Screening for Selective Anticancer Activity of 65 Extracts of Plants Collected in Western Andalusia, Spain
Abstract
:1. Introduction
2. Results and Discussion
3. Materials and Methods
3.1. Plant Material
3.2. Preparation of the Extracts
3.3. Chemicals and Cell Lines
3.4. Cell Viability Assays
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Extract | Plant Name (Family) | Part Used | Voucher Number (SEV) | Origin | IC50 (MTT) (Mean ± SEM, µg/mL) | S.I. (Mean ± SEM) | |
---|---|---|---|---|---|---|---|
A549 (Cancer) | MRC-5 (Normal) | ||||||
1 | Acis autumnalis (L.) Sweet (Amaryllidaceae) | Whole plant | 284654 | Seville | 32.9 ± 9.6 | 59.1 ± 18.0 | 1.9 ± 0.6 |
2 | Anagallis monelli L. (Primulaceae) | Aerial Parts | 284675 | Cádiz | 3.2 ± 1.4 | 0.6 ± 0.2 | 0.3 ± 0.2 |
3 | Anagallis monelli L. (Primulaceae) | Root | 284675 | Cádiz | 7.4 ± 5.2 | 0.9 ± 0.5 | 0.3 ± 0.3 |
4 | Anthyllis hamosa Desf. (Leguminosae) | Whole plant | 284685 | Huelva | 260.9 ± 14.7 | 317.7 ± 3.1 | 1.2 ± 0.1 |
5 | Aristolochia baetica L. (Aristolochiaceae) | Fruits | 284674 | Seville | 249.1 ± 2.5 | 649.9 ± 345.0 | 2.6 ± 1.4 |
6 | Aristolochia baetica L. (Aristolochiaceae) | Leaves | 284674 | Seville | 91.8 ± 70.5 | 66.0 ± 39.9 | 0.9 ± 0.3 |
7 | Armeria pungens (Link) Hoffmanns. & Link (Plumbaginaceae) | Flowering aerial parts | 284687 | Huelva | 143.4 ± 64.3 | 550.7 ± 352.2 | 3.6 ± 1.1 |
8 | Armeria velutina Welw. ex Boiss. & Reut. (Plumbaginaceae) | Whole plant | 284689 | Huelva | 25.7 ± 1.9 | 36.7 ± 0.9 | 1.4 ± 0.1 |
9 | Campanula lusitanica L. (Campanulaceae) | Whole plant | 284667 | Seville | 223.4 ± 51.2 | 263.7 ± 34.2 | 1.3 ± 0.4 |
10 | Cascabela thevetia (L.) Lippold (Apocynaceae) | Leaves | 284662 | Seville | 0.14 ± 0.02 | 1.6 ± 0.5 | 26.6 ± 11.6 |
11 | Centaurea sphaerocephala L. (Compositae) | Whole plant | 284683 | Huelva | 134.2 ± 82.4 | 160.4 ± 33.8 | 1.7 ± 0.8 |
12 | Centaurea sphaerocephala L. (Compositae) | Flowers | 284676 | Cádiz | 139.1 ± 40.1 | 108.7 ± 7.7 | 1.7 ± 1.1 |
13 | Centaurea sphaerocephala L. (Compositae) | Leaves | 284676 | Cádiz | 43.1 ± 11.1 | 114.7 ± 14.4 | 3.0 ± 0.8 |
14 | Cistus crispus L. (Cistaceae) | Leaves | 284660 | Seville | 52.4 ± 5.1 | 126.4 ± 34.3 | 2.5 ± 0.7 |
15 | Cistus crispus L. (Cistaceae) | Root | 284660 | Seville | 24.9 ± 0.7 | 58.8 ± 14.8 | 2.1 ± 0.7 |
16 | Cistus salviifolius L. (Cistaceae) | Leaves | 284653 | Seville | 56.6 ± 12.6 | 142.7 ± 20.4 | 3.0 ± 0.9 |
17 | Cleome violacea L. (Cleomaceae) | Aerial Parts | 284668 | Seville | 253.9 ± 39.9 | 261.1 ± 3.4 | 1.1 ± 0.2 |
18 | Digitalis purpurea L. (Plantaginaceae) | Leaves | 284691 | Huelva | 0.17 ± 0.15 | 1.06 ± 0.69 | 9.3 ± 0.5 |
19 | Dorycnium rectum (L.) Ser. (Leguminosae) | Flowers | 284690 | Seville | 102.1 ± 40.2 | 182.9 ± 46.1 | 4.3 ± 3.2 |
20 | Dorycnium rectum (L.) Ser. (Leguminosae) | Leaves | 284690 | Seville | 293.6 ± 21.2 | 322.1 ± 27.4 | 1.1 ± 0.2 |
21 | Echium gaditanum Boiss. (Boraginaceae) | Aerial Parts | 284684 | Huelva | 196.1 ± 107.2 | 325.6 ± 44.0 | 1.1 ± 0.1 |
22 | Elaeoselinum foetidum (L.) Boiss. (Apiaceae) | Flowers | 284670 | Seville | 119.0 ± 16.2 | 262.1 ± 8.7 | 2.3 ± 0.3 |
23 | Elaeoselinum foetidum (L.) Boiss. (Apiaceae) | Leaves | 284670 | Seville | 246.5 ± 25.5 | 295.0 ± 36.4 | 1.2 ± 0.0 |
24 | Erica arborea L. (Ericaceae) | Bark | 284657 | Seville | 32.2 ± 5.6 | 62.1 ± 28.9 | 1.8 ± 0.7 |
25 | Erica arborea L. (Ericaceae) | Leaves | 284657 | Seville | 45.7 ± 7.4 | 158.4 ± 43.6 | 3.9 ± 1.4 |
26 | Erophaca baetica (L.) Boiss. (Leguminosae) | Leaves | 284673 | Seville | >1000 | >1000 | N.D. |
27 | Frangula alnus Mill. (Rhamnaceae) | Bark | 284680 | Huelva | 32.8 ± 7.4 | 339.3 ± 74.1 | 12.4 ± 3.3 |
28 | Frangula alnus Mill. (Rhamnaceae) | Leaves | 284680 | Huelva | 28.6 ± 3.5 | 74.0 ± 38.5 | 2.4 ± 1.0 |
29 | Genista hirsuta M.Vahl (Leguminosae) | Aereal Parts | 284671 | Seville | 273.6 ± 5.8 | 363.9 ± 27.9 | 1.3 ± 0.1 |
30 | Halimium calycinum (L.) K.Koch (Cistaceae) | Leaves | 284656 | Seville | 47.4 ± 10.0 | 135.4 ± 23.6 | 4.3 ± 1.9 |
31 | Halimium calycinum (L.) K.Koch (Cistaceae) | Root | 284656 | Seville | 60.1 ± 17.7 | 101.1 ± 13.6 | 2.2 ± 0.8 |
32 | Halimium halimifolium (L.) Willk. (Cistaceae) | Leaves | 284659 | Seville | 47.9 ± 10.9 | 105.9 ± 21.4 | 2.7 ± 0.9 |
33 | Halimium halimifolium (L.) Willk. (Cistaceae) | Root | 284659 | Seville | 67.5 ± 16.0 | 110.2 ± 33.4 | 1.8 ± 0.8 |
34 | Hedysarum coronarium L. (Leguminosae) | Flowers | 284677 | Cádiz | 230.5 ± 54.6 | 306.9 ± 55.1 | 1.4 ± 0.1 |
35 | Hedysarum coronarium L. (Leguminosae) | Fruits | 284677 | Cádiz | 247.0 ± 3.2 | 294.5 ± 0.4 | 1.2 ± 0.0 |
36 | Hedysarum coronarium L. (Leguminosae) | Leaves | 284677 | Cádiz | 173.9 ± 60.6 | 262.0 ± 32.3 | 2.1 ± 0.9 |
37 | Iberis ciliata subsp. contracta (Pers.) Moreno (Brassicaceae) | Whole plant | 284688 | Huelva | 0.31 ± 0.06 | 2.31 ± 0.88 | 13.0 ± 4.7 |
38 | Jasione montana L. (Campanulaceae) | Whole plant | 284666 | Seville | 145.0 ± 26.0 | 301.6 ± 69.2 | 2.0 ± 0.1 |
39 | Juniperus macrocarpa Sm. (Cupressaceae) | Monosperma cones | 284682 | Huelva | 146.1 ± 125.9 | >1000 | >20 |
40 | Juniperus macrocarpa Sm. (Cupressaceae) | Aerial Parts | 284682 | Huelva | 3.7 ± 1.9 | 2.8 ± 1.0 | 0.8 ± 0.1 |
41 | Malcolmia lacera (L.) DC. (Brassicaceae) | Whole plant | 284664 | Seville | 322.3 ± 86.7 | 295.0 ± 5.9 | 1.0 ± 0.3 |
42 | Malva hispanica L. (Malvaceae) | Aerial Parts | 284669 | Seville | >1000 | 703.1 ± 51.1 | N.D. |
43 | Ononis subspicata Lag. (Leguminosae) | Whole plant | 284695 | Huelva | 63.9 ± 23.6 | 232.3 ± 29.5 | 4.6 ± 1.4 |
44 | Ornithopus compressus L. (Leguminosae) | Whole plant | 284693 | Seville | 340.7 ± 28.4 | 583.8 ± 150.0 | 1.8 ± 0.6 |
45 | Ornithopus sativus Brot. (Leguminosae) | Whole plant | 284692 | Seville | 326.6 ± 37.5 | 699.0 ± 262.9 | 2.3 ± 1.1 |
46 | Pancratium maritimum L. (Amaryllidaceae) | Bulb | 284681 | Huelva | 3.4 ± 0.2 | 74.1 ± 56.1 | 19.7 ± 14.6 |
47 | Pycnocomon rutifolium (Vahl) Hoffmanns. & Link (Caprifoliaceae) | Leaves | 284678 | Cádiz | 71.4 ± 40.4 | 70.6 ± 18.1 | 2.2 ± 1.4 |
48 | Pycnocomon rutifolium (Vahl) Hoffmanns. & Link (Caprifoliaceae) | Root | 284678 | Cádiz | 262.5 ± 131,9 | 242.9 ± 56.7 | 1.1 ± 0.3 |
49 | Ranunculus peltatus Schrank (Ranunculaceae) | Whole plant | 284672 | Seville | 186.7 ± 54.6 | 170.1 ± 58.8 | 0.9 ± 0.1 |
50 | Rhamnus lycioides subsp. oleoides (L.) Jahand. & Maire (Rhamnaceae) | Bark | 284655 | Seville | 332.2 ± 61.9 | 599.4 ± 122.1 | 2.1 ± 0.8 |
51 | Rhamnus lycioides subsp. oleoides (L.) Jahand. & Maire (Rhamnaceae) | Leaves | 284655 | Seville | 151.5 ± 16.7 | 421.3 ± 176.5 | 3.1 ± 1.6 |
52 | Rhamnus lycioides subsp. oleoides (L.) Jahand. & Maire (Rhamnaceae) | Root | 284655 | Seville | 257.0 ± 76.6 | 442.3 ± 35.1 | 2.3 ± 0.8 |
53 | Scrophularia frutescens L. (Scrophulariaceae) | Whole plant | 284686 | Huelva | 281.5 ± 9.2 | 297.6 ± 8.6 | 1.1 ± 0.1 |
54 | Stauracanthus genistoides (Brot.) G. Sampaio (Leguminosae) | Aerial Parts | 284679 | Huelva | 278.4 ± 32.9 | 546.5 ± 53.5 | 2.0 ± 0.4 |
55 | Tamarix canariensis Willd. (Tamaricaceae) | Flowers | 284650 | Seville | 212.8 ± 10.2 | 336.3 ± 3.6 | 1.6 ± 0.1 |
56 | Tamarix canariensis Willd. (Tamaricaceae) | Leaves | 284650 | Seville | 92.7 ± 24.6 | 192.1 ± 33.0 | 2.2 ± 0.3 |
57 | Taxus baccata L. (Taxaceae) | Leaves | 284621 | Seville | 0.86 ± 0.27 | 146.9 ± 87.8 | 157.3 ± 110.6 |
58 | Tetraclinis articulata (Vahl) Mast. (Cupressaceae) | Leaves | 284663 | Seville | 0.37 ± 0.03 | 129.5 ± 64.0 | 378.3 ± 178.1 |
59 | Teucrium fruticans L. (Lamiaceae) | Leaves | 284658 | Seville | 157.1 ± 30.5 | 433.0 ± 112.3 | 2.8 ± 0.7 |
60 | Thymus mastichina (L.) L. (Lamiaceae) | Whole plant | 284694 | Huelva | 36.8 ± 7.7 | 277.7 ± 40.6 | 8.2 ± 2.0 |
61 | Tolpis barbata (L.) Gaertn. (Compositae) | Whole plant | 284665 | Seville | 86.5 ± 28.3 | 43.7 ± 12.2 | 0.5 ± 0.0 |
62 | Ulex parviflorus Pourr. subsp. parviflorus (Leguminosae) | Flowers | 284652 | Seville | 361.7 ± 89.6 | 876.1 ± 426.1 | 4.0 ± 2.9 |
63 | Ulex parviflorus Pourr. subsp. parviflorus (Leguminosae) | Leaves | 284652 | Seville | 99.6 ± 34.8 | 270.4 ± 85.3 | 3.4 ± 1.2 |
64 | Viburnum tinus L. (Adoxaceae) | Fruits | 284651 | Seville | 26.6 ± 6.5 | 65.4 ± 8.6 | 2.6 ± 0.3 |
65 | Viburnum tinus L. (Adoxaceae) | Leaves | 284651 | Seville | 234.8 ± 58.7 | 568.2 ± 73.0 | 2.6 ± 0.3 |
Cisplatin (Standard anticancer drug) | 10.5 ± 5.5 (µM) | 25.4 ± 7.4 (µM) | 4.2 ± 2.2 | ||||
5-Fluorouracil (Standard anticancer drug) | 101.8 ± 7.7 (µM) | >1000 (µM) | >9.9 |
IC50 (Resazurin) (Mean ± SEM, µg/mL) | IC50 (Resazurin) (Mean ± SEM, µM) | |||||||
---|---|---|---|---|---|---|---|---|
Cell Line | 10 | 18 | 37 | 46 | 57 | 58 | Cisplatin | 5-FU |
HepG2 | 24.6 ± 4.1 | 13.2 ± 3.0 | 19.4 ± 10.4 | 7.0 ± 1.3 | 632.6 ± 106.1 | 18.4 ± 17.1 | 3.5 ± 0.8 | 0.3 ± 0.2 |
SW480 | 20.7 ± 5.6 | 18.3 ± 1.2 | 1.2 ± 0.3 | 2.1 ± 0.4 | 2.2 ± 0.7 | 2.1 ± 0.8 | 2.0 ± 0.5 | 3.4 ± 1.0 |
U2OS | 9.2 ± 1.5 | 8.8 ± 0.8 | 1.9 ± 0.2 | 2.8 ± 0.7 | 4.0 ± 1.2 | 2.1 ± 0.9 | 4.0 ± 1.0 | 7.2 ± 1.0 |
HeLa | 4.7 ± 0.1 | 4.9 ± 0.2 | 3.5 ± 0.5 | 5.4 ± 0.4 | 2.5 ± 0.4 | 1.3 ± 0.6 | 4.8 ± 0.1 | 66.5 ± 27.0 |
PC3 | 4.7 ± 1.5 | 2.6 ± 0.4 | 5.6 ± 2.1 | 5.4 ± 1.2 | 123.8 ± 42.7 | 0.50 ± 0.04 | 7.3 ± 4.6 | 1.5 ± 0.8 |
MCF7 | 10.0 ± 0.5 | 6.5 ± 1.0 | 4.0 ± 0.9 | 5.9 ± 0.8 | 37.1 ± 4.9 | 0.7 ± 0.1 | 8.0 ± 3.0 | 1.1 ± 0.8 |
NB4 | 2.9 ± 0.7 | 1.8 ± 0.5 | 0.5 ± 0.2 | 1.2 ± 0.2 | 2.8 ± 0.2 | 0.2 ± 0.1 | 0.08 ± 0.04 | 2.7 ± 0.3 |
HL-60 | 3.5 ± 0.4 | 3.6 ± 0.3 | 0.8 ± 0.2 | 1.0 ± 0.3 | 3.3 ± 0.2 | 0.7 ± 0.3 | 0.2 ± 0.1 | 0.6 ± 0.5 |
BJ-hTERT | 3.7 ± 0.4 | 2.8 ± 0.3 | 3.4 ± 0.5 | 3.4 ± 0.1 | 4.0 ± 0.6 | 0.8 ± 0.3 | 1.2 ± 0.4 | 2.6 ± 0.6 |
BJ-SV40T | 2.9 ± 0.4 | 2.1 ± 0.4 | 2.9 ± 0.4 | 3.2 ± 0.4 | 2.8 ± 0.2 | 1.0 ± 0.4 | 0.7 ± 0.2 | 5.8 ± 0.7 |
BJ-RASV12 | 2.9 ± 0.4 | 1.8 ± 0.7 | 4.0 ± 1.4 | 2.0 ± 0.7 | 3.3 ± 0.2 | 0.6 ± 0.2 | 1.1 ± 0.1 | 1.7 ± 0.8 |
Cell Line | IC50 (Resazurin) (Media ± SEM, µg/mL) |
---|---|
58 | |
A64-CLS | 4.7 ± 0.3 |
AN3Ca | 4.5 ± 0.9 |
Calu-1 | 4.7 ± 0.2 |
GAMG | 4.5 ± 0.6 |
HNO97 | 4.5 ± 0.3 |
HT29 | 4.4 ± 0.4 |
KATO III | 4.4 ± 0.7 |
MDA-MB-231 | 4.7 ± 0.2 |
MeWo | 4.3 ± 0.2 |
PC-3 | 4.5 ± 1.0 |
Sk-Br-3 | 4.9 ± 0.0 |
Sk-OV-3 | 4.7 ± 0.1 |
T24 | 4.5 ± 0.3 |
UACC-62 | 4.5 ± 0.2 |
Extract | Plant Name | Collection Coordinates |
---|---|---|
1 | Acis autumnalis (L.) Sweet | 37°14′22.06″ N 6°11′37.85″ W |
2–3 | Anagallis monelli L. | 36°36′15.24″ N 6°16′2.76″ W |
4 | Anthyllis hamosa Desf. | 37°04′25.2″ N 6°41′19.68″ W |
5–6 | Aristolochia baetica L. | 37°14′16.68″ N 6°11′48.38″ W |
7 | Armeria pungens (Link) Hoffmanns. & Link | 37°04′13.73″ N 6°41′16.97″ W |
8 | Armeria velutina Welw. ex Boiss. & Reut. | 37°02′33.33″ N 6°35′53.85″ W |
9 | Campanula lusitanica L. | 37°14′14.46″ N 6°11′55.8″ W |
10 | Cascabela thevetia (L.) Lippold | 37°22′59.8″ N 5°59′27.36″ W |
11–13 | Centaurea sphaerocephala L. | 37°05′40.18″ N 6°43′37.9″ W |
14–15 | Cistus crispus L. | 37°14′22.06″ N 6°11′37.85″ W |
16 | Cistus salvifolius L. | 37°14′19.74″ N 6°11′40.71″ W |
17 | Cleome violacea L. | 37°14′23.37″ N 6°11′52.72″ W |
18 | Digitalis purpurea L. | 37°27′30.78″ N 6°41′20.3″ W |
19–20 | Dorycnium rectum (L.) Ser. | 37°20′14.71″ N 5°51′27.13″ W |
21 | Echium gaditanum Boiss. | 37°04′11.69″ N 6°41′17.16″ W |
22–23 | Elaeoselinum foetidum (L.) Boiss. | 37°14′15.72″ N 6°11′50.86″ W |
24–25 | Erica arborea L. | 37°14′38.35″ N 6°11′49.92″ W |
26 | Erophaca baetica (L.) Boiss. | 37°14′17.72″ N 6°11′46.41″ W |
27–28 | Frangula alnus Mill. | 37°05′40.46″ N 6°43′34.47″ W |
29 | Genista hirsuta M. Vahl | 37°14′16.70 “N 6°11′49.05″ W |
30–31 | Halimium calycinum (L.) K. Koch | 37°14′21.27N 6°11′38.12″ W |
32–33 | Halimium halimifolium (L.) Willk. | 37°14′23.43″ N 6°11′38.77″ W |
34–36 | Hedysarum coronarium L. | 36°36′39.92″ N 6°16′46.6″ W |
37 | Iberis ciliata subsp. contracta (Pers.) Moreno | 37°04′47.25″ N 6°41’13.82″ W |
38 | Jasione montana L. | 37°13′45.76″ N 6°9′16.08″ W |
39–40 | Juniperus macrocarpa Sm. | 37°04′13.53″ N 6°41′16.34″ W |
41 | Malcolmia lacera (L.) DC. | 37°13′45.76″ N 6°9′16.08″ W |
