Drought Intensity-Responsive Salicylic Acid and Abscisic Acid Crosstalk with the Sugar Signaling and Metabolic Pathway in Brassica napus
Abstract
:1. Introduction
2. Results
2.1. Leaf Water Status and Physiological Parameters
2.2. Endogenous SA and ABA Status
2.3. ABA, SA Synthesis, and Signaling Genes Expression
2.4. Assimilation of the Newly Fixed 13C into Soluble Sugar
2.5. Soluble Sugar and Starch Content
2.6. Sugar Metabolism- and Starch Degradation-Related Gene Expression
2.7. Sucrose Transport and Its Contribution to Osmotic Adjustment
2.8. Correlation among Variables Measured Parameters, Hormones, and Their Regulated Genes Expression for the Early and Late Phases of Drought Stress
3. Discussion
3.1. Carbohydrate Metabolism in Response to Drought-Stress Intensity
3.2. SA-Mediated Acclimation during the Early Period of Drought Stress
3.3. ABA-Mediated Sugar Signaling and Drought Symptom Development during the Late Period
4. Materials and Methods
4.1. Plant Growth, Drought Treatment, and Leaf Water Parameter Measurement
4.2. CO2 Labeling
4.3. Collection of Phloem Exudate and Sucrose Measurement
4.4. Extraction, Fractionation, and Analysis of Carbohydrates
4.5. 13C Isotope Analysis of Carbohydrate Fractionations
4.6. Phytohormone Analysis
4.7. Isolation of Total RNA and Quantitative Real-Time Polymerase Chain Reaction (qRT-PCR)
4.8. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Physiological Parameters | Days after Treatment | ||||
---|---|---|---|---|---|
/Treatment | 0 | 3 | 6 | 10 | 14 |
Leaf osmotic potential (MPa) | |||||
Control | −0.105 a | −1.117 a | −1.178 ab | −1.211 ab | −1.177 ab |
Drought | −0.102 a | −1.265 ab | −1.399 b | −1.712 c | −1.950 d |
Relative water content (RWC, %) | |||||
Control | 86.33 ab | 87.32 a | 86.56 ab | 84.91 ab | 85.07 ab |
Drought | 86.56 ab | 82.79 ab | 76.38 bc | 72.64 c | 70.98 c |
Leaf biomass (DM, g plant−1) | |||||
Control | 8.05 f | 8.78 ef | 11.94 bc | 13.40 b | 16.01 a |
Drought | 8.03 f | 8.24 f | 10.53 cd | 9.59 def | 9.97 de |
Days after Treatment | 0 | 3 | 6 | 10 | 14 | |||||
---|---|---|---|---|---|---|---|---|---|---|
Treatment | Control | Drought | Control | Drought | Control | Drought | Control | Drought | Control | Drought |
SA (ng g−1 DW) | 3.65 e | 3.65 e | 4.39 cde | 6.24 b | 3.87 de | 9.54 a | 5.60 bc | 6.27 b | 5.11 bcd | 4.14 de |
ABA (ng g−1 DW) | 6.76 e | 6.76 e | 7.38 e | 21.91 d | 8.09 e | 38.86 c | 10.89 e | 83.64 b | 9.58 e | 97.30 a |
ABA/SA ratio | 1.86 c | 1.86 c | 1.71 c | 3.55 c | 2.10 c | 4.06 c | 2.01 c | 13.52 b | 1.92 c | 24.04 a |
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Park, S.-H.; Lee, B.-R.; La, V.H.; Mamun, M.A.; Bae, D.-W.; Kim, T.-H. Drought Intensity-Responsive Salicylic Acid and Abscisic Acid Crosstalk with the Sugar Signaling and Metabolic Pathway in Brassica napus. Plants 2021, 10, 610. https://doi.org/10.3390/plants10030610
Park S-H, Lee B-R, La VH, Mamun MA, Bae D-W, Kim T-H. Drought Intensity-Responsive Salicylic Acid and Abscisic Acid Crosstalk with the Sugar Signaling and Metabolic Pathway in Brassica napus. Plants. 2021; 10(3):610. https://doi.org/10.3390/plants10030610
Chicago/Turabian StylePark, Sang-Hyun, Bok-Rye Lee, Van Hien La, Md Al Mamun, Dong-Won Bae, and Tae-Hwan Kim. 2021. "Drought Intensity-Responsive Salicylic Acid and Abscisic Acid Crosstalk with the Sugar Signaling and Metabolic Pathway in Brassica napus" Plants 10, no. 3: 610. https://doi.org/10.3390/plants10030610