The Efficient and Easy Micropropagation Protocol of Phyllanthus niruri
Abstract
:1. Introduction
2. Results
2.1. Surface Sterilization
2.2. Shoot Multiplication Using Different Basal Medium and Its Strength
2.3. Shoot Multiplication Using Different Plant Growth Regulators and Its Concentrations
2.4. In Vitro Rooting and Acclimatization
3. Discussion
4. Materials and Methods
4.1. Plant Materials and Maintenance
4.2. Surface Sterilization of Explants
4.3. Basal Media and Its Strength
4.4. Plant Growth Regulators and Its Concentrations
4.5. In Vitro Rooting and Acclimatization
4.6. Experimental Design and Data Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Treatment/Chemical Sterilants | Concentration | Percentage of Contamination (%) |
---|---|---|
Clorox® (%) | 10 | 10.22 ± 2.28 ab |
20 | 6.67 ± 0.7 bc | |
30 | 4.44 ± 1.21 c | |
Nano Silver (ppm) | 10 | 14.22 ± 1.13 a |
20 | 12.44 ± 1.13 a | |
30 | 12.00 ± 2.28 a |
Treatment | Number of Shoots | Length of Shoot (cm) | Number of Leaves |
---|---|---|---|
MSH | 2.00 ± 0.57 bc | 2.79 ± 0.14 bc | 23.11 ± 0.94 a |
MSF | 2.33 ± 0.33 ab | 3.11 ± 0.43 b | 27.91 ± 0.90 a |
MSD | 0.67 ± 0.33 c | 1.5 ± 0.23 c | 4.88 ± 0.89 b |
B5H | 3.67 ± 0.33 a | 3.52 ± 0.12 ab | 24.25 ± 0.47 a |
B5F | 3.00 ± 0.57 ab | 4.84 ± 0.33 a | 35.94 ± 1.81 a |
B5D | 1.67 ± 0.66 bc | 3.58 ± 0.69 ab | 22.83 ± 0.92 a |
Cytokinin (µM) | Number of Shoots | Length of Shoot (cm) | Number of Leaves |
---|---|---|---|
Control | 5.0 ± 0.50 ab | 3.68 ± 0.60 ab | 27.33 ± 1.15 a |
BAP 2.5 | 4.45 ± 0.96 abc | 3.09 ± 0.45 bcd | 20.89 ± 1.29 abcd |
BAP 5.0 | 5.33 ± 0.57 a | 2.5 ± 0.51 bcde | 24.11 ± 2.00 abc |
BAP 7.5 | 4.45 ± 0.77 abc | 2.47 ± 0.44 bcde | 23.22 ± 1.83 abc |
BAP 10.0 | 2.55 ± 0.61 e | 1.67 ± 0.15 e | 16.00 ± 1.83 bcd |
Kn 2.5 | 5.33 ± 0.77 a | 3.38 ± 0.42 abc | 25.33 ± 0.62 ab |
Kn 5.0 | 4.34 ± 0.33 abcd | 4.31 ± 0.89 a | 22.45 ± 0.49 abc |
Kn 7.5 | 4.1 1± 0.29 abcd | 2.38 ± 0.30 cde | 18.67 ± 2.05 abcd |
Kn 10.0 | 3.67 ± 0.50 bcde | 2.73 ± 0.58 bcde | 18.33 ± 1.50 abcd |
Zn 2.5 | 3.55 ± 0.39 bcde | 2.52 ± 0.07 bcde | 15.46 ± 1.61 cd |
Zn 5.0 | 2.89 ± 0.11 de | 2.01 ± 0.09 de | 17.00 ± 2.71 bcd |
Zn 7.5 | 3.22 ± 0.29 cde | 1.99 ± 0.06 de | 14.67 ± 1.64 cd |
Zn 10.0 | 4.22 ± 0.11 abcd | 2.56 ± 0.09 bcde | 2.22 ± 1.44 abc |
2iP 2.5 | 3.89 ± 0.72 abcde | 2.71± 0.36 bcde | 15.22 ± 1.36 cd |
2iP 5.0 | 3.0 ± 0.33 cde | 1.63 ± 0.20 e | 12.33 ± 0.38 d |
2iP 7.5 | 3.45 ± 0.22 cde | 2.43 ± 0.56 cde | 16.00 ± 2.54 bcd |
2iP 10.0 | 4.00 ± 0.57 abcde | 2.47 ± 0.16 bcde | 15.89 ± 1.89 bcd |
Auxin (µM) | Number of Roots | Length of Root (cm) |
---|---|---|
Control | 1.0 ± 0.40 h | 0.13 ± 0.03 f |
IBA (1.25) | 9.4 ± 1.11 cd | 1.07 ± 0.03 abc |
IBA (2.5) | 17.92 ± 1.38 a | 1.29 ± 0.11 a |
IBA (5) | 10.53 ± 0.67 bc | 0.79 ± 0.19 cde |
IAA (1.25) | 7.27 ± 0.40 de | 0.97 ± 0.09 bcd |
IAA (2.5) | 3.47 ± 0.24 g | 0.85 ± 0.05 cde |
IAA (5) | 4.87 ± 0.33 fg | 0.86 ± 0.06 cde |
NAA (1.25) | 6.93 ± 0.94 ef | 0.65 ± 0.11 e |
NAA (2.5) | 12.47 ± 0.88 b | 1.22 ± 0.08 ab |
NAA (5) | 11.87 ± 0.43 b | 0.77 ± 0.06 de |
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Suraya, A.A.; Misran, A.; Hakiman, M. The Efficient and Easy Micropropagation Protocol of Phyllanthus niruri. Plants 2021, 10, 2141. https://doi.org/10.3390/plants10102141
Suraya AA, Misran A, Hakiman M. The Efficient and Easy Micropropagation Protocol of Phyllanthus niruri. Plants. 2021; 10(10):2141. https://doi.org/10.3390/plants10102141
Chicago/Turabian StyleSuraya, Azal Anis, Azizah Misran, and Mansor Hakiman. 2021. "The Efficient and Easy Micropropagation Protocol of Phyllanthus niruri" Plants 10, no. 10: 2141. https://doi.org/10.3390/plants10102141