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Open AccessArticle

Transcriptome Mechanism of Utilizing Corn Steep Liquor as the Sole Nitrogen Resource for Lipid and DHA Biosynthesis in Marine Oleaginous Protist Aurantiochytrium sp.

1
Institute of Biological Resources, Jiangxi Academy of Sciences, Nanchang 330096, China
2
Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, No.18, Chaowang Road, Hangzhou 310014, China
3
Department of Bioengineering, College of Chemical and Environmental Engineering, Shandong University of Science and Technology, Qingdao 266590, China
4
Marine Science and Engineering College, Qingdao Agricultural University, Qingdao 266109, China
*
Author to whom correspondence should be addressed.
Biomolecules 2019, 9(11), 695; https://doi.org/10.3390/biom9110695
Received: 26 September 2019 / Revised: 31 October 2019 / Accepted: 1 November 2019 / Published: 4 November 2019
In the current study, corn steep liquor (CSL) is evaluated as an ideal raw agro-material for efficient lipid and docosahexaenoic acid DHA production by Aurantiochytrium sp. Low CSL level in medium (nitrogen deficiency) stimulated the biosynthesis of lipids and DHA while inhibiting cellular growth. The transcriptomic profiles of the Aurantiochytrium sp. cells are analyzed and compared when cultured under high (H group), normal (N group), and low (L group) levels of CSL in the medium. The discriminated transcriptomic profiles from the three groups indicates that changes in CSL level in medium result in a global change in transcriptome of Aurantiochytrium sp. The overall de novo assembly of cDNA sequence data generated 61,163 unigenes, and 18,129 of them were annotated in at least one database. A total of 5105 differently expressed (DE) genes were found in the N group versus the H group, with 2218 downregulated and 2887 upregulated. A total of 3625 DE genes were found in the N group versus the L group, with 1904 downregulated and 1721 upregulated. The analysis and categorization of the DE genes indicates that the regulation mechanism of CSL involved in the perception and transduction of the limited nitrogen signal, the interactions between the transcription factors (TFs) and multiple downstream genes, and the variations in downstream genes and metabolites, in sequence, are illuminated for the first time in the current study.
Keywords: Aurantiochytrium sp.; nitrogen-deficiency; RNA-seq; corn steep liquor (CSL); signal transduction; DHA Aurantiochytrium sp.; nitrogen-deficiency; RNA-seq; corn steep liquor (CSL); signal transduction; DHA
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Wang, D.-S.; Yu, X.-J.; Zhu, X.-Y.; Wang, Z.; Li, H.-J.; Wang, Z.-P. Transcriptome Mechanism of Utilizing Corn Steep Liquor as the Sole Nitrogen Resource for Lipid and DHA Biosynthesis in Marine Oleaginous Protist Aurantiochytrium sp.. Biomolecules 2019, 9, 695.

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