Next Article in Journal
A Comparison of the Transcriptomes of Cowpeas in Response to Two Different Ionizing Radiations
Previous Article in Journal
Proceedings of the 7th Series of Seminars on Advances in Apomixis Research
Previous Article in Special Issue
Cytotoxicity of Ficus Crocata Extract on Cervical Cancer Cells and Protective Effect against Hydrogen Peroxide-Induced Oxidative Stress in HaCaT Non-Tumor Cells
Open AccessArticle

Different Cannabis sativa Extraction Methods Result in Different Biological Activities against a Colon Cancer Cell Line and Healthy Colon Cells

1
Faculty of Medicine, Institute of Biomedical Sciences, University of Maribor, Taborska ulica 8, SI-2000 Maribor, Slovenia
2
BioCore Institute, Nad izviri 8, SI-2204 Miklavž na Dravskem Polju, Slovenia
3
Department of Chemistry, Faculty of Medicine, University of Maribor, Taborska ulica 8, SI-2000 Maribor, Slovenia
4
Laboratory for Separation Processes and Product Design, Faculty of Chemistry and Chemical Engineering, University of Maribor, Smetanova ulica 17, SI-2000 Maribor, Slovenia
5
Department of Pharmacology, Faculty of Medicine, University of Maribor, Taborska ulica 8, SI-2000 Maribor, Slovenia
*
Authors to whom correspondence should be addressed.
Academic Editor: Antonella Smeriglio
Plants 2021, 10(3), 566; https://doi.org/10.3390/plants10030566
Received: 1 March 2021 / Revised: 10 March 2021 / Accepted: 12 March 2021 / Published: 17 March 2021
(This article belongs to the Special Issue Anticancer Compounds in Plants)
Cannabis sativa is one of the oldest medicinal plants used by humans, containing hundreds of bioactive compounds. The biological effects and interplay of these compounds are far from fully understood, although the plant’s therapeutic effects are beyond doubt. Extraction methods for these compounds are becoming an integral part of modern Cannabis-based medicine. Still, little is known about how different methods affect the final composition of Cannabis extracts and thus, their therapeutic effects. In this study, different extraction methods were tested, namely maceration, Soxhlet, ultrasound-assisted extraction (UAE), and supercritical CO2 extraction methods. The obtained extracts were evaluated for their cannabinoid content, antioxidant properties, and in vitro bioactivity on human colon cancer and healthy colon cells. Our data suggest that Cannabis extracts, when properly prepared, can significantly decrease cancer cell viability while protecting healthy cells from cytotoxic effects. However, post-processing of extracts poses a significant limitation in predicting therapeutic response based on the composition of the crude extract, as it affects not only the actual amounts of the respective cannabinoids but also their relative ratio to the primary extracts. These effects must be carefully considered in the future preparations of new therapeutic extracts. View Full-Text
Keywords: extractions; Cannabis sativa; LC/MS-MS; cannabinoids; antioxidant; total phenols; cancer extractions; Cannabis sativa; LC/MS-MS; cannabinoids; antioxidant; total phenols; cancer
Show Figures

Figure 1

MDPI and ACS Style

Rožanc, J.; Kotnik, P.; Milojević, M.; Gradišnik, L.; Knez Hrnčič, M.; Knez, Ž.; Maver, U. Different Cannabis sativa Extraction Methods Result in Different Biological Activities against a Colon Cancer Cell Line and Healthy Colon Cells. Plants 2021, 10, 566. https://doi.org/10.3390/plants10030566

AMA Style

Rožanc J, Kotnik P, Milojević M, Gradišnik L, Knez Hrnčič M, Knez Ž, Maver U. Different Cannabis sativa Extraction Methods Result in Different Biological Activities against a Colon Cancer Cell Line and Healthy Colon Cells. Plants. 2021; 10(3):566. https://doi.org/10.3390/plants10030566

Chicago/Turabian Style

Rožanc, Jan; Kotnik, Petra; Milojević, Marko; Gradišnik, Lidija; Knez Hrnčič, Maša; Knez, Željko; Maver, Uroš. 2021. "Different Cannabis sativa Extraction Methods Result in Different Biological Activities against a Colon Cancer Cell Line and Healthy Colon Cells" Plants 10, no. 3: 566. https://doi.org/10.3390/plants10030566

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop