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Highly Glycolytic Immortalized Human Dermal Microvascular Endothelial Cells are Able to Grow in Glucose-Starved Conditions

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Departamento de Biología Molecular y Bioquímica, Facultad de Ciencias, Andalucía Tech, Universidad de Málaga, E-29071 Málaga, Spain
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IBIMA (Biomedical Research Institute of Málaga), E-29071 Málaga, Spain
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CIBER de Enfermedades Raras (CIBERER), E-29071 Málaga, Spain
*
Author to whom correspondence should be addressed.
Biomolecules 2019, 9(8), 332; https://doi.org/10.3390/biom9080332
Received: 26 June 2019 / Revised: 30 July 2019 / Accepted: 30 July 2019 / Published: 1 August 2019
(This article belongs to the Section Biochemistry)
Endothelial cells form the inner lining of blood vessels, in a process known as angiogenesis. Excessive angiogenesis is a hallmark of several diseases, including cancer. The number of studies in endothelial cell metabolism has increased in recent years, and new metabolic targets for pharmacological treatment of pathological angiogenesis are being proposed. In this work, we wanted to address experimental evidence of substrate (namely glucose, glutamine and palmitate) dependence in immortalized dermal microvascular endothelial cells in comparison to primary endothelial cells. In addition, due to the lack of information about lactate metabolism in this specific type of endothelial cells, we also checked their capability of utilizing extracellular lactate. For fulfilling these aims, proliferation, migration, Seahorse, substrate uptake/utilization, and mRNA/protein expression experiments were performed. Our results show a high glycolytic capacity of immortalized dermal microvascular endothelial cells, but an early independence of glucose for cell growth, whereas a total dependence of glutamine to proliferate was found. Additionally, in contrast with reported data in other endothelial cell lines, these cells lack monocarboxylate transporter 1 for extracellular lactate incorporation. Therefore, our results point to the change of certain metabolic features depending on the endothelial cell line. View Full-Text
Keywords: endothelial cells; metabolism; glycolysis; lactate; MCT1 endothelial cells; metabolism; glycolysis; lactate; MCT1
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Ocaña, M.C.; Martínez-Poveda, B.; Quesada, A.R.; Medina, M.Á. Highly Glycolytic Immortalized Human Dermal Microvascular Endothelial Cells are Able to Grow in Glucose-Starved Conditions. Biomolecules 2019, 9, 332.

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