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Article

IGF2BP1 Significantly Enhances Translation Efficiency of Duck Hepatitis A Virus Type 1 without Affecting Viral Replication

1
College of Veterinary Medicine, Shandong Agricultural University, Taian 271000, Shandong, China
2
College of Public Health and Management, Weifang Medical University, Weifang 261042, Shandong, China
3
Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Taian 271000, Shandong, China
4
Molecular Virology Laboratory, VA-MD College of Veterinary Medicine and Maryland Pathogen Research Institute, University of Maryland, College Park, MD 20742, USA
5
Department of Basic Medical Sciences, Taishan Medical College, Taian 271000, Shandong, China
6
College of Animal Science and Technology, Shandong Agricultural University, Taian 271000, Shandong, China
*
Author to whom correspondence should be addressed.
Biomolecules 2019, 9(10), 594; https://doi.org/10.3390/biom9100594
Received: 29 July 2019 / Revised: 6 October 2019 / Accepted: 8 October 2019 / Published: 10 October 2019
As a disease characterized by severe liver necrosis and hemorrhage, duck viral hepatitis (DVH) is mainly caused by duck hepatitis A virus (DHAV). The positive-strand RNA genome of DHAV type 1 (DHAV-1) contains an internal ribosome entry site (IRES) element within the 5′ untranslated region (UTR), structured sequence elements within the 3′ UTR, and a poly(A) tail at the 3′ terminus. In this study, we first examined that insulin-like growth factor-2 mRNA-binding protein-1 (IGF2BP1) specifically interacted with the DHAV-1 3′ UTR by RNA pull-down assay. The interaction between IGF2BP1 and DHAV-1 3′ UTR strongly enhanced IRES-mediated translation efficiency but failed to regulate DHAV-1 replication in a duck embryo epithelial (DEE) cell line. The viral propagation of DHAV-1 strongly enhanced IGF2BP1 expression level, and viral protein accumulation was identified as the key point to this increment. Collectively, our data demonstrated the positive role of IGF2BP1 in DHAV-1 viral proteins translation and provided data support for the replication mechanism of DHAV-1. View Full-Text
Keywords: duck hepatitis A virus type 1; insulin-like growth factor-2 mRNA-binding protein-1; 3′ untranslated region; translation efficiency; viral propagation duck hepatitis A virus type 1; insulin-like growth factor-2 mRNA-binding protein-1; 3′ untranslated region; translation efficiency; viral propagation
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MDPI and ACS Style

Chen, J.; Zhang, R.; Lan, J.; Lin, S.; Li, P.; Gao, J.; Wang, Y.; Xie, Z.-J.; Li, F.-C.; Jiang, S.-J. IGF2BP1 Significantly Enhances Translation Efficiency of Duck Hepatitis A Virus Type 1 without Affecting Viral Replication. Biomolecules 2019, 9, 594. https://doi.org/10.3390/biom9100594

AMA Style

Chen J, Zhang R, Lan J, Lin S, Li P, Gao J, Wang Y, Xie Z-J, Li F-C, Jiang S-J. IGF2BP1 Significantly Enhances Translation Efficiency of Duck Hepatitis A Virus Type 1 without Affecting Viral Replication. Biomolecules. 2019; 9(10):594. https://doi.org/10.3390/biom9100594

Chicago/Turabian Style

Chen, Junhao, Ruihua Zhang, Jingjing Lan, Shaoli Lin, Pengfei Li, Jiming Gao, Yu Wang, Zhi-Jing Xie, Fu-Chang Li, and Shi-Jin Jiang. 2019. "IGF2BP1 Significantly Enhances Translation Efficiency of Duck Hepatitis A Virus Type 1 without Affecting Viral Replication" Biomolecules 9, no. 10: 594. https://doi.org/10.3390/biom9100594

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