Next Article in Journal
The Lysosomal Sequestration of Tyrosine Kinase Inhibitors and Drug Resistance
Previous Article in Journal
Illuminating Clues of Cancer Buried in Prostate MR Image: Deep Learning and Expert Approaches
Open AccessArticle

Biochemical Characterization of a Novel α/β-Hydrolase/FSH from the White Shrimp Litopenaeus vannamei

Laboratorio de Estructura Biomolecular. Centro de Investigacion en Alimentacion y Desarrollo, A.C. 83304 Hermosillo, Sonora, Mexico
Departamento de Investigación y Posgrado en Alimentos. Universidad de Sonora, 83000 Hermosillo, Sonora, Mexico
Departamento de Ciencias de la Salud, Universidad de Sonora, Cd. 85040 Obregon, Sonora, Mexico
Departamento de Ingeniería Celular & Biocatalisis, Instituto de Biotecnologia, Universidad Nacional Autonoma de Mexico, 62250 Cuernavaca, Morelos, Mexico
Authors to whom correspondence should be addressed.
Biomolecules 2019, 9(11), 674;
Received: 24 September 2019 / Revised: 28 October 2019 / Accepted: 29 October 2019 / Published: 31 October 2019
(This article belongs to the Section Enzymology)
(1) Background: Lipases and esterases are important enzymes that share the α/β hydrolase fold. The activity and cellular localization are important characteristics to understand the role of such enzymes in an organism. (2) Methods: Bioinformatic and biochemical tools were used to describe a new α/β hydrolase from a Litopenaeus vannamei transcriptome (LvFHS for Family Serine Hydrolase). (3) Results: The enzyme was obtained by heterologous overexpression in Escherichia coli and showed hydrolytic activity towards short-chain lipid substrates and high affinity to long-chain lipid substrates. Anti-LvFHS antibodies were produced in rabbit that immunodetected the LvFSH enzyme in several shrimp tissues. (4) Conclusions: The protein obtained and analyzed was an α/β hydrolase with esterase and lipase-type activity towards long-chain substrates up to 12 carbons; its immunodetection in shrimp tissues suggests that it has an intracellular localization, and predicted roles in energy mobilization and signal transduction. View Full-Text
Keywords: transcriptome; α/β hydrolases; esterase; lipase; immunoassays; shrimp transcriptome; α/β hydrolases; esterase; lipase; immunoassays; shrimp
Show Figures

Figure 1

MDPI and ACS Style

Garcia-Orozco, K.D.; Cinco-Moroyoqui, F.; Angulo-Sanchez, L.T.; Marquez-Rios, E.; Burgos-Hernandez, A.; Cardenas-Lopez, J.L.; Gomez-Aguilar, C.; Corona-Martinez, D.O.; Saab-Rincon, G.; Sotelo-Mundo, R.R. Biochemical Characterization of a Novel α/β-Hydrolase/FSH from the White Shrimp Litopenaeus vannamei. Biomolecules 2019, 9, 674.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

Back to TopTop