Small, Smaller, Smallest: Minimal Structural Requirements for a Fully Functional Box C/D Modification Guide RNA
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animals and Oocytes
2.2. In Vitro Transcription
2.3. In Vitro Modification Assays
2.4. 2’-O-methylation Mapping
2.5. Expression of Drosophila snoRNAs in HeLa Cells
2.6. Northern Blot
3. Results and Discussion
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Deryusheva, S.; Gall, J.G. Small, Smaller, Smallest: Minimal Structural Requirements for a Fully Functional Box C/D Modification Guide RNA. Biomolecules 2019, 9, 457. https://doi.org/10.3390/biom9090457
Deryusheva S, Gall JG. Small, Smaller, Smallest: Minimal Structural Requirements for a Fully Functional Box C/D Modification Guide RNA. Biomolecules. 2019; 9(9):457. https://doi.org/10.3390/biom9090457
Chicago/Turabian StyleDeryusheva, Svetlana, and Joseph G. Gall. 2019. "Small, Smaller, Smallest: Minimal Structural Requirements for a Fully Functional Box C/D Modification Guide RNA" Biomolecules 9, no. 9: 457. https://doi.org/10.3390/biom9090457