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Article

Ribosome Incorporation Transdifferentiates Chick Primary Cells and Induces Their Proliferation by Secreting Growth Factors

by
Shota Inoue
1,
Arif Istiaq
1,†,
Anamika Datta
1,‡,
Mengxue Lu
2,
Shintaro Nakayama
1,
Kousei Takashi
1,
Nobushige Nakajo
3,
Shigehiko Tamura
4,
Ikko Kawashima
2 and
Kunimasa Ohta
1,5,*
1
Department of Stem Cell Biology, Graduate School of Systems Life Sciences, Kyushu University, 744 Motooka, Nishi-Ku, Fukuoka 819-0395, Japan
2
IntegriCulture, 8-1 Kawada-Cho, Shinjuku-ku, Tokyo 162-0054, Japan
3
Department of Biology, Faculty of Science, Kyushu University, 744 Motooka, Nishi-Ku, Fukuoka 819-0395, Japan
4
Department of Molecular Cell Biology, Faculty of Arts and Science, Kyushu University, 744 Motooka, Nishi-Ku, Fukuoka 819-0395, Japan
5
Department of Stem Cell Biology, Faculty of Arts and Science, Kyushu University, 744 Motooka, Nishi-Ku, Fukuoka 819-0395, Japan
*
Author to whom correspondence should be addressed.
†
Current address: Department of Pediatrics, Division of Genetics and Genomics Medicine, Washington University School of Medicine, St. Louis, MO 63110, USA.
‡
Current address: Department of Systems Biomedicine, Institute of Science Tokyo, Bunkyo-ku, Tokyo 113-8519, Japan.
J. Dev. Biol. 2025, 13(2), 19; https://doi.org/10.3390/jdb13020019
Submission received: 10 February 2025 / Revised: 7 April 2025 / Accepted: 20 May 2025 / Published: 1 June 2025
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Abstract

Previously, we reported that mammalian cells, specifically human dermal fibroblasts (HDFs), could be transdifferentiated by lactic acid bacteria (LAB). Later, we observed that HDFs incorporated LAB-derived ribosomes, forming the ribosome-induced cell clusters (RICs) and transdifferentiating into cells derived from all three germ layers. Based on this insight, we hypothesized that incorporating ribosomes into non-mammalian cells could reveal the universality of this mechanism and open the door to commercial applications. Our current study demonstrates that ribosome incorporation can transdifferentiate chick primary muscle-derived cells (CMCs) into adipocytes, osteoblasts, and chondrocytes. Furthermore, the culture medium supernatant from ribosome-incorporated CMCs was found to significantly enhance CMC’s proliferation. RNA-seq analysis revealed that RICs-CMC exhibit increased expression of genes related to multi-lineage cell growth. In addition, we developed a novel technological shift in meat production—the “CulNet System”—which replicates organ interactions within mechanical systems for cell-cultured meat production. While significant efforts are still required to implement this technology in a cost-effective manner, we believe that combining the “CulNet System” with ribosome-incorporated multipotent cells that have prolonged culture capability could substantially improve the scalability and cost-effectiveness of cultured chicken meat production. This report highlights a promising approach for cell-culture-based meat production, offering a sustainable alternative to traditional methods.
Keywords: chick primary cells; ribosomes; cell proliferation; cultured meat chick primary cells; ribosomes; cell proliferation; cultured meat

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MDPI and ACS Style

Inoue, S.; Istiaq, A.; Datta, A.; Lu, M.; Nakayama, S.; Takashi, K.; Nakajo, N.; Tamura, S.; Kawashima, I.; Ohta, K. Ribosome Incorporation Transdifferentiates Chick Primary Cells and Induces Their Proliferation by Secreting Growth Factors. J. Dev. Biol. 2025, 13, 19. https://doi.org/10.3390/jdb13020019

AMA Style

Inoue S, Istiaq A, Datta A, Lu M, Nakayama S, Takashi K, Nakajo N, Tamura S, Kawashima I, Ohta K. Ribosome Incorporation Transdifferentiates Chick Primary Cells and Induces Their Proliferation by Secreting Growth Factors. Journal of Developmental Biology. 2025; 13(2):19. https://doi.org/10.3390/jdb13020019

Chicago/Turabian Style

Inoue, Shota, Arif Istiaq, Anamika Datta, Mengxue Lu, Shintaro Nakayama, Kousei Takashi, Nobushige Nakajo, Shigehiko Tamura, Ikko Kawashima, and Kunimasa Ohta. 2025. "Ribosome Incorporation Transdifferentiates Chick Primary Cells and Induces Their Proliferation by Secreting Growth Factors" Journal of Developmental Biology 13, no. 2: 19. https://doi.org/10.3390/jdb13020019

APA Style

Inoue, S., Istiaq, A., Datta, A., Lu, M., Nakayama, S., Takashi, K., Nakajo, N., Tamura, S., Kawashima, I., & Ohta, K. (2025). Ribosome Incorporation Transdifferentiates Chick Primary Cells and Induces Their Proliferation by Secreting Growth Factors. Journal of Developmental Biology, 13(2), 19. https://doi.org/10.3390/jdb13020019

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