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Open AccessArticle

Immunotherapy of Hepatocellular Carcinoma with Magnetic PD-1 Peptide-Imprinted Polymer Nanocomposite and Natural Killer Cells

1
Department of Materials Science and Engineering, I-Shou University, Kaohsiung 84001, Taiwan
2
Department of Internal Medicine, Division of Cardiology, Zuoying Branch of Kaohsiung Armed Forces General Hospital, Kaohsiung 813, Taiwan
3
Department of Chemical and Materials Engineering, National University of Kaohsiung, Kaohsiung 81148, Taiwan
4
Department of Physics and Astronomy, University of New Mexico, Albuquerque, NM 87131, USA
*
Author to whom correspondence should be addressed.
These authors contributed equally to the work.
Biomolecules 2019, 9(11), 651; https://doi.org/10.3390/biom9110651
Received: 28 September 2019 / Revised: 23 October 2019 / Accepted: 23 October 2019 / Published: 25 October 2019
Programmed cell death protein 1 (PD-1) is a biomarker on the surface of cells with a role in promoting self-tolerance by suppressing the inflammatory activity of T cells. In this work, one peptide of PD-1 was used as the template for molecular imprinting to form magnetic peptide-imprinted poly(ethylene-co-vinyl alcohol) composite nanoparticles (MPIP NPs). The nanoparticles were characterized by dynamic light scattering (DLS), high-performance liquid chromatography (HPLC), Brunauer–Emmett–Teller (BET) analysis, and superconducting quantum interference device (SQUID) analysis. Natural killer 92 (NK-92) cells were added to these composite nanoparticles and then incubated with human hepatoma (HepG2) cells. The viability and the apoptosis pathway of HepG2 were then studied using cell counting kit-8 (CCK8) and quantitative real-time polymerase chain reaction (qRT-PCR), respectively. These nanoparticles were found to significantly enhance the activity of natural killer cells toward HepG2 cells by increasing the expression of nuclear factor kappa B (NF-κB), caspase 8, and especially caspase 3. View Full-Text
Keywords: immunotherapy; human hepatoma cells; programmed cell death protein 1 (PD-1); magnetic nanoparticles; peptide-imprinted polymer; natural killer cells immunotherapy; human hepatoma cells; programmed cell death protein 1 (PD-1); magnetic nanoparticles; peptide-imprinted polymer; natural killer cells
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Lee, M.-H.; Liu, K.-H.; Thomas, J.L.; Chen, J.-R.; Lin, H.-Y. Immunotherapy of Hepatocellular Carcinoma with Magnetic PD-1 Peptide-Imprinted Polymer Nanocomposite and Natural Killer Cells. Biomolecules 2019, 9, 651.

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