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Open AccessArticle

Molecular Characterization of a Novel Family VIII Esterase with β-Lactamase Activity (PsEstA) from Paenibacillus sp.

by Sena Kwon 1,†, Wanki Yoo 1,2,†, Young-Ok Kim 3, Kyeong Kyu Kim 2 and T. Doohun Kim 1,*
1
Department of Chemistry, College of Natural Science, Sookmyung Women’s University, Seoul 04310, Korea
2
Department of Molecular Cell Biology, SungKyunKwan University School of Medicine, Suwon 2066, Korea
3
Biotechnology Research Division, National Fisheries Research and Development Institute, Gijang, Busan 46083, Korea
*
Author to whom correspondence should be addressed.
These two authors contributed equally to this study.
Biomolecules 2019, 9(12), 786; https://doi.org/10.3390/biom9120786
Received: 10 October 2019 / Revised: 19 November 2019 / Accepted: 24 November 2019 / Published: 26 November 2019
Molecular information about family VIII esterases, which have similarities with class C β-lactamases and penicillin-binding proteins, remains largely unknown. In this study, a novel family VIII esterase with β-lactamase activity (PsEstA) from Paenibacillus sp. was characterized using several biochemical and biophysical methods. PsEstA was effective on a broad range of substrates including tertiary butyl acetate, glyceryl tributyrate, glucose pentaacetate, olive oil, and p-nitrophenyl esters. Additionally, PsEstA hydrolyzed nitrocefin, cefotaxime, and 7-aminocephalosporanic acid. Interestingly, two forms of immobilized PsEstA (CLEAs-PsEstA and mCLEAs-PsEstA) showed high recycling property and enhanced stability, but hybrid nanoflowers (hNFs) of PsEstA require improvement. This study provides a molecular understanding of substrate specificities, catalytic regulation, and immobilization of PsEstA, which can be efficiently used in biotechnological applications. View Full-Text
Keywords: PsEstA; family VIII esterase; β-lactamase; antibiotics; immobilization PsEstA; family VIII esterase; β-lactamase; antibiotics; immobilization
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Kwon, S.; Yoo, W.; Kim, Y.-O.; Kim, K.K.; Kim, T.D. Molecular Characterization of a Novel Family VIII Esterase with β-Lactamase Activity (PsEstA) from Paenibacillus sp.. Biomolecules 2019, 9, 786.

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