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Variations of Alkaloid Accumulation and Gene Transcription in Nicotiana tabacum

College of Horticulture, Sichuan Agricultural University, Chengdu 611130, China
Zhengzhou Tobacco Research Institute, Zhengzhou 450001, China
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Biomolecules 2018, 8(4), 114;
Received: 3 August 2018 / Revised: 5 October 2018 / Accepted: 5 October 2018 / Published: 15 October 2018
PDF [1446 KB, uploaded 15 October 2018]


To increase the understanding of alkaloid biosynthesis in Nicotiana tabacum during whole plant growth periods, variations of the contents of alkaloids and the transcription of key biosynthetic genes in fresh leaves were investigated in three varieties at five developmental stages. Six alkaloids were analyzed by gas chromatograph–mass spectrometry (GC–MS) and the most abundant alkaloid was observed during the upper leaves maturing stage in the varieties, among which the alkaloid content of K326 was the highest. Considering the genetic effect, variance analysis indicated that the developmental stage played a predominant role in alkaloid accumulation. Moreover, the levels of biosynthetic gene transcripts in the leaves at the vigorous growing stage might contribute to the contents of alkaloids in the leaves during the maturing stages. To further illuminate the metabolism of alkaloid biosynthesis, a correlation among alkaloids was also documented. View Full-Text
Keywords: Nicotiana tabacum; alkaloids; gene transcription; developmental stages; varieties Nicotiana tabacum; alkaloids; gene transcription; developmental stages; varieties

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Sun, B.; Tian, Y.-X.; Zhang, F.; Chen, Q.; Zhang, Y.; Luo, Y.; Wang, X.-R.; Lin, F.-C.; Yang, J.; Tang, H.-R. Variations of Alkaloid Accumulation and Gene Transcription in Nicotiana tabacum. Biomolecules 2018, 8, 114.

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