In Vivo Acute on Chronic Ethanol Effects in Liver: A Mouse Model Exhibiting Exacerbated Injury, Altered Metabolic and Epigenetic Responses
Abstract
:1. Introduction
2. Results
2.1. Increased Hepatic Steatosis, Necrosis, Adenosine, CYP2E Levels and Decreased S-Adenosylmethionine by Binge Administration in Chronic Ethanol Treated Mice
2.2. Increased Phosphorylation of Histone H3 after Chronic Ethanol-Binge
2.3. Levels of Dimethylated H3 K4, Dimethylated H3 K9, and Trimethylated H3K9
2.4. Increased H3K9 Acetylation, H3K9 Acetylation/S10 Phosphorylation and H2AX Phosphorylation
2.5. Increased Nuclear Levels of GCN5, HDAC3 and Decreased Levels of Phospho-CREB in Chronic Ethanol, Binge and Chronic Ethanol-Binge Groups
3. Discussion
4. Experimental Section
4.1. Reagents
4.2. Experimental Design for Chronic Ethanol Feeding and Binge Administration
4.3. Biochemical and Histological Studies
4.4. Nuclear Isolation and Immunoblot Analysis
4.5. Data Analysis
5. Conclusions
Author Contributions
Conflicts of Interest
References
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Shukla, S.D.; Aroor, A.R.; Restrepo, R.; Kharbanda, K.K.; Ibdah, J.A. In Vivo Acute on Chronic Ethanol Effects in Liver: A Mouse Model Exhibiting Exacerbated Injury, Altered Metabolic and Epigenetic Responses. Biomolecules 2015, 5, 3280-3294. https://doi.org/10.3390/biom5043280
Shukla SD, Aroor AR, Restrepo R, Kharbanda KK, Ibdah JA. In Vivo Acute on Chronic Ethanol Effects in Liver: A Mouse Model Exhibiting Exacerbated Injury, Altered Metabolic and Epigenetic Responses. Biomolecules. 2015; 5(4):3280-3294. https://doi.org/10.3390/biom5043280
Chicago/Turabian StyleShukla, Shivendra D., Annayya R. Aroor, Ricardo Restrepo, Kusum K. Kharbanda, and Jamal A. Ibdah. 2015. "In Vivo Acute on Chronic Ethanol Effects in Liver: A Mouse Model Exhibiting Exacerbated Injury, Altered Metabolic and Epigenetic Responses" Biomolecules 5, no. 4: 3280-3294. https://doi.org/10.3390/biom5043280