Next Article in Journal
Seasonal Variations of the Nutritive Value and Phytotherapeutic Potential of Cladium mariscus L. (Pohl.) Targeting Ruminant’s Production
Previous Article in Journal
Cell Wall Calcium and Hemicellulose Have a Role in the Fruit Firmness during Storage of Blueberry (Vaccinium spp.)
Previous Article in Special Issue
Selection and Validation of Reference Genes for Quantitative RT-PCR Analysis in Corylus heterophylla Fisch. × Corylus avellana L.
Article

Microscopic and Transcriptomic Analysis of Pollination Processes in Self-Incompatible Taraxacum koksaghyz

1
Institute of Plant Biology and Biotechnology, University of Muenster, Schlossplatz 8, 48143 Muenster, Germany
2
Fraunhofer Institute for Molecular Biology and Applied Ecology IME, Schlossplatz 8, 48143 Muenster, Germany
*
Author to whom correspondence should be addressed.
Academic Editor: Sota Fujii
Plants 2021, 10(3), 555; https://doi.org/10.3390/plants10030555
Received: 26 February 2021 / Revised: 12 March 2021 / Accepted: 13 March 2021 / Published: 16 March 2021
(This article belongs to the Special Issue Self-Incompatibility in Plants)
The transition of the Russian dandelion Taraxacum koksaghyz (Asteraceae) to a profitable, alternative crop producing natural rubber and inulin requires the optimization of several agronomic traits, cultivation conditions and harvesting procedures to improve the yield. However, efficient breeding is hindered by the obligatory sexual outcrossing of this species. Several other asters have been investigated to determine the mechanism of self-incompatibility, but the underlying molecular basis remains unclear. We therefore investigated the self-pollination and cross-pollination of two compatible T. koksaghyz varieties (TkMS2 and TkMS3) by microscopy and transcriptomic analysis to shed light on the pollination process. Self-pollination showed typical sporophytic self-incompatibility characteristics, with the rare pollen swelling at the pollen tube apex. In contrast, cross-pollination was characterized by pollen germination and penetration of the stigma by the growing pollen tubes. RNA-Seq was used to profile gene expression in the floret tissue during self-pollination and cross-pollination, and the differentially expressed genes were identified. This revealed three candidates for the early regulation of pollination in T. koksaghyz, which can be used to examine self-incompatibility mechanisms in more detail and to facilitate breeding programs. View Full-Text
Keywords: self-incompatibility; Asteraceae; Taraxacum koksaghyz; microscopy; RNA-Seq; digital gene expression self-incompatibility; Asteraceae; Taraxacum koksaghyz; microscopy; RNA-Seq; digital gene expression
Show Figures

Figure 1

MDPI and ACS Style

Wollenweber, T.E.; van Deenen, N.; Roelfs, K.-U.; Prüfer, D.; Gronover, C.S. Microscopic and Transcriptomic Analysis of Pollination Processes in Self-Incompatible Taraxacum koksaghyz. Plants 2021, 10, 555. https://doi.org/10.3390/plants10030555

AMA Style

Wollenweber TE, van Deenen N, Roelfs K-U, Prüfer D, Gronover CS. Microscopic and Transcriptomic Analysis of Pollination Processes in Self-Incompatible Taraxacum koksaghyz. Plants. 2021; 10(3):555. https://doi.org/10.3390/plants10030555

Chicago/Turabian Style

Wollenweber, Tassilo E., Nicole van Deenen, Kai-Uwe Roelfs, Dirk Prüfer, and Christian S. Gronover. 2021. "Microscopic and Transcriptomic Analysis of Pollination Processes in Self-Incompatible Taraxacum koksaghyz" Plants 10, no. 3: 555. https://doi.org/10.3390/plants10030555

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop