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Article

Mitochondrial mRNA Processing in the Chlorophyte Alga Pediastrum duplex and Streptophyte Alga Chara vulgaris Reveals an Evolutionary Branch in Mitochondrial mRNA Processing

1
Department of Natural Sciences, The University of Virginia’s College at Wise, 1 College Ave., Wise, VA 24293, USA
2
Department of Biological and Environmental Sciences, Georgia College and State University, Milledgeville, GA 31061, USA
*
Author to whom correspondence should be addressed.
These authors contributed equally to this project.
Academic Editor: Nunzia Scotti
Plants 2021, 10(3), 576; https://doi.org/10.3390/plants10030576
Received: 18 February 2021 / Revised: 12 March 2021 / Accepted: 13 March 2021 / Published: 18 March 2021
(This article belongs to the Special Issue Genetics, Genomics and Biotechnology of Plant Cytoplasmic Organelles)
Mitochondria carry the remnant of an ancestral bacterial chromosome and express those genes with a system separate and distinct from the nucleus. Mitochondrial genes are transcribed as poly-cistronic primary transcripts which are post-transcriptionally processed to create individual translationally competent mRNAs. Algae post-transcriptional processing has only been explored in Chlamydomonas reinhardtii (Class: Chlorophyceae) and the mature mRNAs are different than higher plants, having no 5′ UnTranslated Regions (UTRs), much shorter and more variable 3′ UTRs and polycytidylated mature mRNAs. In this study, we analyzed transcript termini using circular RT-PCR and PacBio Iso-Seq to survey the 3′ and 5′ UTRs and termini for two green algae, Pediastrum duplex (Class: Chlorophyceae) and Chara vulgaris (Class: Charophyceae). This enabled the comparison of processing in the chlorophyte and charophyte clades of green algae to determine if the differences in mitochondrial mRNA processing pre-date the invasion of land by embryophytes. We report that the 5′ mRNA termini and non-template 3′ termini additions in P. duplex resemble those of C. reinhardtii, suggesting a conservation of mRNA processing among the chlorophyceae. We also report that C. vulgaris mRNA UTRs are much longer than chlorophytic examples, lack polycytidylation, and are polyadenylated similar to embryophytes. This demonstrates that some mitochondrial mRNA processing events diverged with the split between chlorophytic and streptophytic algae. View Full-Text
Keywords: mitochondria; RNA processing; algal evolution; circular RNA; polycytidylation; PacBio Iso-Seq mitochondria; RNA processing; algal evolution; circular RNA; polycytidylation; PacBio Iso-Seq
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MDPI and ACS Style

Proulex, G.C.R.; Meade, M.J.; Manoylov, K.M.; Cahoon, A.B. Mitochondrial mRNA Processing in the Chlorophyte Alga Pediastrum duplex and Streptophyte Alga Chara vulgaris Reveals an Evolutionary Branch in Mitochondrial mRNA Processing. Plants 2021, 10, 576. https://doi.org/10.3390/plants10030576

AMA Style

Proulex GCR, Meade MJ, Manoylov KM, Cahoon AB. Mitochondrial mRNA Processing in the Chlorophyte Alga Pediastrum duplex and Streptophyte Alga Chara vulgaris Reveals an Evolutionary Branch in Mitochondrial mRNA Processing. Plants. 2021; 10(3):576. https://doi.org/10.3390/plants10030576

Chicago/Turabian Style

Proulex, Grayson C. R., Marcus J. Meade, Kalina M. Manoylov, and A. Bruce Cahoon. 2021. "Mitochondrial mRNA Processing in the Chlorophyte Alga Pediastrum duplex and Streptophyte Alga Chara vulgaris Reveals an Evolutionary Branch in Mitochondrial mRNA Processing" Plants 10, no. 3: 576. https://doi.org/10.3390/plants10030576

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