In Vitro Production and Exudation of 20-Hydroxymaytenin from Gymnosporia heterophylla (Eckl. and Zeyh.) Loes. Cell Culture
1
Technical Biochemistry, Department of Biochemical and Chemical Engineering, TU Dortmund University, 44227 Dortmund, Germany
2
Institute of Environmental Research (INFU), Department of Chemistry and Chemical Biology, TU Dortmund University, 44227 Dortmund, Germany
*
Author to whom correspondence should be addressed.
Academic Editor: Iyyakkannu Sivanesan
Plants 2021, 10(8), 1493; https://doi.org/10.3390/plants10081493
Received: 4 May 2021
/
Revised: 9 July 2021
/
Accepted: 10 July 2021
/
Published: 21 July 2021
(This article belongs to the Special Issue Plant Tissue Culture II)
The metabolite 20-Hydroxymaytenin (20-HM) is a member of the quinone-methide pentacyclic triterpenoids (QMTs) group. This metabolite group is present only in Celastraceae plants, and it has shown various biological activities from antioxidant to anticancer properties. However, most QMTs metabolites including 20-HM cannot be synthesized in a laboratory. Therefore, we optimized a plant tissue culture protocol and examined the potential of Gymnosporia heterophylla (synonym. Maytenus heterophylla) to produce 20-HM in an in vitro experiment. For the first time, we reported the optimum callus induction medium with a high percentage success rate of 82% from the combination of 1 mg/L indole-3-butyric acid and 5 mg/L naphthalene acetic acid. Later, our cell suspension culture cultivated in the optimum medium provided approximately 0.35 mg/g fresh weight of 20-HM. This concentration is roughly 87.5 times higher than a concentration of 20-HM presenting in Elaeodendron croceum (Celastraceae) leaves. In addition, we also found that 20-HM presented in a cultivation medium, suggesting that G. heterophylla cells secreted 20-HM as an exudate in our experiment. Noticeably, 20-HM was missing when Penicillium cf. olsonii occurred in the medium. These findings hint at an antifungal property of 20-HM.
View Full-Text
▼
Show Figures
Figure 1
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited
- Supplementary File 1:
ZIP-Document (ZIP, 1319 KiB)
MDPI and ACS Style
Pitakbut, T.; Spiteller, M.; Kayser, O. In Vitro Production and Exudation of 20-Hydroxymaytenin from Gymnosporia heterophylla (Eckl. and Zeyh.) Loes. Cell Culture. Plants 2021, 10, 1493. https://doi.org/10.3390/plants10081493
AMA Style
Pitakbut T, Spiteller M, Kayser O. In Vitro Production and Exudation of 20-Hydroxymaytenin from Gymnosporia heterophylla (Eckl. and Zeyh.) Loes. Cell Culture. Plants. 2021; 10(8):1493. https://doi.org/10.3390/plants10081493
Chicago/Turabian StylePitakbut, Thanet, Michael Spiteller, and Oliver Kayser. 2021. "In Vitro Production and Exudation of 20-Hydroxymaytenin from Gymnosporia heterophylla (Eckl. and Zeyh.) Loes. Cell Culture" Plants 10, no. 8: 1493. https://doi.org/10.3390/plants10081493
Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.
