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Open AccessArticle

Biochemical Characterization of a Novel α/β-Hydrolase/FSH from the White Shrimp Litopenaeus vannamei

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Laboratorio de Estructura Biomolecular. Centro de Investigacion en Alimentacion y Desarrollo, A.C. 83304 Hermosillo, Sonora, Mexico
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Departamento de Investigación y Posgrado en Alimentos. Universidad de Sonora, 83000 Hermosillo, Sonora, Mexico
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Departamento de Ciencias de la Salud, Universidad de Sonora, Cd. 85040 Obregon, Sonora, Mexico
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Departamento de Ingeniería Celular & Biocatalisis, Instituto de Biotecnologia, Universidad Nacional Autonoma de Mexico, 62250 Cuernavaca, Morelos, Mexico
*
Authors to whom correspondence should be addressed.
Biomolecules 2019, 9(11), 674; https://doi.org/10.3390/biom9110674
Received: 24 September 2019 / Revised: 28 October 2019 / Accepted: 29 October 2019 / Published: 31 October 2019
(This article belongs to the Section Enzymology)
(1) Background: Lipases and esterases are important enzymes that share the α/β hydrolase fold. The activity and cellular localization are important characteristics to understand the role of such enzymes in an organism. (2) Methods: Bioinformatic and biochemical tools were used to describe a new α/β hydrolase from a Litopenaeus vannamei transcriptome (LvFHS for Family Serine Hydrolase). (3) Results: The enzyme was obtained by heterologous overexpression in Escherichia coli and showed hydrolytic activity towards short-chain lipid substrates and high affinity to long-chain lipid substrates. Anti-LvFHS antibodies were produced in rabbit that immunodetected the LvFSH enzyme in several shrimp tissues. (4) Conclusions: The protein obtained and analyzed was an α/β hydrolase with esterase and lipase-type activity towards long-chain substrates up to 12 carbons; its immunodetection in shrimp tissues suggests that it has an intracellular localization, and predicted roles in energy mobilization and signal transduction. View Full-Text
Keywords: transcriptome; α/β hydrolases; esterase; lipase; immunoassays; shrimp transcriptome; α/β hydrolases; esterase; lipase; immunoassays; shrimp
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Garcia-Orozco, K.D.; Cinco-Moroyoqui, F.; Angulo-Sanchez, L.T.; Marquez-Rios, E.; Burgos-Hernandez, A.; Cardenas-Lopez, J.L.; Gomez-Aguilar, C.; Corona-Martinez, D.O.; Saab-Rincon, G.; Sotelo-Mundo, R.R. Biochemical Characterization of a Novel α/β-Hydrolase/FSH from the White Shrimp Litopenaeus vannamei. Biomolecules 2019, 9, 674.

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