42 | Malva hispanica L. | 37°14′16.09″ N 6°11′51.89″ W |
43 | Ononis subspicata Lag. | 37°04′45.90″ N 6°41′14.14″ W |
44 | Ornithopus compressus L. | 37°13′45.76″ N 6°9′16.08″ W |
45 | Ornithopus sativus Brot. | 37°13′45.76″ N 6°9′16.08″ W |
46 | Pancratium maritimum L. | 37°04′11.14″ N 6°41′16.29″ W |
47–48 | Pycnocomon rutifolium (Vahl) Hoffmanns. & Link | 36°36′12.69″ N 6°15′54.45″ W |
49 | Ranunculus peltatus Schrank | 37°13′45.76″ N 6°9′16.08″ W |
50–52 | Rhamnus lycioides subsp. oleoides (L.) Jahand. & Maire | 37°14′25.18″ N 6°11′38.35″ W |
53 | Scrophularia frutescens L. | 37°04′47.25″ N 6°41′13.82″ W |
54 | Stauracanthus genistoides (Brot.) G. Sampaio | 37°04′44.55″ N 6°41′16.05″ W |
55–56 | Tamarix canariensis Willd. | 37°15′45.64″ N 5°59′50.9″ W |
57 | Taxus baccata L. | 37°22′27″ N 5°59′19″ W |
58 | Tetraclinis articulata (Vahl) Mast. | 37°22′22.18″ N 5°59′10.75″ W |
59 | Teucrium fruticans L. | 37°14′42.60″ N 6°11′52.78″ W |
60 | Thymus mastichina (L.) L. | 37°02′33.33″ N 6°35′53.85″ W |
61 | Tolpis barbata (L.) Gaertn. | 37°13′45.76″ N 6°9′16.08″ W |
62–63 | Ulex parviflorus Pourr. subsp. parviflorus | 37°13′48.06″ N 6°1′31.4″ W |
64–65 | Viburnum tinus L. | 37°22′27″ N 5°59′19″ W |
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Calderón-Montaño, J.M.; Martínez-Sánchez, S.M.; Jiménez-González, V.; Burgos-Morón, E.; Guillén-Mancina, E.; Jiménez-Alonso, J.J.; Díaz-Ortega, P.; García, F.; Aparicio, A.; López-Lázaro, M. Screening for Selective Anticancer Activity of 65 Extracts of Plants Collected in Western Andalusia, Spain. Plants 2021, 10, 2193. https://doi.org/10.3390/plants10102193
Calderón-Montaño JM, Martínez-Sánchez SM, Jiménez-González V, Burgos-Morón E, Guillén-Mancina E, Jiménez-Alonso JJ, Díaz-Ortega P, García F, Aparicio A, López-Lázaro M. Screening for Selective Anticancer Activity of 65 Extracts of Plants Collected in Western Andalusia, Spain. Plants. 2021; 10(10):2193. https://doi.org/10.3390/plants10102193
Chicago/Turabian StyleCalderón-Montaño, José Manuel, Sara María Martínez-Sánchez, Víctor Jiménez-González, Estefanía Burgos-Morón, Emilio Guillén-Mancina, Julio José Jiménez-Alonso, Patricia Díaz-Ortega, Felipe García, Abelardo Aparicio, and Miguel López-Lázaro. 2021. "Screening for Selective Anticancer Activity of 65 Extracts of Plants Collected in Western Andalusia, Spain" Plants 10, no. 10: 2193. https://doi.org/10.3390/plants10102193