Genome-Wide Identification and Characterization of SQUAMOSA—Promoter-Binding Protein (SBP) Genes Involved in the Flowering Development of Citrus Clementina
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Materials
2.2. Identification and Molecular Cloning of CclSBPs
2.3. Phylogenetic Analysis of CclSBPs
2.4. Gene Structure, Conserved Domain and miR156 Target Site Analysis of CclSBPs
2.5. Identification of the Specific Repetitive Elements of CclSBPs
2.6. Co-Expression Analysis of CclSBPs During Floral Inductive Water Deficits
2.7. Real-Time Polymerase Chain Reaction (RT-PCR)
2.8. Arabidopsis Transformation
3. Results
3.1. Identification, Cloning and Sequence Feature Analysis of CclSBPs
3.2. Gene Structure, Conserved Domain and miR156 Target Site Analysis of CclSBPs
3.3. Comparative and Phylogenetic Analysis of the SBP-Box Genes in Various Plants
3.4. Specific Repetitive Elements in the CclSBPs
3.5. Analysis of the Expression Patterns of the CclSBPs Gene Family
3.6. Co-Expression Analysis of the CclSBPs Under Floral Inductive Water Deficits Conditions
3.7. Phenotypes of CclSBP6 Over-Expression in Arabidopsis
3.8. Alternative Splicing and Functional Analysis of CclSBP7 in Transgenic Arabidopsis
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Gene Name | Gene Locus | Genomic (bp) | CDs (bp) | No. of Transcript | EST Match | miR156 Target |
---|---|---|---|---|---|---|
CclSBP1 | Ciclev10009879m | 1247 | 393 | 1 | +a | 3′-UTR |
CclSBP2 | Ciclev10016841m | 2716 | 570 | 2 | +a | 3′-UTR |
CclSBP3 | Ciclev10017104m | 1265 | 432 | 1 | +b | 3′-UTR |
CclSBP4 | Ciclev10019546m | 3662 | 1674 | 2 | +a | exon |
CclSBP5 | Ciclev10018697m | 6422 | 2967 | 1 | +c | no |
CclSBP6 | Ciclev10020532m | 4055 | 1170 | 2 | +a | exon |
CclSBP7 | Ciclev10021106m | 2387 | 987 | 3 | +a | no |
CclSBP8 | Ciclev10031834m | 3660 | 1140 | 1 | +a | exon |
CclSBP9 | Ciclev10031270m | 5184 | 1536 | 2 | +a | exon |
CclSBP10 | Ciclev10032171m | 3272 | 939 | 1 | +b | exon |
CclSBP11 | Ciclev10031391m | 5362 | 1443 | 8 | +a | exon |
CclSBP12 | Ciclev10000100m | 5217 | 3309 | 1 | +a | no |
CclSBP13 | Ciclev10011938m | 2986 | 1167 | 2 | +a | exon |
CclSBP14 | Ciclev10004227m | 6489 | 3117 | 6 | +a | no |
CclSBP15 | Ciclev10004348m | 7069 | 2397 | 2 | +a | no |
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Zeng, R.-F.; Zhou, J.-J.; Liu, S.-R.; Gan, Z.-M.; Zhang, J.-Z.; Hu, C.-G. Genome-Wide Identification and Characterization of SQUAMOSA—Promoter-Binding Protein (SBP) Genes Involved in the Flowering Development of Citrus Clementina. Biomolecules 2019, 9, 66. https://doi.org/10.3390/biom9020066
Zeng R-F, Zhou J-J, Liu S-R, Gan Z-M, Zhang J-Z, Hu C-G. Genome-Wide Identification and Characterization of SQUAMOSA—Promoter-Binding Protein (SBP) Genes Involved in the Flowering Development of Citrus Clementina. Biomolecules. 2019; 9(2):66. https://doi.org/10.3390/biom9020066
Chicago/Turabian StyleZeng, Ren-Fang, Jing-Jing Zhou, Sheng-Rui Liu, Zhi-Meng Gan, Jin-Zhi Zhang, and Chun-Gen Hu. 2019. "Genome-Wide Identification and Characterization of SQUAMOSA—Promoter-Binding Protein (SBP) Genes Involved in the Flowering Development of Citrus Clementina" Biomolecules 9, no. 2: 66. https://doi.org/10.3390/biom9020066