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Article

Comprehensive Analyses of NAC Transcription Factor Family in Almond (Prunus dulcis) and Their Differential Gene Expression during Fruit Development

Atta-ur-Rahman School of Applied Bioscience, National University of Sciences and Technology, H-12, Kashmir Highway, Islamabad 44000, Pakistan
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Academic Editor: Juan A. Salazar
Plants 2021, 10(10), 2200; https://doi.org/10.3390/plants10102200
Received: 23 August 2021 / Revised: 13 September 2021 / Accepted: 15 September 2021 / Published: 16 October 2021
(This article belongs to the Special Issue Fruit Quality and Ripening in Prunus)
As plant specific transcription factors, NAC (NAM, ATAF1/2, CUC2) domain is involved in the plant development and stress responses. Due to the vitality of NAC gene family, BLASTp was performed to identify NAC genes in almond (Prunus dulcis). Further, phylogenetic and syntenic analyses were performed to determine the homology and evolutionary relationship. Gene duplication, gene structure, motif, subcellular localization, and cis-regulatory analyses were performed to assess the function of PdNAC. Whereas RNA-seq analysis was performed to determine the differential expression of PdNAC in fruits at various developmental stages. We identified 106 NAC genes in P. dulcis genome and were renamed according to their chromosomal distribution. Phylogenetic analysis in both P. dulcis and Arabidopsis thaliana revealed the presence of 14 subfamilies. Motif and gene structure followed a pattern according to the PdNAC position in phylogenetic subfamilies. Majority of NAC are localized in the nucleus and have ABA-responsive elements in the upstream region of PdNAC. Differential gene expression analyses revealed one and six PdNAC that were up and down-regulated, respectively, at all development stages. This study provides insights into the structure and function of PdNAC along with their role in the fruit development to enhance an understanding of NAC in P. dulcis. View Full-Text
Keywords: genome wide identification; NAC; transcription factor; phylogenetic analysis; syntenic analysis; gene duplication; RNA-seq; differential gene expression genome wide identification; NAC; transcription factor; phylogenetic analysis; syntenic analysis; gene duplication; RNA-seq; differential gene expression
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MDPI and ACS Style

Zafar, Z.; Fatima, S.; Bhatti, M.F. Comprehensive Analyses of NAC Transcription Factor Family in Almond (Prunus dulcis) and Their Differential Gene Expression during Fruit Development. Plants 2021, 10, 2200. https://doi.org/10.3390/plants10102200

AMA Style

Zafar Z, Fatima S, Bhatti MF. Comprehensive Analyses of NAC Transcription Factor Family in Almond (Prunus dulcis) and Their Differential Gene Expression during Fruit Development. Plants. 2021; 10(10):2200. https://doi.org/10.3390/plants10102200

Chicago/Turabian Style

Zafar, Zeeshan, Sidra Fatima, and Muhammad F. Bhatti 2021. "Comprehensive Analyses of NAC Transcription Factor Family in Almond (Prunus dulcis) and Their Differential Gene Expression during Fruit Development" Plants 10, no. 10: 2200. https://doi.org/10.3390/plants10102200

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