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Early Defense Mechanisms of Brassica oleracea in Response to Attack by Xanthomonas campestris pv. campestris

1
Molecular Genetics and Genomics Laboratory, Department of Horticulture, College of Agriculture and Life Science, Chungnam National University, Daejeon 34134, Korea
2
Moscow Timiryazev Agricultural Academy, Russian State Agrarian University, Timiryazevskaya St. 49, 127550 Moscow, Russia
3
Institute of Agricultural Science, Chungnam National University, Daejeon 34134, Korea
*
Author to whom correspondence should be addressed.
Academic Editor: Wolfgang Friedt
Plants 2021, 10(12), 2705; https://doi.org/10.3390/plants10122705
Received: 5 November 2021 / Revised: 6 December 2021 / Accepted: 6 December 2021 / Published: 9 December 2021
Black rot disease, caused by Xanthomonas campestris pv. campestris (Xcc), results in significant yield losses in Brassica oleracea crops worldwide. To find black rot disease-resistant cabbage lines, we carried out pathogenicity assays using the scissor-clipping method in 94 different B. oleracea lines. By comparing the lesion areas, we selected a relatively resistant line, Black rot Resistance 155 (BR155), and a highly susceptible line, SC31. We compared the two cabbage lines for the Xcc-induced expression pattern of 13 defense-related genes. Among them, the Xcc-induced expression level of PR1 and antioxidant-related genes (SOD, POD, APX, Trx H, and CHI) were more than two times higher in BR155 than SC31. Nitroblue tetrazolium (NBT) and diaminobenzidine tetrahydrochloride (DAB) staining analysis showed that BR155 accumulated less Xcc-induced reactive oxygen species (ROS) than did SC31. In addition, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assays showed that BR155 had higher antioxidant activity than SC31. This study, focused on the defense responses of cabbage during the early biotrophic stage of infection, indicated that Xcc-induced ROS might play a role in black rot disease development. We suggest that non-enzymatic antioxidants are important, particularly in the early defense mechanisms of cabbage against Xcc. View Full-Text
Keywords: black rot; cabbage; disease resistance; antioxidant; Xanthomonas campestris pv. campestris black rot; cabbage; disease resistance; antioxidant; Xanthomonas campestris pv. campestris
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MDPI and ACS Style

Lu, L.; Monakhos, S.G.; Lim, Y.P.; Yi, S.Y. Early Defense Mechanisms of Brassica oleracea in Response to Attack by Xanthomonas campestris pv. campestris. Plants 2021, 10, 2705. https://doi.org/10.3390/plants10122705

AMA Style

Lu L, Monakhos SG, Lim YP, Yi SY. Early Defense Mechanisms of Brassica oleracea in Response to Attack by Xanthomonas campestris pv. campestris. Plants. 2021; 10(12):2705. https://doi.org/10.3390/plants10122705

Chicago/Turabian Style

Lu, Lu, Sokrat G. Monakhos, Yong P. Lim, and So Y. Yi. 2021. "Early Defense Mechanisms of Brassica oleracea in Response to Attack by Xanthomonas campestris pv. campestris" Plants 10, no. 12: 2705. https://doi.org/10.3390/plants10122705

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