Genome-Wide Mapping of Histone H3 Lysine 4 Trimethylation (H3K4me3) and Its Involvement in Fatty Acid Biosynthesis in Sunflower Developing Seeds
Abstract
:1. Introduction
2. Materials and Methods
2.1. Biological Material and Growth Condition
2.2. Isolation and Immunoprecipitation of Chromatin
2.3. Computational Analysis of Sequencing Data
2.3.1. Quality Analysis and Filtering
2.3.2. Mapping Sequences
2.3.3. Aligned Sequences Evaluation and Peak-Calling
2.3.4. ChIP Peaks Annotation and Functional Enrichment Analysis
2.3.5. Motif Analysis: WRI1 Binding Sequences
2.4. mRNA Preparation and cDNA Synthesis
2.5. Quantitative Real-Time PCR
2.6. Analysis of Fatty Acid Composition
2.7. Statistical Analysis
3. Results and Discussion
3.1. Computational Analysis of Sequencing Data
3.2. Distribution of H3K4m3 Histone Modification in the Sunflower Genome
3.3. Functional Enrichment Analysis
3.4. Functional Annotation of Genes Involved in Fatty Acid Biosynthesis
3.5. Fatty Acid Composition in Developing Sunflower Seeds
3.6. WRI1 Gene Expression in Developing Sunflower Seeds
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Arabidopsis | Sunflower | Fold Enrichment * | |||
---|---|---|---|---|---|
Gene ID | Gene Name | Gene ID | Gene Name | 13 DAF | 28 DAF |
AT3G24650 | ABI3 | HanXRQChr15g0468411 | VIV1 | 6.37 | 5.39 |
AT3g26790 | FUS3 | HanXRQChr15g0475761 | FUS3 | 5.9 | 6.32 |
ChIP-Seq Annotation | ||||
---|---|---|---|---|
Enzyme Activity | Arabidopsis Gene | Sunflower Homologous Gene | 13 DAA | 28 DAA |
LS (Lipoyl Synthase) | AT5G08415.1 | HanXRQChr05g0142151 | Yes | Yes |
HanXRQChr12g0358451 | Yes | Yes | ||
HanXRQChr17g0559041 | Yes | Yes | ||
LT (Lipoyl Transferase) | AT4G31050.1 | HanXRQChr09g0268371 | Yes | Yes |
HanXRQChr05g0141551 | No | Yes | ||
HanXRQChr02g0056731 | No | Yes | ||
MCMT (Malonyl-CoA: ACP Malonyltransferase) | AT2G30200.1 | HanXRQChr01g0012161 | No | Yes |
HanXRQChr01g0012131 | Yes | Yes | ||
KASI (Ketoacyl-ACP Synthase I) | At5g46290 | HanXRQChr17g0564321 | Yes | Yes |
HanXRQChr04g0118391 | Yes | Yes | ||
HanXRQChr13g0415471 | Yes | Yes | ||
HanXRQChr17g0535001 | No | Yes | ||
HanXRQChr01g0020441 | No | Yes | ||
HanXRQChr01g0019841 | No | Yes | ||
KASII (Ketoacyl-ACP Synthase II) | At1g74960 | HanXRQChr09g0237851 | Yes | Yes |
HanXRQChr15g0497191 | Yes | Yes | ||
HanXRQChr06g0178221 | No | No | ||
HanXRQChr16g0508321 | Yes | Yes | ||
KASIII (Ketoacyl-ACP Synthase III) | At1g62640 | HanXRQChr02g0049441 | Yes | Yes |
HanXRQChr05g0148701 | Yes | Yes | ||
HanXRQChr17g0550351 | Yes | Yes | ||
KAR (Ketoacyl-ACP Reductase) | At1g24360 | |||
At1g62610 | ||||
At3g46170 | HanXRQChr17g0536131 | Yes | Yes | |
At3g55290 | HanXRQChr10g0318431 | Yes | Yes | |
At3g55310 | ||||
HAD (Hydroxyacyl-ACP Dehydrase) | At2g22230 | HanXRQChr03g0066951 | Yes | Yes |
At5g10160 | ||||
ER (Enoyl-ACP Reductase) | At2g05990 | HanXRQChr02g0056711 | No | Yes |
HanXRQChr17g0564011 | Yes | Yes | ||
HACPS (Holo-ACP Synthase) | At3g11470 | HanXRQChr08g0212801 | Yes | Yes |
HanXRQChr07g0194021 | No | Yes | ||
SAD (Stearoyl-ACP Desaturase) | At1g43800 | HanXRQChr01g0023271 | ||
At2g43710 | ||||
At3g02610 | ||||
At3g02620 | Yes | Yes | ||
At3g02630 | HanXRQChr11g0343101 | Yes | Yes | |
At5g16230 | ||||
At5g16240 | ||||
FATA (Acyl-ACP Thioesterase A) | At3g25110 | HanXRQChr01g0015251 | Yes | Yes |
FATB (Acyl-ACP Thioesterase B) | At1g08510 | HanXRQChr09g0240511 | Yes | Yes |
HanXRQChr06g0180041 | Yes | Yes | ||
HanXRQChr05g0138201 | No | Yes | ||
HanXRQChr10g0311291 | Yes | Yes | ||
HanXRQChr17g0553141 | No | Yes | ||
HanXRQChr10g0311351 | No | No | ||
HanXRQChr10g0311341 | No | No | ||
HanXRQChr10g0311311 | No | No | ||
HanXRQChr10g0311301 | No | No | ||
HanXRQChr01g0013271 | Yes | Yes | ||
HanXRQChr03g0062101 | No | Yes | ||
HanXRQChr07g0197741 | No | No |
13 DAA | 28 DAA | |
---|---|---|
16:0 | 12.18 ± 2.64 | 4.74 ± 0.25 * |
18:0 | 7.57 ± 0.56 | 2.83 ± 0.53 * |
18:1Δ9 | 53.65 ± 13.23 | 71.92 ± 1.27 |
18:2Δ9Δ12 | 25.72 ± 10.01 | 20.13 ± 1.31 |
20:0 | 0.55 ± 0.08 | 0.13 ± 0.01 * |
mg fatty acids/mg seed | 0.020 ± 0.003 | 0.125 ± 0.007 * |
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Moreno-Pérez, A.J.; Santos-Pereira, J.M.; Martins-Noguerol, R.; DeAndrés-Gil, C.; Troncoso-Ponce, M.A.; Venegas-Calerón, M.; Sánchez, R.; Garcés, R.; Salas, J.J.; Tena, J.J.; et al. Genome-Wide Mapping of Histone H3 Lysine 4 Trimethylation (H3K4me3) and Its Involvement in Fatty Acid Biosynthesis in Sunflower Developing Seeds. Plants 2021, 10, 706. https://doi.org/10.3390/plants10040706
Moreno-Pérez AJ, Santos-Pereira JM, Martins-Noguerol R, DeAndrés-Gil C, Troncoso-Ponce MA, Venegas-Calerón M, Sánchez R, Garcés R, Salas JJ, Tena JJ, et al. Genome-Wide Mapping of Histone H3 Lysine 4 Trimethylation (H3K4me3) and Its Involvement in Fatty Acid Biosynthesis in Sunflower Developing Seeds. Plants. 2021; 10(4):706. https://doi.org/10.3390/plants10040706
Chicago/Turabian StyleMoreno-Pérez, Antonio J., José M. Santos-Pereira, Raquel Martins-Noguerol, Cristina DeAndrés-Gil, M. Adrián Troncoso-Ponce, Mónica Venegas-Calerón, Rosario Sánchez, Rafael Garcés, Joaquín J. Salas, Juan J. Tena, and et al. 2021. "Genome-Wide Mapping of Histone H3 Lysine 4 Trimethylation (H3K4me3) and Its Involvement in Fatty Acid Biosynthesis in Sunflower Developing Seeds" Plants 10, no. 4: 706. https://doi.org/10.3390/plants10040706