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Molecular Characterization of Three B-1,4-Endoglucanase Genes in Pratylenchus loosi and Functional Analysis of Pl-eng-2 Gene

1
Plant Protection Department, Faculty of Agricultural Sciences, University of Guilan, Rasht 4199613769, Iran
2
Istituto per la Protezione Sostenibile delle Piante (IPSP), Consiglio Nazionale delle Ricerche (CNR), S.S. Bari, Via G. Amendola 122/D, 70126 Bari, Italy
3
Department of Biotechnology, Faculty of Agricultural Sciences, University of Guilan, Rasht 4199613769, Iran
*
Author to whom correspondence should be addressed.
These authors contributed equally.
Academic Editor: Pablo Castillo
Plants 2021, 10(3), 568; https://doi.org/10.3390/plants10030568
Received: 10 February 2021 / Revised: 12 March 2021 / Accepted: 14 March 2021 / Published: 17 March 2021
(This article belongs to the Section Plant Protection and Biotic Interactions)
Pratylenchus loosi is an important root-lesion nematode that causes damage to tea plantations in Iran and all over the world. The present study reports on the characterization and evolution of three ß-1,4-endoglucanase genes: Pl-eng-2, Pl-eng-3 and Pl-eng-4. The gene structure of Pl-eng-2 was fully determined with the predicted signal peptide and devoid of the linker domain and carbohydrate-binding domain, while Pl-eng-3 and Pl-eng-4 were only partially sequenced. The transcription of Pl-eng-2 was localized in the secretory esophageal glands of all life stages, but it was upregulated in male and female stages. The exon/intron structures of Pl-eng-2, Pl-eng-3 and Pl-eng-4 confirmed that they resulted from gene duplication followed by sequence and gene structure diversification with loss of the linker domain and carbohydrate-binding domain during evolution. A phylogenetic analysis further confirmed that nematode endoglucanases resulted from the horizontal gene transfer of a bacterial gene, as Pl-eng-3 showed sister relationships with the CelB cellulase of Bacillus subtilis. Silencing Pl-eng-2 by in vitro RNA interference produced a 60% decrease of the transcript level. The reproductive ability of silenced P. loosi showed a 35% reduction of eggs and larval stages compared to untreated nematodes, suggesting that this gene is involved in the early steps of invasion. View Full-Text
Keywords: cellulase; evolution; gene duplication; intron; RNA interference; root-lesion nematode cellulase; evolution; gene duplication; intron; RNA interference; root-lesion nematode
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MDPI and ACS Style

Mirghasemi, N.; Fanelli, E.; Jamali, S.; Sohani, M.M.; De Luca, F. Molecular Characterization of Three B-1,4-Endoglucanase Genes in Pratylenchus loosi and Functional Analysis of Pl-eng-2 Gene. Plants 2021, 10, 568. https://doi.org/10.3390/plants10030568

AMA Style

Mirghasemi N, Fanelli E, Jamali S, Sohani MM, De Luca F. Molecular Characterization of Three B-1,4-Endoglucanase Genes in Pratylenchus loosi and Functional Analysis of Pl-eng-2 Gene. Plants. 2021; 10(3):568. https://doi.org/10.3390/plants10030568

Chicago/Turabian Style

Mirghasemi, Negin, Elena Fanelli, Salar Jamali, Mohammed M. Sohani, and Francesca De Luca. 2021. "Molecular Characterization of Three B-1,4-Endoglucanase Genes in Pratylenchus loosi and Functional Analysis of Pl-eng-2 Gene" Plants 10, no. 3: 568. https://doi.org/10.3390/plants10030568

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