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Biomolecules 2019, 9(3), 110; https://doi.org/10.3390/biom9030110

Effect of Antifreeze Glycoproteins on Organoid Survival during and after Hypothermic Storage

1
AMOLF, Science Park 104, 1098 XG Amsterdam, The Netherlands
2
University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA
3
Max Planck Institute for Polymer Research, Ackermanweg 10, D-55128 Mainz, Germany
*
Author to whom correspondence should be addressed.
Received: 23 January 2019 / Revised: 8 March 2019 / Accepted: 15 March 2019 / Published: 19 March 2019
(This article belongs to the Special Issue Antifreeze Protein: New Insight from Different Approaches)
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Abstract

We study the effect of antifreeze glycoproteins (AFGPs) on the survival of organoids under hypothermic conditions. We find that the survival of organoids in cold conditions depends on their developmental stage. Mature organoids die within 24 h when being stored at 4 °C, while cystic organoids can survive up to 48 h. We find that in the presence of AFGPs, the organoid survival is prolonged up to 72 h, irrespective of their developmental stage. Fluorescence microscopy experiments reveal that the AFGPs predominately localize at the cell surface and cover the cell membranes. Our findings support a mechanism in which the positive effect of AFGPs on cell survival during hypothermic storage involves the direct interaction of AFGPs with the cell membrane. Our research highlights organoids as an attractive multicellular model system for studying the action of AFGPs that bridges the gap between single-cell and whole-organ studies. View Full-Text
Keywords: antifreeze glycoproteins; organoids; hypothermic storage; fluorescence microscopy antifreeze glycoproteins; organoids; hypothermic storage; fluorescence microscopy
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Huelsz-Prince, G.; DeVries, A.L.; Bakker, H.J.; van Zon, J.S.; Meister, K. Effect of Antifreeze Glycoproteins on Organoid Survival during and after Hypothermic Storage. Biomolecules 2019, 9, 110.

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