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Volume 10
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10.3390/plants10030609
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Article

Genetic Diversity of Symbiotic Green Algae of Paramecium bursaria Syngens Originating from Distant Geographical Locations

by
Magdalena Greczek-Stachura
1,
Patrycja Zagata Leśnicka
1,
Sebastian Tarcz
2,
Maria Rautian
3 and
Katarzyna Możdżeń
1,*
1
Institute of Biology, Pedagogical University of Krakow, Podchorążych 2, 30-084 Kraków, Poland
2
Institute of Systematics and Evolution of Animals, Polish Academy of Sciences, Sławkowska 17, 31-016 Krakow, Poland
3
Laboratory of Protistology and Experimental Zoology, Faculty of Biology and Soil Science, St. Petersburg State University, Universitetskaya nab. 7/9, 199034 Saint Petersburg, Russia
*
Author to whom correspondence should be addressed.
Plants 2021, 10(3), 609; https://doi.org/10.3390/plants10030609
Submission received: 31 December 2020 / Revised: 16 March 2021 / Accepted: 21 March 2021 / Published: 23 March 2021
(This article belongs to the Special Issue Molecular Biology of Plastids)
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Abstract

Paramecium bursaria (Ehrenberg 1831) is a ciliate species living in a symbiotic relationship with green algae. The aim of the study was to identify green algal symbionts of P. bursaria originating from distant geographical locations and to answer the question of whether the occurrence of endosymbiont taxa was correlated with a specific ciliate syngen (sexually separated sibling group). In a comparative analysis, we investigated 43 P. bursaria symbiont strains based on molecular features. Three DNA fragments were sequenced: two from the nuclear genomes—a fragment of the ITS1-5.8S rDNA-ITS2 region and a fragment of the gene encoding large subunit ribosomal RNA (28S rDNA), as well as a fragment of the plastid genome comprising the 3′rpl36-5′infA genes. The analysis of two ribosomal sequences showed the presence of 29 haplotypes (haplotype diversity Hd = 0.98736 for ITS1-5.8S rDNA-ITS2 and Hd = 0.908 for 28S rDNA) in the former two regions, and 36 haplotypes in the 3′rpl36-5′infA gene fragment (Hd = 0.984). The following symbiotic strains were identified: Chlorella vulgaris, Chlorella variabilis, Chlorella sorokiniana and Micractinium conductrix. We rejected the hypotheses concerning (i) the correlation between P. bursaria syngen and symbiotic species, and (ii) the relationship between symbiotic species and geographic distribution.
Keywords: Paramecium bursaria algal symbionts; chloroplast 3′rpl.36-5′infA genes; nuclear ITS1-5.8S rDNA-ITS2; 28S rDNA sequence Paramecium bursaria algal symbionts; chloroplast 3′rpl.36-5′infA genes; nuclear ITS1-5.8S rDNA-ITS2; 28S rDNA sequence

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MDPI and ACS Style

Greczek-Stachura, M.; Leśnicka, P.Z.; Tarcz, S.; Rautian, M.; Możdżeń, K. Genetic Diversity of Symbiotic Green Algae of Paramecium bursaria Syngens Originating from Distant Geographical Locations. Plants 2021, 10, 609. https://doi.org/10.3390/plants10030609

AMA Style

Greczek-Stachura M, Leśnicka PZ, Tarcz S, Rautian M, Możdżeń K. Genetic Diversity of Symbiotic Green Algae of Paramecium bursaria Syngens Originating from Distant Geographical Locations. Plants. 2021; 10(3):609. https://doi.org/10.3390/plants10030609

Chicago/Turabian Style

Greczek-Stachura, Magdalena, Patrycja Zagata Leśnicka, Sebastian Tarcz, Maria Rautian, and Katarzyna Możdżeń. 2021. "Genetic Diversity of Symbiotic Green Algae of Paramecium bursaria Syngens Originating from Distant Geographical Locations" Plants 10, no. 3: 609. https://doi.org/10.3390/plants10030609

APA Style

Greczek-Stachura, M., Leśnicka, P. Z., Tarcz, S., Rautian, M., & Możdżeń, K. (2021). Genetic Diversity of Symbiotic Green Algae of Paramecium bursaria Syngens Originating from Distant Geographical Locations. Plants, 10(3), 609. https://doi.org/10.3390/plants10030609

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