Study and Physical Mapping of the Species-Specific Tandem Repeat CS-237 Linked with 45S Ribosomal DNA Intergenic Spacer in Cannabis sativa L.
Abstract
:1. Introduction
2. Results
2.1. Search and Amplification of the CS-237 Repeat
2.2. Physical Mapping of the CS-237 Repeat with Other Cytogenetic Markers on C. sativa Chromosomes
2.3. Molecular Search of the CS-237 in 45S rDNA
2.4. Additional Study of C. sativa IGS Variants
2.5. Bioinformatical Analysis of CS-237 and delCS-237 Repeats Localization in Chromosomes
3. Discussion
4. Conclusions
5. Materials and Methods
5.1. Plant Material and Bioinformatic Analysis
5.2. Chromosome Preparation
5.3. DNA Isolation
5.4. PCR Analysis
5.5. DNA Probes and Fluorescent In Situ Hybridization (FISH)
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Alexandrov, O.S.; Romanov, D.V.; Divashuk, M.G.; Razumova, O.V.; Ulyanov, D.S.; Karlov, G.I. Study and Physical Mapping of the Species-Specific Tandem Repeat CS-237 Linked with 45S Ribosomal DNA Intergenic Spacer in Cannabis sativa L. Plants 2022, 11, 1396. https://doi.org/10.3390/plants11111396
Alexandrov OS, Romanov DV, Divashuk MG, Razumova OV, Ulyanov DS, Karlov GI. Study and Physical Mapping of the Species-Specific Tandem Repeat CS-237 Linked with 45S Ribosomal DNA Intergenic Spacer in Cannabis sativa L. Plants. 2022; 11(11):1396. https://doi.org/10.3390/plants11111396
Chicago/Turabian StyleAlexandrov, Oleg S., Dmitry V. Romanov, Mikhail G. Divashuk, Olga V. Razumova, Daniil S. Ulyanov, and Gennady I. Karlov. 2022. "Study and Physical Mapping of the Species-Specific Tandem Repeat CS-237 Linked with 45S Ribosomal DNA Intergenic Spacer in Cannabis sativa L." Plants 11, no. 11: 1396. https://doi.org/10.3390/plants11111396