Persistence of the Effects of Se-Fertilization in Olive Trees over Time, Monitored with the Cytosolic Ca2+ and with the Germination of Pollen
Abstract
:1. Introduction
2. Results
2.1. Scanning Electron Microscopy Images of Olive Pollen Grains
2.2. Speciation of Selenium in Olive Pollen
2.3. The Cytosolic Ca2+ Tested in Pollen during Oxidative Stress
2.4. The Cytosolic Ca2+ Tested in Olive Pollen in the Presence of the Extracts of the Germinative Apexes
2.5. Germination of Olive Pollen Grains in Oxidative Stress
3. Discussion
4. Materials and Methods
4.1. Reagents
4.2. Plant Material, Growing Conditions and Pollen and Vegetative Apexes Collection
4.3. Extracts of Vegetative Apexes of Olive Trees
4.4. Determination of Total Selenium in Olive Pollens and Vegetative Apexes
4.5. Se Speciation with HPLC ICPMS
4.6. Measurement of Cytosolic Ca2+
4.7. Pollen Germination
4.8. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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MeSeCys ppb | Se Met ppb | Se (IV) ppb | Se (VI) ppb | Se Total ppb | |
---|---|---|---|---|---|
C8 | 23 ± 3 a | 858.2 ± 15 a | 300.5 ± 8 a | 1725.0 ± 32 a | 3210 ± 30 a |
C20 | 41 ± 4 a | 778.7 ± 14 a | 460.7 ± 9 b | 1883.2 ± 33 a | 3600 ± 29 a |
T8 | 153 ± 10 b | 4895.2 ± 23 c | 218.4 ± 7 a | 15,860.0 ± 34 b | 28,370 ± 51 b |
T20 | 211.8 ± 12 b | 2541.2 ± 22 b | 582.4 ± 11 b | 14,563.0 ± 31 b | 17,900 ± 41 b |
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Del Pino, A.M.; Regni, L.; D’Amato, R.; Di Michele, A.; Proietti, P.; Palmerini, C.A. Persistence of the Effects of Se-Fertilization in Olive Trees over Time, Monitored with the Cytosolic Ca2+ and with the Germination of Pollen. Plants 2021, 10, 2290. https://doi.org/10.3390/plants10112290
Del Pino AM, Regni L, D’Amato R, Di Michele A, Proietti P, Palmerini CA. Persistence of the Effects of Se-Fertilization in Olive Trees over Time, Monitored with the Cytosolic Ca2+ and with the Germination of Pollen. Plants. 2021; 10(11):2290. https://doi.org/10.3390/plants10112290
Chicago/Turabian StyleDel Pino, Alberto Marco, Luca Regni, Roberto D’Amato, Alessandro Di Michele, Primo Proietti, and Carlo Alberto Palmerini. 2021. "Persistence of the Effects of Se-Fertilization in Olive Trees over Time, Monitored with the Cytosolic Ca2+ and with the Germination of Pollen" Plants 10, no. 11: 2290. https://doi.org/10.3390/plants10112290