Segregation Ratio | X2 | p-Value |
JJ623/HR27195 | 29 | 61 | 90 | 1:3 | 2.50 | 0.113 |
QTL | Chr. | Position (cM) | Physical Position (Mbp) | Left Marker | Right Marker | LOD z | PVE y(%) | Add x | Dom w |
---|---|---|---|---|---|---|---|---|---|
qBBR11 | 11 | 242.7 | 18.49–18.69 | KJ11_067 | KJ11_068 | 5.47 | 24.5 | 1.26 | −0.09 |
Gene ID | Physical Position (bp) | Putative Function |
---|---|---|
Os11g0515500 | 18,485,308–18,490,843 | Similar to transport inhibitor response 1 protein |
Os11g0515700 | 18,493,370–18,494,393 | Nonprotein coding transcript |
Os11g0516000 | 18,503,539–18,507,701 | Similar to Serine palmitoyltransferase (Fragment) |
Os11g0516050 | 18,506,918–18,507,468 | Nonprotein coding transcript |
Os11g0516100 | 18,509,086–18,512,628 | Ribosomal protein L31 domain containing protein. |
Os11g0516400 | 18,520,372–18,521,065 | Hypothetical conserved gene |
Os11g0516800 | 18,531,653–18,541,501 | Hypothetical protein |
Os11g0517200 | 18,544,614–18,548,898 | Hypothetical conserved gene |
Os11g0517350 | 18,550,900–18,551,191 | Similar to Maturase K (Fragment) |
Os11g0517400 | 18,552,567–18,556,300 | Conserved hypothetical protein |
Os11g0517800 | 18,560,178–18,575,490 | eIF-2-alpha kinase GCN2 |
Os11g0518300 | 18,581,847–18,585,958 | Hypothetical protein |
Os11g0518600 | 18,608,152–18,611,496 | Conserved hypothetical protein |
Os11g0518801 | 18,622,623–18,622,684 | Nonprotein coding transcript |
Os11g0518900 | 18,636,476–18,650,782 | Conserved hypothetical protein |
Os11g0519100 | 18,655,353–18,658,368 | Similar to OSIGBa0132O24.2 protein |
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Park, J.-R.; Lee, C.-M.; Ji, H.; Baek, M.-K.; Seo, J.; Jeong, O.-Y.; Park, H.-S. Characterization and QTL Mapping of a Major Field Resistance Locus for Bacterial Blight in Rice. Plants 2022, 11, 1404. https://doi.org/10.3390/plants11111404
Park J-R, Lee C-M, Ji H, Baek M-K, Seo J, Jeong O-Y, Park H-S. Characterization and QTL Mapping of a Major Field Resistance Locus for Bacterial Blight in Rice. Plants. 2022; 11(11):1404. https://doi.org/10.3390/plants11111404
Chicago/Turabian StylePark, Jae-Ryoung, Chang-Min Lee, Hyeonso Ji, Man-Kee Baek, Jeonghwan Seo, O-Young Jeong, and Hyun-Su Park. 2022. "Characterization and QTL Mapping of a Major Field Resistance Locus for Bacterial Blight in Rice" Plants 11, no. 11: 1404. https://doi.org/10.3390/plants11111404